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Biomolecules Test - 32

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Biomolecules Test - 32
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  • Question 1
    1 / -0
    Which of the following enzymes has/have heme as a prosthetic group?
    (i) Catalase                            
    (ii) Carboxypeptidase
    (iii) Succinic dehydrogenase 
    (iv) Peroxidase
    Solution
    • Co-enzymes tightly or covalently bonded to enzymes are called prosthetic groups, e.g., pyridoxal phosphate, heme, biotin. 
    • Heme is a prosthetic group containing ferrous ions. 
    • It is important for a number of biologically important hemoproteins such as myoglobin, cytochrome oxidase, catalase and peroxidase. 
    Thus, the correct answer is option E.
  • Question 2
    1 / -0
    Enzymes capable of changing their form are called as
    Solution
    Allosteric enzymes have an extra allosteric site in addition to the active site. Allosteric enzymes activity is modulated by allosteric modulators. The allosteric modulators bind to the allosteric site and bring about changes in the enzyme which modulates its confirmation. Thus, the correct answer is option D.

  • Question 3
    1 / -0
    Which enzyme shows greatest substrate specificity?
    Solution
    • Enzymes are highly specific catalysts and show high degree of substrate specificity. 
    • Some enzymes names are general and these enzymes act on a type of biomolecules. For example: nucleases mean enzymes acting on nucleic acids. Nuclease is a general class of enzymes. 
    • Similarly, pepsin and trypsin are protein metabolizing enzymes. These enzymes will act on any proteins and not on some specific protein. 
    • Sucrase is, however, a specific enzyme that will act on sucrose only.

    So, the correct answer 'Sucrase'
  • Question 4
    1 / -0
    The enzymes ribulose biphosphate carboxylase-oxygenase and phosphoenol pyruvate carboxylase are activated by
    Solution
    Both RUBISCO and PEP carboxylase are important enzymes of dark reaction of photosynthesis. RUBISCO catalyzes carboxylation in C3 plants. In the presence of magnesium activated RUBISCO, carbon dioxide combines with ribulose bis phosphate to form phosphoglyceric acid. In C4 plants, mesophyll cells fix carbon dioxide with the help of phospho enol pyruvate in the presence of magnesium activated phospho-enol pyruvate carboxylase.
  • Question 5
    1 / -0
    In which one of the following enzymes, is copper necessarily associated as an activator?
    Solution
    Some enzymes require metal ions for their activity. Tyrosinase is a copper containing enzyme that catalyzes the production of melanin and other pigments.
  • Question 6
    1 / -0
    Who proposed the principle of "Induced fit"?
    Solution
    • The induced-fit model is proposed by Daniel Koshland in 1958. It is the most accepted model for the enzyme-substrate complex. 
    • It describes the formation of the enzyme-substrate complex as a result of the interaction between the substrate and a flexible active site which means exposure of an enzyme to a substrate causes the active site of the enzyme to change shape in order to allow the enzyme and substrate to bind. 

    Hence option C is correct.
  • Question 7
    1 / -0
    Which one of the following belongs to transferase group?
    Solution
    Transaminase belongs to transferase group. Many amino acid biosynthetic pathways involve transamination reactions. The alpha-amino group from one amino acid is transferred to an alpha-keto acid to produce a new amino acid. This reaction is catalyzed by the enzyme called aminotransferases (formerly called transaminases). So, correct answer is B.
  • Question 8
    1 / -0
    Basically how many types of enzymes have been recognised by International Union of Biochemistry?
    Solution
    According to the modern classification system given by International Union of Biochemistry, enzymes have been classified into six classes as follows :
    Class First is Oxidoreductase.
    Class Second is Transferase.
    Class Third is Hydrolase.
    Class Fourth is Lyase.
    Class Fifth is Isomerase.
    Class Sixth is Ligase.
  • Question 9
    1 / -0
    In reference to enzymes, turnover number means
    Solution
    Enzymes are catalysts that speed up chemical reactions  without getting used up in the process. In reference to enzymes, turnover number means the maximum number of substrate molecule that is being converted  to product per second. It is represented by K$$_{cat}$$.
    Thus, the correct answer is 'The number of substrate molecules that a molecule of an enzyme converts into products per second.'
  • Question 10
    1 / -0
    Dry seeds can endure higher temperature than the germinating seeds because
    Solution
    Dry seeds in general are in a state of quiescence. The metabolic activity and consequently catalytic activity of enzymes is negligible. Hydration due to imbibition of water is the first stage of germination and initiates active metabolism in seed. Only after hydration, enzymes are fully active. Hence, hydration makes them sensitive to changes in temperature, pH, chemicals etc., like any other living entity.
    So, the correct answer is 'Hydration makes the enzymes more sensitive to temperature.'
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