Biotechnology Principles and Processes Test

Result

Biotechnology Principles and Processes Test - 21

Score
-
out of -
Rank
-
out of -

TIME Taken - -

SHARING IS CARING

If our Website helped you a little, then kindly spread our voice using Social Networks. Spread our word to your readers, friends, teachers, students & all those close ones who deserve to know what you know now.

Weekly Quiz Competition

Question 1
1 / -0

Polyethylene glycol method is used for :

A
Energy production from sewage

B
Gene transfer without a vector

C
Biodiesel production

D
Seedless fruit production

Solution

Polyethylene glycol method is used for gene transfer without a vector. Introduction of DNA into plant cell without the involvement of a biological agent and leading to stable transformation is known as direct gene transfer. There are various methods for direct gene transfer one of which is chemical method. Certain chemicals e.g. PEG polyethylene Glycol, polyvinyl alcohol and calcium phosphate enhance the uptake of DNA by plant protoplast. PEG and calcium phosphate are thought to precipitate the DNA onto the outer surface of plasmalemma and the precipitate is taken up by the endocytosis.
So, the correct option is option B.
Question 2
1 / -0

Assertion: Recognition site should be preferably single and responsive to commonly used restriction enzyme.

Reason: In pBR 322 alien DNA is ligated generally in the area of Bam-HI site of tetracycline resistance gene.

A
If both the assertion and the reason are true and the reason is a correct explanation of the assertion

B
If both the assertion and reason are true but the reason is not a correct explanation of the assertion

C
If the assertion is true but the reason is false

D
If both the assertion and reason are false

E
If the assertion is false but reason is true
Question 3
1 / -0

Which of the following enzymes cut the DNA molecule at specific nucleotide sequence?

A
Restriction endonuclease

B
DNA ligase

C
RNA polymerase

D
Exonuclease

Solution

Restriction endonucleases recognise specific sequences in the DNA and cut the DNA into fragments. The specific sites are 4-6 bp long and are called as restriction sites. Each restriction endonuclease has a specific restriction site. Ligase enzyme joins two large molecules by forming a new chemical bond, e.g., DNA ligase joins two DNA fragments together via phosphodiester bond between two nucleotides. Exonucleases enzymes remove nucleotides one at a time from the end of a polynucleotide chain. They shorten the DNA by one nucleotide at a time, they do not digest it into fragments. RNA polymerase is a DNA-dependent RNA polymerase that copies the template DNA strand into primary transcript RNA through transcription. It extends the primer into RNA transcript, does not cut the DNA.
So, the correct answer is option A.
Question 4
1 / -0

Given below is a sample of a portion of DNA strand giving the base sequence on the opposite strands. What is so special shown in it?

5'-------GAATTC------3'
3'-------CTTAAG------5'

A
Palindromic sequence of base pairs

B
Replication completed

C
Deletion mutation

D
Start codon at the 5' end

Solution

If a nucleic acid sequence (DNA or RNA) is read same from 5' to 3' on one strand and 5' to 3' on its complementary strand, the sequence is called as palindromic sequence. In the question, the base sequence is same whether read from 5' to 3' upper strand (5' GAATTC 3') or 5' to 3' (5' GAATTC 3') on the lower strand. Correct answer is A.
Question 5
1 / -0

A genetically engineered bacteria used for clearing oil spills is

A
Escherischia coli

B
Bacillus subtilis

C
Agrobacterium tumifaciens

D
Pseudomonas putida

Solution

Pseudomonas putida is a Gram-negative, rod shaped, aerobic, chemoheterotrophic bacterium. Being chemoheterotrophic, they use organic compounds as source of their energy. This bacterium can break down any of aliphatic and aromatic hydrocarbons. Most of the organic toxins are aliphatic and aromatic hydrocarbons. Genetically engineered marine-dweller Pseudomonas putida use hydrocarbons as fuel and break down the ring structures of the hydrocarbons in oil spill in presence of enzymes and seawater oxygen and emit carbon dioxide.
so, the correct answer is 'Pseudomonas putida'
Question 6
1 / -0

Plant tissues can be separated into single cells by using

A
Cellulase and pectinase

B
Cellulase and auxins

C
Cellulase and peg

D
Auxins and cytokinins

Solution

Plant cells are surrounded with a cellulosic cell wall. Between adjacent plant cells is present middle lamella. The middle lamella is made up of calcium and pectin. Hence, plant cells of a tissue are separated from each other with the help of cellulase and pectinase enzymes. These enzymes are commercially used in clearing of fruit juices to dissolve plant fibres.
Question 7
1 / -0

In genetic engineering, the antibiotics are used ___________.

