Biotechnology Principles and Processes Test

Result

Biotechnology Principles and Processes Test - 29

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Weekly Quiz Competition

Question 1
1 / -0

Enzymes used in breaking DNA at specific sites are

A
DNA-ases

B
Endonucleases

C
Restriction endonucleases

D
Exonucleases

Solution

Restriction enzymes are used in genetic engineering for gene manipulation. It cuts DNA base pairs at specific sites. They cut the base pairs within the molecule so they are named as an endonuclease. 3000 restriction enzyme has been discovered till now. They are known as molecular scissors as they are used to cut a single gene from a larger piece of DNA. The restriction enzyme Eco RI, Bam II and Hind Ill are used in recombinant DNA technology to produce cuts in vector and other DNA molecules to obtain chimeric DNA.
So, the correct answer is option C.
Question 2
1 / -0

Branch of professional stream that uses living organisms or their products for obtaining useful substances at the molecular level is

A
Genetic engineering

B
Microbiology

C
Biotechnology

D
Olericulture

Solution
Question 3
1 / -0

Palindromic areas of DNA have

A
Repetitive sequences

B
Similar but opposite sequences in the two strands

C
Low melting

D
High melting

Solution

The two-strand of the DNA has complementary base-pair sequences. A palindromic sequence is the sequence of the DNA that reads the same from 5' to 3' on one strand and 5' to 3' on the complementary strand. It is also known as the inverted-reverse sequence.
So, the correct answer is option A.
Question 4
1 / -0

Which one of the following enzyme cuts the DNA within the specific positions?

A
Exonuclease

B
Alkaline phosphatase

C
Restriction endonuclease

D
Reverse transcriptase
Solution
- Restriction endonucleases recognize specific sequences in the DNA and cut the DNA into fragments.
- Each restriction endonuclease has a specific restriction site; cuts at specific sites produce uniform DNA fragments with sticky or blunts ends that are easily ligated with other DNA strands.
  Hence, the correct answer is C.
Question 5
1 / -0

Which of the following option is correct for recombinant DNA technology?

A
Endonuclease enzyme removes nucleotides from the ends of DNA

B
Exonuclease enzyme removes nucleotides from site within DNA

C
Endonuclease enzyme cut long polandric DNA strand

D
Exonuclease enzyme removes nucleotides from ends of DNA

Solution

Restriction endonucleases recognise specific sequences in the DNA and cut the DNA into fragments. The specific sites are 4-6 bp long and are called as restriction sites. Each restriction endonuclease has a specific restriction site. Exonuclease enzymes remove nucleotides one at a time from the end of a polynucleotide chain. They shorten the DNA by one nucleotide at a time, they do not digest it into fragments. Thus the correct answer is option D.
Question 6
1 / -0

Restriction enzymes are used to cut

A
Single stranded RNA

B
Double stranded DNA

C
Single stranded DNA

D
Double stranded RNA

Solution

Restriction enzymes are used in genetic engineering for gene manipulation. It cuts DNA base pairs at specific sites. They cut the base pairs within the molecule so they are named as an endonuclease. 3000 restriction enzyme has been discovered till now. They are known as molecular scissors as they are used to cut a single gene from a larger piece of double stranded DNA. The restriction enzyme Eco RI, Bam II and Hind Ill are used in recombinant DNA technology to produce cuts in vector and other DNA molecules to obtain chimeric DNA.
So, the correct answer is option B.
Question 7
1 / -0

In retroviruses, RNA dependent DNA polymerase synthesizes

A
DNA

B
RNA

C
RNA-DNA

D
None of the above

Solution

C. RNA-DNA
Solution : In retroviruses they can synthesize the DNA from RNA template in the host by help of reverse transcriptase that is RNA dependent DNA polymerase.
So the correct answer is " RNA-DNA"
Question 8
1 / -0

Transfer of DNA bands from agarose gel to nitrocellulose or nylon membrane is

A
Southern transfer

B
Western transfer

C
Northern transfer

D
Eastern transfer

E
Gene transfer

Solution

A. Southern Transfer
Solution : Southern Transfer is the process developed by Southern to transfer the DNA bands from agarose gel to nitrocellulose membrane in order to obtain a autoradiograph and visualize the bands under X ray.
So the correct answer is"Southern Transfer"
Question 9
1 / -0

Identify palindromic sequence

A
$$\frac{GAATTC}{CUUAAG}$$

B
$$\frac{GAATTC}{CTTUUG}$$

C
$$\frac{GAATTC}{CTTAAG}$$

D
$$\frac{GAATTC}{GAATIC}$$

Solution

C .GAATTC/CTTAAG
Solution : Palindromic sequence is the sequence of DNA bases which when read from the 5' end to 3' end is same when read from 3' end to 5 ' end.
So the correct answer is
GAATTC/CTTAAG
Question 10
1 / -0

Polymerase chain reaction is used for

A
In vivo replication

B
In vivo synthesis of mRNA

C
In vitro replication of DNA segment

D
In vitro synthesis of mRNA

Solution

C. In vitro replication of DNA segment
Solution : Polymerase chain reaction is the process by which the DNA segment is replicated from a small amount into a large number inside a PCR reactor.
So the correct answer is "In vitro replication of DNA segment"

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Re-Attempt Weekly Quiz Competition

Biotechnology Principles and Processes Test - 29

Result

Biotechnology Principles and Processes Test - 29

Score

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Rank

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SHARING IS CARING

Question 1

DNA-ases

Endonucleases

Restriction endonucleases

Exonucleases

Solution

Question 2

Genetic engineering

Microbiology

Biotechnology

Olericulture

Solution

Question 3

Repetitive sequences

Similar but opposite sequences in the two strands

Low melting

High melting

Solution

Question 4

Exonuclease

Alkaline phosphatase

Restriction endonuclease

Reverse transcriptase

Solution

Question 5

Endonuclease enzyme removes nucleotides from the ends of DNA

Exonuclease enzyme removes nucleotides from site within DNA

Endonuclease enzyme cut long polandric DNA strand

Exonuclease enzyme removes nucleotides from ends of DNA

Solution

Question 6

Single stranded RNA

Double stranded DNA

Single stranded DNA

Double stranded RNA

Solution

Question 7

DNA

RNA

RNA-DNA

None of the above

Solution

Question 8

Southern transfer

Western transfer

Northern transfer

Eastern transfer

Gene transfer

Solution

Question 9

$$\frac{GAATTC}{CUUAAG}$$

$$\frac{GAATTC}{CTTUUG}$$

$$\frac{GAATTC}{CTTAAG}$$

$$\frac{GAATTC}{GAATIC}$$

Solution

Question 10

In vivo replication

In vivo synthesis of mRNA

In vitro replication of DNA segment

In vitro synthesis of mRNA

Solution

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