Biotechnology Principles and Processes Test

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Biotechnology Principles and Processes Test - 39

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Weekly Quiz Competition

Question 1
1 / -0

Polymerase chain reaction

A
Is a method of synthesising human protein from human DNA

B
Uses restriction enzymes

C
Can produce billions of copies of a DNA fragment

D
Takes place naturally in bacteria

Solution

C. Can produce billions of copies of a DNA fragment
Solution : PCR or polymerase chain reaction is technique used in RDT to amplify or create a many copies of a gene in a very short time.
So the correct answer is "Can produce billions of copies of a DNA fragment"
Question 2
1 / -0

Isolation of DNA from a fungal cell involves the use of enzyme

A
Chitinase

B
Lysozyme

C
Eco RI

D
Hind II

Solution

Chitinase is an enzyme that degrades chitin, which is a chief constituent of fungal cell walls.
Question 3
1 / -0

Restriction endonucleases are enzymes that

A
Restrict the action of other enzymes

B
Break phosphodiester bond between specific nucleotides of DNA

C
Break nucleus into pieces

D
Break H-bond between two strands of DNA
Solution
Correct Option: B
Explanation:
Break the phosphodiester link between two DNA nucleotides.
Optional: Restriction endonuclease enzymes were developed from bacteria and function by cleaving the sugar phosphate link of the DNA at particular locations.
Hence, restriction endonuclease are enzymes that break phosphodiester bond between specific nucleotides of DNA.
Question 4
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Genes can be inserted into human cells by

A
PCR

B
Xenotransplantation

C
Modified viruses

D
Modified microarrays

Solution

C. Modified viruses
Solution : Modified viruses and their cos sites play a great role as vectors for inserting genes into cells. PCR is used for amplifying a small DNA fragment and microarrays are used for gene expression studies and xenotransplantation is grafting of new tissues into animals.
So the correct answer is " Modified viruses"
Question 5
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Which one of the following is NOT a PCR-based molecular marker?

A
RAPD

B
AP-PCR

C
DAF

D
RELP

Solution

D. RFLP
Solution : RFLP or Restriction Fragment Length Polymorphism is a molecular marker technique where the restriction sites of different enzymes in the same DNA sequence is mapped.Other marker techniques are all PCR assisted.
So the correct answer is " RFLP"
Question 6
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The plasmid of which organism was used successfully for the first time as vector by Cohen and

A
Boyer

B
Salmonella typhimurium

C
Streptococcus pneumoniae

D
Streptococcus aureus

E
Rhizobium leguminosarum

Solution

So the correct option is A.
Question 7
1 / -0

Restriction endonucleases are obtained from

A
Bacteria

B
All prokaryotic cells

C
Bacteriophages

D
Plasmids

Solution

So the correct option is A.
Question 8
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Which of the following is not component of downstream processing ____________.

A
Expression

B
Separation

C
Purification

D
Presentation
Question 9
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Find the correct statement with respect to function or properties of RNA polymerase.

A
Bacteria have $$3$$ different types of RNA polymerase

B
RNA polymerase binds to promoter and initiate transcription

C
It uses nucleoside monophosphate as substrate

D
RNA polymerase polymerizes in a template independent manner
Question 10
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Thermal cycler is used in one of these reactions.

A
Radioactivity

B
Enzyme catalysed reaction

C
Chemical reaction

D
Polymerase chain reaction

Solution

D. Polymerase Chain Reaction
Solution : Thermal cycler is used to heat the DNA to denature them and again renature them by reducing the temperature in a cyclic manner in order to amplify a small portion of DNA.
So the correct answer is "Polymerase Chain Reaction"

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Re-Attempt Weekly Quiz Competition

Biotechnology Principles and Processes Test - 39

Result

Biotechnology Principles and Processes Test - 39

Score

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SHARING IS CARING

Question 1

Is a method of synthesising human protein from human DNA

Uses restriction enzymes

Can produce billions of copies of a DNA fragment

Takes place naturally in bacteria

Solution

Question 2

Chitinase

Lysozyme

Eco RI

Hind II

Solution

Question 3

Restrict the action of other enzymes

Break phosphodiester bond between specific nucleotides of DNA

Break nucleus into pieces

Break H-bond between two strands of DNA

Solution

Question 4

PCR

Xenotransplantation

Modified viruses

Modified microarrays

Solution

Question 5

RAPD

AP-PCR

DAF

RELP

Solution

Question 6

Boyer

Salmonella typhimurium

Streptococcus pneumoniae

Streptococcus aureus

Rhizobium leguminosarum

Solution

Question 7

Bacteria

All prokaryotic cells

Bacteriophages

Plasmids

Solution

Question 8

Expression

Separation

Purification

Presentation

Question 9

Bacteria have $$3$$ different types of RNA polymerase

RNA polymerase binds to promoter and initiate transcription

It uses nucleoside monophosphate as substrate

RNA polymerase polymerizes in a template independent manner

Question 10

Radioactivity

Enzyme catalysed reaction

Chemical reaction

Polymerase chain reaction

Solution

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