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Photosynthesis in Higher Plants Test - 79

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Photosynthesis in Higher Plants Test - 79
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  • Question 1
    1 / -0
    Photosynthesis may be studied in a number of ways. For this protocol, a dye-reduction technique is used. This dye-reduction investigation tests the hypothesis that light and chloroplasts are required for the light reactions to occur. A compound, DPIP (2,6-dichlorophenol-indophenol), will be substituted so that when a reduction reaction has occurred, the DPIP changes from blue to colorless.
    In this experiment, chloroplasts are extracted from spinach leaves and incubated with DPIP in the presence of light. As the DPIP is reduced and becomes colorless, the resultant increase in light transmittance (at 605 nm, the A$$_{max}$$ of blue DPIP) is measured over a period of time using a spectrophotometer.
    Which molecule does DPIP replace in the light reactions of photosynthesis?
    Solution
    NADP$$^+$$ accepts protons from photolysis of water and is reduced to NADPH$$_2$$, which is transferred or used to reduce CO$$_2$$ during the light independent reaction.
    Ferredoxin is an electron acceptor
    ATP is energy storing compound
    Chlorophyll harvests light
    So, the correct answer is NADP$$^+$$

  • Question 2
    1 / -0

    By which of the following processes ATP is produced?

    I.Photosynthesis
    II.Aerobic respiration
    III. Anaerobic respiration
    Solution
    Photosynthesis is the fixation of atmospheric carbon dioxide into organic compounds; use of water as the electron donor by photoautotrophs during photophosphorylation results in the production of oxygen. Light reaction of photosynthesis includes trapping of Sunlight by chlorophyll a and other accessory pigments and production of ATP and NADPH. Aerobic respiration is the breakdown of sugar/other food substances in the presence of oxygen to released energy. It includes glycolysis, Krebs cycle and electron transport chain. Glycolysis and Kreb cycle produce reducing compounds NADH, FADH$$_2$$ which are then oxidized in the presence of oxygen by the process of chemiosmosis to produce a large amount of ATPs. Anaerobic respiration/Fermentation is incomplete oxidation of food substances into alcohol and lactic acid with small amount of energy being released. This makes option E correct answer. 
  • Question 3
    1 / -0
    Read the passage and answer the following question.
    An experiment is set up with a culture of algae undergoing normal photosynthesis. A pulse of radioactive carbon, in the form of $$^{14}$$CO$$_2$$, is inserted into the culture at time zero. At five second intervals, some of the cells are removed by dropping them into boiling water to halt metabolic processes. 
    The first organic material that would be identified as being radioactive during this process is
    Solution

    Photosynthetic algae carry out fixation of carbon dioxide into glucose wherein the first step includes carboxylation of RuBP into unstable 6C compounds which are immediately broken down into 2 molecules of 3C compounds called as phosphoglyceraldehyde (PGA). The follow-up reactions of Calvin cycle leads to the formation of one glucose molecule by three successive cycles. After five seconds, the radioactive carbon will be present in PGA only as the formation of glucose takes 3 successive Calvin cycles. Acetyl CoA is produced during cellular respiration of glucose, photosynthetic carbon is not incorporated into ATP and ADP. So, the correct answer is B.  

  • Question 4
    1 / -0
    The curve showing the amount of light absorbed at each wavelength is called 
    Solution
    • There are photosynthetic pigments in the chloroplast of the plant cells. These pigments absorb the light of a certain wavelength and utilize this light for performing photosynthesis. 
    • The spectrum of the absorption can be recorded by studying the reactions and the pigments involved. 
    • The curve representing the light absorbed at each wavelength by the pigment is called the absorption spectrum.
    • The curve showing rate of photosynthesis at different wavelengths of light is called the action spectrum.
    • Quantum yield in photosynthesis is the moles of CO$$_2$$ fixed per mole of quanta (photons) absorbed.
    • The number of photons or quanta required by a plant or leaf to release one molecule of oxygen during photosynthesis is called quantum requirement.
    • So, the correct answer is option B.

  • Question 5
    1 / -0
    Chlorophyll molecules are essential for photosynthesis, and allow plants, fungi, and some bacteria to capture free energy from light and convert it into chemical energy.
    An absorption spectrum illustrates the amount of light energy absorbed across a range of wavelengths , and the graph below represents the absorption spectra of chlorophyll a and chlorophyll b pigment molecules in a plant cell. The relative absorption rates of chlorophyll a and chlorophyll b indicate that

  • Question 6
    1 / -0
    Choose the correct statement that explains the role of CAM (Crassulacean Acid Metabolism) in plants?
    Solution
    CAM or Crassulacean Acid Metabolism is a photosynthetic process found in plants living in arid habitats. The carbon fixation pathway in CAM photosynthetic process is an evolved one adapted to meet the arid conditions wherein the stomata in the leaves remain closed during the day so as to reduce evapotranspiration, but are open at night to collect carbon dioxide.
    So, the correct answer is option C.
  • Question 7
    1 / -0
    Chemiosmotic theory of ATP synthesis in mitochondria is based on 
    Solution
    Peter Mitchell proposed that an electrochemical concentration gradient of protons across a membrane could be utilized to make ATP. He linked this process to osmosis, the diffusion of water across a membrane, this is the reason it is called chemiosmosis. More specifically, it relates to the generation of ATP by the movement of hydrogen ions across a membrane during cellular respiration. Hydrogen ions (protons) will diffuse from an area of high proton concentration to an area of lower proton concentration.
    So the correct option is '$$H^+$$ gradient'.
  • Question 8
    1 / -0
    Coupling factor- F occurs over
    Solution
    ATPase complex (also known as Coupling factor):
    The ATPase enzyme consists of two parts:
    a) F$$_0$$: It is embedded in the membrane and forms a transmembrane channel in thylakoid that carries out facilitated diffusion of protons across the membrane.
    b) F$$_1$$: It is protruding on the outer surface of the thylakoid membrane on the side that faces the stroma.
    Thylakoid membrane itself is not permeable to protons. The protons, therefore, flow towards the outside of thylakoid membrane through ATPase complex.
    Hence, the correct answer is 'Thylakoids'.
  • Question 9
    1 / -0
    The spaces inside the folds of ER membrane are known as
    Solution
    Thylakoids are membrane bound compartments inside chloroplast and cyanobacteria. 
    cisternae is a flattened membrane bound structure in  the endoplasmic reticulum and Golgi apparatus.
    Mesosomes are the invaginated structures formed by the localised infolding of the plasma membrane. 
    Periplasmic space is a concentrated gel like matrix  in the space between  the inner cytoplasmic membrane and  the bacterial outer membrane
    So the answer is cisternae option B
  • Question 10
    1 / -0
    How many are autotrophs : Lactobacillus, Nostoc, Chara, Nitrosomonas, Streptomyces, Saccharomyces, Trypanosoma, Porphyra, Wolffia?
    Solution
    Autotrophs are the organism that can manufacture their own food using materials from inorganic sources. These are also known as the producers and they are divided into two categories; the photoautotrophs and chemoautotrophs. Few examples are plants, green algae that includes the cyanobacteria and all the archaebacteria.
    From the given options the organisms that are not autotrophic are Nitrosomonas, Trypanosoma, Porphyra, Streptomuces and Saccharomyces.
    So, the correct option is 'Five'
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