A
As selectable markers

B
To select healthy vectors

C
As sequence from where replication starts

D
To keep the cultures free of infection

E
Both A and D

Solution

In genetic engineering the selectable markers help us to select the cells that have lost the antibiotic genes associated with a specific antibiotic resistance after inserting the insert DNA among the group of wild type cells.
So the correct answer is "As selectable markers".
Question 8
1 / -0

Restriction endonuclease

A
synthesizes DNA

B
Cuts the DNA molecule randomly

C
Cuts the DNA molecule at specific sites

D
Restricts the synthesis of DNA inside the nucleus

Solution

A restriction enzyme is a protein that recognises a specific, short nucleotide sequence and cuts the DNA only at that specific site, which is known as restriction site or target sequence. A bacterium is immune to its own restriction enzymes, even if it has the target sequences ordinarily targeted by them. This is because the bacterial restriction sites are highly methylated, making them unrecognizable to the restriction enzyme. When a restriction enzyme cleaves a restriction site, the reaction creates highly reactive "sticky ends" on the broken DNA. This is useful to the biotechnologist .By cutting open vector DNA with the same restriction enzymes used to cleave the target DNA, complementary "sticky ends" are created. The fragment is "glued in" with DNA ligase, which creates the phosphodiester bonds necessary to complete the sugar phosphate backbone of the new recombinant DNA.
So, the correct option is option C.
Question 9
1 / -0

Taq polymerase which is used for amplification of DNA is related with

A
Hybridoma technique

B
PCR technique

C
Gene cloning

D
rDNA technology

Solution

Fusion of an antibody-producing B cell with a myeloma cell (with the ability to grow in tissue culture and lack antibody chain synthesis) produce hybrid cell lines that have the capacity to produce monoclonal antibodies and to grow in tissue culture indefinitely, thereby providing a constant supply of specific monoclonal antibodies.
Gene cloning refers to the production of multiple copies of a piece of DNA after its insertion into a vector. The vector is taken up by a cell and cloning occurs as the vector replicates.
r-DNA technology is the introduction of foreign genetic material into the genome of an organism that was not present before using restriction enzymes, DNA ligase and vectors.
PCR - technique amplify the gene of interest in three steps namely denaturation of target DNA (thermal cycle to separate the DNA strands), annealing of primers to the ssDNA and polymerisation (extension of primer into complete DNA strand complementary to the template strand). Taq polymerase is DNA polymerase from a hot-springs bacterium, Thermus aquaticus, that can withstand the denaturing temperatures. Therefore, omits the need of adding DNA polymerase, after each cycle of replication, to the reaction mixture and allow the cycling to be continued without interruption in PCR machines.
So, the correct answer is option B.
Question 10
1 / -0

Which scientist obtained interferon through recombinant DNA technology?

A
Kohler and Milstein

B
Charles Weismann

C
Nathans and Smith

D
Maurice Wilkins

Solution

Interferons are the glycoproteins that are secreted by a host cell to serve as signalling molecules. They block the multiplication of viruses in the cells by inducing the immune response of the neighbouring cell. Engineered interferons are used in medicines to treat cancer and other diseases. Charles Weissmann clone the human interferon $$\alpha$$ genes and produce interferon in bacteria.

User

Question Analysis

Correct -
Wrong -
Skipped -

My Perfomance

Score
-
out of -
Rank
-
out of -

Re-Attempt Weekly Quiz Competition

Biotechnology Principles and Processes Test - 21

Result

Biotechnology Principles and Processes Test - 21

Score

-

Rank

-

SHARING IS CARING

Question 1

Energy production from sewage

Gene transfer without a vector

Biodiesel production

Seedless fruit production

Solution

Question 2

If both the assertion and the reason are true and the reason is a correct explanation of the assertion

If both the assertion and reason are true but the reason is not a correct explanation of the assertion

If the assertion is true but the reason is false

If both the assertion and reason are false

If the assertion is false but reason is true

Question 3

Restriction endonuclease

DNA ligase

RNA polymerase

Exonuclease

Solution

Question 4

Palindromic sequence of base pairs

Replication completed

Deletion mutation

Start codon at the 5' end

Solution

Question 5

Escherischia coli

Bacillus subtilis

Agrobacterium tumifaciens

Pseudomonas putida

Solution

Question 6

Cellulase and pectinase

Cellulase and auxins

Cellulase and peg

Auxins and cytokinins

Solution

Question 7

As selectable markers

To select healthy vectors

As sequence from where replication starts

To keep the cultures free of infection

Both A and D

Solution

Question 8

synthesizes DNA

Cuts the DNA molecule randomly

Cuts the DNA molecule at specific sites

Restricts the synthesis of DNA inside the nucleus

Solution

Question 9

Hybridoma technique

PCR technique

Gene cloning

rDNA technology

Solution

Question 10

Kohler and Milstein

Charles Weismann

Nathans and Smith

Maurice Wilkins

Solution

User

Question Analysis

My Perfomance

Score

-

Rank

-

Results Announcement on: coming Monday

Stay Tuned: We’ll update you on your result via email or SMS.