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Biotechnology and its Applications Test - 27

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Biotechnology and its Applications Test - 27
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Weekly Quiz Competition
  • Question 1
    1 / -0
    Which ones are simple sequence repeats (SSRs)
    Solution
    Simple sequence repeats (SSRs), sometimes described as genetic 'stutters,' are DNA tracts in which a short base-pair motif is repeated several to many times in tandem (e.g. CAGCAGCAG). These sequences experience frequent mutations that alter the number of repeats similar to Multigene Family, a set of genes descended by duplication and variation from some ancestral gene.
    So, the correct answer is 'Multiple gene family.'
  • Question 2
    1 / -0
    Banting and Best successfully purified insulin from the pancreas of
    Solution
    In their experiment, Banting and Best removed the pancreas from a dog. Its level of blood glucose rose and it became weak as it developed diabetes. These scientists isolated the hormone insulin from the islets of Langerhans of the pancreas by various processes and purified it.
    Thus the correct answer is option B.
  • Question 3
    1 / -0
    Mexican dwarf varieties of wheat were developed by
    Solution
    C. Borlaug
    Solution : Mexican dwarf varieties were first produced by Norman Borlaug in Mexico to increase wheat yields for the country.
    So the correct answer is " Borlaug"
  • Question 4
    1 / -0
    In gene therapy, .......... can be treated by gene DNase.
    Solution
    • Cystic Fibrosis is a disease that affects the lungs. It is an autosomal recessive disease characterized by the excess secretion of thick and sticky mucus in the lungs.
    • A mutation in the cystic fibrosis transmembrane conductance regulator (CFTR) gene leads to cystic fibrosis. 
    • This disease can be treated by gene therapy using the enzyme DNase that is a DNA-breaking enzyme.
    • This enzyme breaks the DNA strands of the mucus generating cells thereby reducing muscus viscosity in the lungs of such patients.
    • Hence this process of gene therapy decreases the mucus generation in the lungs. 
      Thus the correct answer is option D.
  • Question 5
    1 / -0
    Human insulin is being commercially produced from a transgenic species of
    Solution
    Human insulin is produced from genetically engineered E.coli. By using genetic engineering or recombinant DNA technology, insulin-producing genes from human beings have been transferred into E.coli. bacteria, which produced insulin called 'humulin' for clinical use. This type of insulin was produced commercially by an American pharmaceutical firm Eli Lily on 5th July, 1983.

  • Question 6
    1 / -0
    After removal of signal peptide, the secretory protein involved in formation of insulin is known as
    Solution
    The insulin mRNA is translated as a single chain precursor called preproinsulin, and removal of its signal peptide during insertion into the endoplasmic reticulum generates proinsulin. Insulin and free C peptide are packaged in the Golgi into secretory granules which accumulate in the cytoplasm.
    So, the correct answer is 'Proinsulin.'
  • Question 7
    1 / -0
    PCR and RFLP are employed in ________.
    Solution
    B. Genetic engineering
    Solution : PCR and RFLP is used in detecting the genome of an unknown organism from a very small amount of DNA. PCR can amplify the DNA into a large amount and RFLP is used to analyze the DNA based on their restriction maps.
    So the correct answer is "Genetic engineering".
  • Question 8
    1 / -0
    What is indicated by $$'A'$$ in given figure?

    Solution
    The A in the figure indicates a disulfide bond/bridge formed between two proteins/polypeptides/peptides which have been synthesized using rDNA technology.  A disulfide bond is formed between the 2 cysteine (amino acid) residues present on the same or different polypeptide chains. It is very common in proteins and helps to hold the oligomers of a multimeric protein together. It can be seen in insulin, antibody structure, etc. 
    So, the correct answer is option C. 

  • Question 9
    1 / -0
    Number of rice varieties developed by ancient Indians was
    Solution
    Answer is option A i.e. "1,00,000"
    India was home to more than 1,00,000 rice varieties, which displayed diversity in taste, nutrition, pest-resistance and adaptability to a range of conditions.
  • Question 10
    1 / -0
    Expand ELISA.
    Solution
    ELISA is also known as enzyme linked immunosorbent assay. It is used to detect the presence of antigen and antibody in a given sample.
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