Biotechnology Principles and Processes Test

Result

Biotechnology Principles and Processes Test - 24

Score
-
out of -
Rank
-
out of -

TIME Taken - -

SHARING IS CARING

If our Website helped you a little, then kindly spread our voice using Social Networks. Spread our word to your readers, friends, teachers, students & all those close ones who deserve to know what you know now.

Weekly Quiz Competition

Question 1
1 / -0

Polyethylene glycol method is used for

A
Seedless fruit production

B
Energy production from sewage

C
Gene transfer without a vector

D
Biodiesel production

Solution

Polyethylene glycol (PEG) is a polyether compound. The compound has varied applications. Its biological uses include being used as a precipitant for plasmid DNA isolation and protein crystallisation. PEG is also used as a fusing agent in the transformation experiments. The gene or the desired fragment of DNA can be transferred into the host cell without the use of vector. PEG with calcium ions allows the membrane to become charged and allow the entry of the gene.
Thus, the correct answer is option C.
Question 2
1 / -0

"Upstream process" in preparation of alcohol in biotechnology includes

A
All processes before fermentation

B
All processes during fermentation

C
All processes after fermentation

D
Flavouring and ripening of alcohol

Solution

"Upstream process" in preparation of alcohol in biotechnology includes all process before fermentation.
Therapeutic cell manufacturing processes can be separated into upstream processes and downstream processes. The upstream process is defined as the entire process from early cell isolation and cultivation, to cell banking and culture expansion of the cells until final harvest (termination of the culture and collection of the live cell batch). The upstream part of a bioprocess refers to the first step in which microbes/cells are grown, e.g., bacterial or mammalian cell lines, in bioreactors. Upstream processing involves all the steps related with inoculum development, media development, improvement of inoculum by genetic engineering process, optimization of growth kinetics so that product development can improve tremendously. Fermentation has two parts: upstream and downstream.
Thus, the correct answer is option A.
Question 3
1 / -0

Which one of the following acids was produced by fermentation process?

A
Citric acid

B
Lactic acid

C
Acetic acid

D
All of the above

Solution

Organic acids are among the most versatile ingredients in food and beverage industries. Citric acid, acetic acid, lactic acid, tartaric acid, malic acid, gluconic acid, propionic acid and fumaric acid are some of the organic acids used widely in various industries. They are obtained as the end-products or sometimes as the intermediate components of a particular biochemical cycle. Generally, organic acids are produced commercially either by chemical synthesis or fermentation. However, fermentation processes are the most commonly used method.
Thus, the correct answer is option D.
Question 4
1 / -0

What are important products of yeast fermentation?

A
Ethanol and acetic acid

B
Butanol and glycerol

C
Phenethyl alcohol, amyl alcohol and caproic acid

D
All of the above
Solution
- Fermentation is a metabolic process that converts sugar to acids, gases or alcohol anaerobically. It occurs in yeast and bacteria, but also in oxygen-starved muscle cells, as in the case of lactic acid fermentation.
- By fermentation, the yeast species Saccharomyces cerevisiae converts carbohydrates to carbon dioxide, acetic acid and alcohols like ethanol, butanol, phenethyl and amyl alcohol.
Yeast produces carporic acid by the fermentation of corn.

Thus, the correct answer is option D.
Question 5
1 / -0

Stirred-tank bioreactors have been designed for?

A
Availability of Oxygen throughout the process

B
Addition of preservatives to the product

C
Purification of the product

D
Ensuring anaerobic conditions in the culture vessel

Solution

Correct option: A
Explanation:

Stirred tank bioreactor is a reactor that consists of the propeller, the mixer and the reactor. In this system, impeller or mixer is used to mix the required atmospheric oxygen into the aqueous phase and maximizing the interfacial area between the gaseous and aqueous phases for large production of biomass.
Question 6
1 / -0

The figure below shows three steps (A, B, C) of polymerase chain reaction (PCR). Select the option giving correct identification together with what it represents?

A
A- Denaturation at a temperature of about 98$$^o$$C separating the two DNA strands.

B
A- Denaturation at a temperature of about 50$$^o$$C.

C
C- Extension in the presence of heat stable DNA polymerase.

D
B- Annealing with two sets of primers.

