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Biotechnology Principles and Processes Test - 52

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Biotechnology Principles and Processes Test - 52
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  • Question 1
    1 / -0
    Which of the following sequence is most likely to be a restriction enzyme recognization site ?
    Solution
    • Restriction enzymes are found in bacteria (and other prokaryotes). They recognize and bind to specific sequences of DNA, called restriction sites. Each restriction enzyme recognizes just one or a few restriction sites.
    • A palindromic sequence is a nucleic acid sequence in a double-stranded DNA or RNA molecule wherein reading in a certain direction on one strand matches the sequence reading in the same direction on the complementary strand.
    • most restriction enzymes recognize a "perfect" palindrome, Hence GTCGAC(the complimentary sequence is CAGCTG)  sequence is most likely to be a restriction enzyme recognization site,
    • So, the correct answer is "GTCGAC'.
  • Question 2
    1 / -0
    No. of restriction sites present in an ideal plasmid for each restriction enzyme.
  • Question 3
    1 / -0
    Restriction endonuclease enzyme obtained from?
  • Question 4
    1 / -0
    Bioreactors are useful in 

    Solution
    • Bioreactors are large culture vessels in which raw materials are biologically converted into specific products, using microbial, plant, animal or human cells or their enzymes. 
    • A bioreactor provides the optimal condition for achieving the desired product by providing optimum growth conditions (temperature, pH, substrate, salts, vitamins, oxygen, etc.) and helps in processing large volumes (100-1000 litres) of culture.
    So, the correct option is option B.
  • Question 5
    1 / -0
    Which steps are involved in genetically modifying an organism?
    A. Identification of desirable DNA
    B. Insertion of DNA into the host
    C. Maintenance of introduced DNA in the host
    D. Isolation of recombinant protein.
    Solution
    Identifying a trait of interest :- In order to identity a 
    desirable new wait scientists
    most often look to nature. successful discovered of a 
    new genetic trait is often a combination of critical 
    thinking and luck. 
    Insert the desired genetic Trait in to a new genome :-
    altering the genome of plant seeds is difficult due to 
    their rigid structure Many biotech companies use
    'gene gums' that shoot metal particles coated with 
    DNA into plant tissue.

  • Question 6
    1 / -0
    Which of the following is not required in PCR?
    Solution
    DNA primer is not required for a PCR reaction. 
    The reason for RNA primers to be used in PCR is the non availability of DNA primers. The RNA primers complimentary to the cellular DNA are easily synthesized by the DNA Primase enzyme which is nothing but RNA polymerase just like mRNA.
  • Question 7
    1 / -0
    Restriction endonucleases are extracted from:
    Solution
    Restriction endonuclease is a protein that is produced by bacteria which is used to cleave DNA at specific sites only. In the bacterial cell, they are produced to eliminate the foreign DNA thus eliminating the infecting organism. So the correct option is C. 
  • Question 8
    1 / -0
    The areas of application of PCR include:

    A. Production of monoclonal antibodies.
    B. Insertion of recombinant DNA into an organism.
    C. Diagnosis of a specific mutation.
    D. Detection of plant pathogens.
    Solution
    Answer is option C i.e. "C, D"

    PCR is used for diagnosis of diseases in animals and plants, it is also used to identify mutations in DNA. Major applications of PCR are:
    (1) Clinical Diagnosis
    (2) DNA Sequencing
    (3)  Gene Manipulation and Expression Studies
    (4) Forensic Medicine and (5) Gene Cloning.

    While, Production of monoclonal antibodies is done by Hybridoma technology and insertion of recombinant DNA into organisms is done by transformation or transduction.
  • Question 9
    1 / -0
    Which of the following is not a method of gene transfer in Bacteria?
    Solution
    There are three ways for the gene transfer in bacteria - conjugation , transformation, transduction. 
    But, Gametangia contact is the process in which fusion of the entire contents of two contacting gametangia which occur in fungi. So the correct option is D.
  • Question 10
    1 / -0
    Restriction enzymes are knowns as _____________.
    Solution
    Restriction enzymes are produced by bacterial cells that cleave DNA at specific sites. These enzymes are used to cleave foreign DNA and thus protecting the bacterium from infections. As they are involved in the cleavage of DNA, they are called molecular scissors. 
    So the correct option is B
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