Self Studies
Self Studies
Self Studies
Self Studies
Selfstudy
Selfstudy

Biotechnology Principles and Processes Test - 13

Result Self Studies

Biotechnology Principles and Processes Test - 13
  • Score

    -

    out of -
  • Rank

    -

    out of -
TIME Taken - -
Self Studies

SHARING IS CARING

If our Website helped you a little, then kindly spread our voice using Social Networks. Spread our word to your readers, friends, teachers, students & all those close ones who deserve to know what you know now.

Self Studies Self Studies
Weekly Quiz Competition
  • Question 1
    1 / -0
    A gene whose expression helps to identify transformed cell is known as
    Solution
    In recombinant DNA technology, selectable markers are the specific genes that are used to identify the transformants from the non-transformants after the process of recombination. These genes are used to detect whether the incorporation of a nucleic acid sequence has been successful into an organism's DNA. These marker genes are resistant to various antibiotics and are used in cloning vectors. Thus the correct answer is option B.
  • Question 2
    1 / -0
    In vitro clonal propagation in plants is characterized by:
    Solution
    • Molecular techniques are at present powerful and valuable tools for assessing the genetic stability among in vitro micropropagated plants. 
    • Random amplified polymorphic DNA (RAPD) is a polymerase chain reaction (PCR) based technique widely used for the determination of the genetic fidelity of in vitro regenerated plantlets. 
    • RAPD does not require prior sequence information, use a small amount of DNA, and are easy to use, thus considered to be suitable techniques for assessment of genetic fidelity of in vitro regenerated plantlets. 
    • So, the correct answer is option A.
  • Question 3
    1 / -0
    The taq polymerase enzyme is obtained from
    Solution
    Taq polymerase is a thermostable DNA polymerase named after the thermophilic bacterium Thermus aquaticus from which it was originally isolated by Thomas D. Brock in 1965.
  • Question 4
    1 / -0
    Which of the following restrictions enzymes produces blunt ends?
    Solution
    Restriction enzymes are the special enzymes which can recognise sites in the DNA sequences and make a cut at these sites. Restriction enzymes cut the DNA in middle leaving blunt ends on the DNA or staggered cuts are made that leave sticky ends with 5’or 3’ overhangs. These sticky overhangs can reanneal spontaneously by complementary base pairing. The restriction site of Eco RV is GAT_ATC and it produces blunt ends. The adaptor molecules are used to create sticky ends at the fragments which are cleaved with the help of this enzyme. 
    Thus, the correct answer is option D.
  • Question 5
    1 / -0
    Stirred-tank bioreactors have been designed for
    Solution

    Correct Answer: A
    Explanation:

    • Stirred tank bioreactors enable for the testing of tiny amounts of sample and the usage of a temperature and pH control system.
    • During the procedure, these bioreactors guarantee that oxygen is available.
    • To enable mixing of the reaction components, stirred-tank bioreactors are generally cylindrical or have a curved base. 
    • The stirrer ensures that the bioreactor is evenly mixed and that oxygen is available throughout. 
    • Alternatively, the reactor can be bubbled with air.

  • Question 6
    1 / -0
    An analysis of chromosomal DNA using the southern hybrization technique does not use
    Solution
    The steps of southern hybridization are as follows:
    • DNA or genomic source is digested with a restriction enzyme and separated by electrophoresis. The DNA is denatured into single strands by incubation with NaOH.
    • The DNA is transferred to a membrane which is a sheet of special blotting paper. The DNA fragments retain the same pattern of the separation as on the gel (blotting).
    • The blot is incubated with many copies of a probe which is single-stranded DNA. This probe will form base pairs with its complementary DNA sequence and bind to form a ds DNA molecule. the probe is radioactive.
    • The location of the probe is revealed when it is exposed to X-ray film (autoradiography).
    It does not involve PCR (polymerase chain reaction).

    So, the correct answer is 'PCR'.
  • Question 7
    1 / -0
    DNA fragments are
    Solution
    Answer: (C) Negatively charged.
    Explanation:

    • DNA is made up of deoxyribose sugar, phosphate group, and nitrogenous bases.
    • Phosphorus groups are negatively charged which imparts a negative charge to the DNA.
    • The charge of DNA and DNA fragments is the same. 

  • Question 8
    1 / -0
    The cutting of DNA at specific locations became possible with the discovery of
    Solution
    Genetic engineering is possible because of special enzymes that cut DNA. These enzymes are called restriction enzymes or restriction endonucleases. Restriction enzymes are proteins produced by bacteria to prevent or restrict invasion by foreign DNA. They act as DNA scissors, cutting the foreign DNA into pieces so that it cannot function. These enzymes are routinely used for DNA modification in laboratories and are a vital tool in molecular cloning
    So, the correct answer is 'Restriction enzymes'
  • Question 9
    1 / -0
    Which of the following is not a component of downstream processing?
    Solution
    Downstream processing is the recovery of biological products which includes the stages - extraction, separation, purification and preservation of the biotechnological products. The product is formulated with suitable preservatives. Therefore, the correct answer is option B.
  • Question 10
    1 / -0
    The process of separation and purification of expressed protein before marketing is called as ?
    Solution
    (A) Correct answer (C)
    (B) Explanation of correct answer:

    Downstreaming process helps in the recovery and purification of biosynthetic product. It is mainly used during large scale production of metabolites. The steps included in down streaming process are as follows:
    • Removal of insoluble substances
    • Isolation of product
    • Purification of product
    • Product polishing
    • So, the correct answer is 'Downstream processing'
Self Studies
User
Question Analysis

  • Correct -

  • Wrong -

  • Skipped -

My Perfomance
  • Score

    -

    out of -
  • Rank

    -

    out of -
Re-Attempt Weekly Quiz Competition
Self Studies Get latest Exam Updates
& Study Material Alerts!
No, Thanks
Self Studies
Click on Allow to receive notifications
Allow Notification
Self Studies
Self Studies Self Studies
To enable notifications follow this 2 steps:
  • First Click on Secure Icon Self Studies
  • Second click on the toggle icon
Allow Notification
Get latest Exam Updates & FREE Study Material Alerts!
Self Studies ×
Open Now