Solution

PCR or polymerase chain reaction is the technique employed for the amplification of the gene of interest. The image shows 3 steps of the PCR cycle, namely denaturation, annealing, and extension. Part A represents the step of denaturation, where the dsDNA is divided into two ssDNA under high temperature. For denaturation to occur, an approximate temperature of 90-92$$^o$$C is required.
Whereas, part B represents the attachment of short oligonucleotide sequences called primer by a process called annealing and part C represents the extension of the DNA segment by the addition of nucleotides by the action of the thermostable polymerase called Taq polymerase. Thus, the correct answer is option A.
Question 7
1 / -0

Stirred tank bioreactors have been designed for

A
Purification of the product

B
Ensuring anaerobic conditions in the culture vessel

C
Availability of oxygen throughout the process

D
Addition of preservatives to the product

Solution

Stirred tank bioreactors allow taking small volumes of the sample for testing and use of control system that regulates the temperature and pH. These bioreactors ensure oxygen availability during the process. The correct answer is C.
Question 8
1 / -0

Recombination of genes occurs at

A
Prophase of mitosis

B
Prophase I of meiosis

C
Metaphase II of meiosis

D
Prophase II of meiosis

Solution

Recombination occurs when two molecules of DNA exchange pieces of their genetic material with each other, resulting in the production of a new combination of alleles. Genetic recombination occurs in the pachytene stage of prophase I of meiosis during a process called as synapsis.
Therefore, the correct answer is option B.
Question 9
1 / -0

At which of the following stage after fermentation, the desired product is screened and purified?

A
Up stream processing

B
Screening

C
Down stream processing

D
Batch-culture

Solution

Downstream Processing is the recovery and purification of biochemical products with proper treatment. It is a series of events which includes cell separation, filtration, product recovery, extraction of product and purification and then treatment of product by chemical, physical and biological means. Thus, the correct answer is option C.
Question 10
1 / -0

Utilization of living organisms, systems and processes for producing materials useful to human society is known as

A
Applied biology

B
Technology

C
Biotechnology

D
Biomedical engineering

Solution

Biotechnology is the use of living systems and organisms to develop or make products, or "any technological application that uses biological systems, living organisms or derivatives thereof, to make or modify products or processes for specific use" (UN Convention on Biological Diversity, Art. 2). New tools and products developed by biotechnologists are useful in research, agriculture, industry and the clinic. Thus, the correct answer is option C.

User

Question Analysis

Correct -
Wrong -
Skipped -

My Perfomance

Score
-
out of -
Rank
-
out of -

Re-Attempt Weekly Quiz Competition

Biotechnology Principles and Processes Test - 24

Result

Biotechnology Principles and Processes Test - 24

Score

-

Rank

-

SHARING IS CARING

Question 1

Seedless fruit production

Energy production from sewage

Gene transfer without a vector

Biodiesel production

Solution

Question 2

All processes before fermentation

All processes during fermentation

All processes after fermentation

Flavouring and ripening of alcohol

Solution

Question 3

Citric acid

Lactic acid

Acetic acid

All of the above

Solution

Question 4

Ethanol and acetic acid

Butanol and glycerol

Phenethyl alcohol, amyl alcohol and caproic acid

All of the above

Solution

Question 5

Availability of Oxygen throughout the process

Addition of preservatives to the product

Purification of the product

Ensuring anaerobic conditions in the culture vessel

Solution

Question 6

A- Denaturation at a temperature of about 98$$^o$$C separating the two DNA strands.

A- Denaturation at a temperature of about 50$$^o$$C.

C- Extension in the presence of heat stable DNA polymerase.

B- Annealing with two sets of primers.

Solution

Question 7

Purification of the product

Ensuring anaerobic conditions in the culture vessel

Availability of oxygen throughout the process

Addition of preservatives to the product

Solution

Question 8

Prophase of mitosis

Prophase I of meiosis

Metaphase II of meiosis

Prophase II of meiosis

Solution

Question 9

Up stream processing

Screening

Down stream processing

Batch-culture

Solution

Question 10

Applied biology

Technology

Biotechnology

Biomedical engineering

Solution

User

Question Analysis

My Perfomance

Score

-

Rank

-

Results Announcement on: coming Monday

Stay Tuned: We’ll update you on your result via email or SMS.