Biotechnology Principles and Processes Test

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Biotechnology Principles and Processes Test - 34

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Weekly Quiz Competition

Question 1
1 / -0

Restriction endonucleases are called so as they

A
Synthesize DNA

B
Restrict nuclear activity

C
Cleave DNA into fragments

D
Break DNA at random

Solution

A restriction enzyme, restriction endonuclease is an enzyme that cleaves DNA into fragments at or near specific recognition sites within molecules known as restriction sites. Restriction enzymes are one class of the broader endonuclease group of enzymes.
So, option C is the correct option.
Question 2
1 / -0

Polymerase chain reaction is useful in _______.

A
DNA synthesis

B
DNA amplification

C
Protein synthesis

D
Amino acid synthesis
Question 3
1 / -0

Restriction endonucleases used widely in RDT are obtained from ____________.

A
Plasmids

B
Bacterial cells

C
Bacteriophages

D
All prokaryotic cells

Solution

Restriction endonucleases are called as molecular scissors. It is because of the special feature of these enzymes to cut at desired sites of DNA in the technique of genetic engineering. These are obtained from all prokaryotic cells.
So, option D is the correct option.
Question 4
1 / -0

Construction of first recombinant DNA was done by using plasmid of?

A
Salmonella typhimurium

B
Escherichia coli

C
Bacillus thuringiensis

D
Saccharomyces cerevisiae

E
Agrobacterium

Solution

Recombinant DNA or rDNA technology was discovered by Stanley Cohen and Herbert Boyer. They inserted the rDNA molecule they created into E.coli by means of a plasmid, thereby inducing the uptake and expression of a foreign DNA sequence also known as transformation.
So, option B is the correct option.
Question 5
1 / -0

Recombinant DNA or rDNA technology was discovered by

A
KhoranaBateson and de Vries

B
Sutton and Avery

C
Stanley Cohen and Herbert Boyer

D
Watson

Solution

Recombinant DNA or rDNA technology was discovered by Stanley Cohen and Herbert Boyer. They inserted the rDNA molecule they created into E.coli by means of a plasmid, thereby inducing the uptake and expression of a foreign DNA sequence also known as transformation.
So, option C is the correct option.
Question 6
1 / -0

Bacteria protect themselves from viruses by fragmenting viral DNA with

A
Endonuclease

B
Exonuclease

C
Gyrase

D
Ligase

Solution
Question 7
1 / -0

Gene amplification using primers can be done by

A
Microinjection

B
ELISA

C
Polymerase chain reaction

D
Gene gun

E
Electrophoresis

Solution

A primer is a short, single-stranded DNA sequence used in the polymerase chain reaction (PCR) technique. In the PCR method, a pair of primers is used to hybridize with the sample DNA and define the region of the DNA that will be amplified.
So the correct answer is option C.
Question 8
1 / -0

Which is correctly matched ________________.

A
Central dogma - Codon

B
RNA polymerase -RNA primer

C
Okazaki fragments - Splicing

D
Restriction enzyme - Genetic engineering

Solution

Restriction enzyme is an enzyme that is used in genetic engineering. Restriction endonuclease is also known a molecular scissors which cuts the DNA at a respective sides creating a sticky ends.
So, option D is the correct option.
Question 9
1 / -0

Restriction enzyme was discovered by

A
Alexander Fleming

B
Smith and Nathans

C
Berg

D
Waksman

Solution

Restriction enzyme, also called restriction endonucleases, a protein produced by bacteria that cleaves DNA at specific sites along the molecule.
These are discovered by Hamilton O. smith and Daniel Nathans in the late $$1960$$s and early $$1970$$s.
Question 10
1 / -0

Read statements a-d. Which of the following statements are incorrect?
a) First transgenic buffalo Rosie produced milk which was human alpha-lactalbumin enriched.
b) Restriction enzymes are used in isolation of DNA from other macromolecules.
c) Downstream processing is one of the steps of rDNA technology.
d) Disarmed pathogen vectors are also used in the transfer of rDNA into the host.

A
b and c

B
c and d

C
a and c

D
a and b

Solution

The first transgenic cow was developed in 1997, named Rosie. It consists of the human alpha-lactalbumin gene. Rosie was a cow, not buffalo, so the point a) is wrong.
Restriction enzymes can be isolated from bacterial cells and used in the laboratory to manipulate fragments of DNA by cutting them at interesting specific locations. Point b) is also wrong.
Hence the correct answer is option D.

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Re-Attempt Weekly Quiz Competition

Biotechnology Principles and Processes Test - 34

Result

Biotechnology Principles and Processes Test - 34

Score

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Rank

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SHARING IS CARING

Question 1

Synthesize DNA

Restrict nuclear activity

Cleave DNA into fragments

Break DNA at random

Solution

Question 2

DNA synthesis

DNA amplification

Protein synthesis

Amino acid synthesis

Question 3

Plasmids

Bacterial cells

Bacteriophages

All prokaryotic cells

Solution

Question 4

Salmonella typhimurium

Escherichia coli

Bacillus thuringiensis

Saccharomyces cerevisiae

Agrobacterium

Solution

Question 5

KhoranaBateson and de Vries

Sutton and Avery

Stanley Cohen and Herbert Boyer

Watson

Solution

Question 6

Endonuclease

Exonuclease

Gyrase

Ligase

Solution

Question 7

Microinjection

ELISA

Polymerase chain reaction

Gene gun

Electrophoresis

Solution

Question 8

Central dogma - Codon

RNA polymerase -RNA primer

Okazaki fragments - Splicing

Restriction enzyme - Genetic engineering

Solution

Question 9

Alexander Fleming

Smith and Nathans

Berg

Waksman

Solution

Question 10

b and c

c and d

a and c

a and b

Solution

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