Biotechnology Principles and Processes Test

Result

Biotechnology Principles and Processes Test - 54

Score
-
out of -
Rank
-
out of -

TIME Taken - -

SHARING IS CARING

If our Website helped you a little, then kindly spread our voice using Social Networks. Spread our word to your readers, friends, teachers, students & all those close ones who deserve to know what you know now.

Weekly Quiz Competition

Question 1

1 / -0

Which of the following should be chosen for best yield if one were to produce a recombinant protein in large amounts?

Laboratory flask of largest capacity

A stirred-tank bioreactor without in-lets and out-lets

A continuous bioreactor system

Any of the above

Solution

$$\textbf{Correct option:(C)}$$

$$\textbf{Explanation for correct option:}$$

$$\bullet$$ To produce large quantities of desired protein or product, bioreactors are used, these are large vessels (100-1000 liters) where large cultures are maintained.

$$\bullet$$ A bioreactor provides the optimal conditions for obtaining the desired product like optimal substrate, temperature, pH, vitamins, oxygen, and salts. Antibiotics and antifungals are used to maintain cultures free of any contamination.

$$\bullet$$ Bioreactors work on two types of technology:

$$\text{a)}$$ Batch system (closed system)

$$\text{b)}$$ Continuous culture system (open system)

$$\textbf{Batch system}$$	$$\textbf{Continuous culture system}$$
Everything needed is added in bioreactor like cells (containing the gene of interest), culture medium, and bioreactor is then operated for a set period of time. Nothing is further added.	In this technology, the used medium is drained out from one side (outlet) while a fresh medium is added from the other (inlet).
Metabolic waste accumulates in this system.	Metabolic waste does not accumulate in this system.
Cells cannot be maintained in the log/exponential phase all the time. Less product is obtained.	Cells are maintained in the log/exponential phase, which is a physiologically most active state and the maximum product is obtained (maximum yield).

Question 2
1 / -0

Which of the following is not a source of restriction endonuclease?

A
$$Haemophilus$$ $$influenzae$$

B
$$Escherichia$$ $$coli$$

C
$$Entamoeba$$ $$coli$$

D
$$Bacillius$$ $$amyloliquefaciens$$

Solution

$$\textbf{Correct option:(C)}$$

$$\textbf{Explanation for correct option:}$$

$$\bullet$$ $$Entamoeba$$ $$coli$$ is a non pathogenic species of $$Entamoeba$$, found in the gastro intestinal tract of humans. It is a protozoan and they are unicellular eukaryotic organisms.

$$\bullet$$ Restriction endonucleases are present only in prokaryotes (bacteria).

$$\textbf{Explanation for incorrect options:}$$

$$\bullet$$ $$Haemophilus$$ $$influenzae$$ is a source of Hind III.

$$\bullet$$ $$Escherichia$$ $$coli$$ is a source of Eco RI.

$$\bullet$$ $$Bacillius$$ $$amyloliquefaciens$$ is a source of Bam HI.

note : Eco RI , Bam HI and Hind III are restriction endonucleases.
Question 3
1 / -0

Which of the following has popularised the PCR (polymerase chain reactions)?

A
Fancy availability of DNA template

B
Availability of synthetic primers

C
Availability of cheap deoxyribonucleotides

D
Availability or Thermostable DNA polymerase

Solution

During in -vitro polymerisation of DNA, the reaction undergoes cycles of heating and cooling. The parent DNA is denatured at $$96^0C$$C. Then it is cooled to about $$55^0C$$ so that DNA primers can attach themselves to the DNA template. The extension of the primer takes place at $$72^0C$$ in presence of enzyme Taq polymerase which is a DNA polymerase that remains stable at this high temperature.
So, the correct answer is 'Availability or Thermostable DNA polymerase'
Question 4
1 / -0

While isolating DNA from bacteria, which of the following enzymes is not used?

A
Lysozyme

B
Ribonuclease

C
Deoxyribonuclease

D
Protease
Question 5
1 / -0

A cell is made competent to take up foreign DNA using the following methods except ?

A
A microinjection.

B
Biolistics

C
Bivalent $$Ca^{++}$$

D
Monovalent $$Na^+$$

Solution

A cell is made competent or enabled to take up a foreign for obtaining recombinant DNA using a microinjection (a glass pipette for transferring foreign DNA into the target cell. Biolistic method uses a gene gun to transfer DNA coated gold particle into the cell. Similarly use of divalent ion $$ Ca^{++}$$ also assists with uptake of foreign DNA by the cell.
So, the correct answer is 'Monovalent
Na+
Question 6
1 / -0

How many copies of the DNA sample are produced in PCR technique after 8-cycle?

A
356

B
456

C
256

D
128
Solution
(A) Correct answer C
(B) Explanation of correct answer:
Polymerase chain reaction (PCR) is a technique that exponentially amplifies a single copy of a specific DNA segment.
It generates thousands of copies of that specific DNA segment. After completion of one cycle, 2 copies are produced from a single DNA segment.
After completion of the second cycle, 2$$^2$$ = 4 copies are produced.
Similarly, after n$$^{th}$$ cycle, 2$$^2$$ copies are produced, where n is the number of cycles.
Hence, after the completion of 8 cycles, 2$$^8$$ = 256 copies will be produced.
Thus, the correct answer is '256.'
Question 7
1 / -0

Neomycin is extracted from

A
Streptomyces griseus

B
Streptomyces venezuelce

C
Streptomyces fradiae

D
None of the above

Solution

Streptomyces fradiae is a species of actinobacteria. It produces the antibiotics neomycin, tylosin and fosfomycin. Neomycin is an antibiotic which belongs to the group of aminoglycoside. The antibiotic is present in creams and ointments. It kills sensitive bacteria by stopping the production of essential proteins needed by the bacteria to survive.
Thus, the correct answer is option C.
Question 8
1 / -0

Woodruff (1941) was responsible for the isolation of

A
Neomycin

B
Actinomycin

C
Penicillin

D
Streptomycin

Solution

In 1940, Woodruff and Waksman isolated and purified actinomycin from Actinomyces griseus (later named Streptomyces griseus). Actinomycin is an antibiotic which inhibits the growth of gram-negative bacteria and thus, used to treat typhoid, dysentery, cholera, and undulant fever.
Question 9
1 / -0

A genetically engineered micro - organism used successfully in remeditation of oil spills is a species of

A
Pseudomonas

B
Trichoderma

C
Xanthomonas

D
Bacillus

Solution

Marine dwelling bacteria like Pseudomonas and fungi use the hydrocarbons as fuel and emit carbon dioxide as a result. The microbes break down the ring structures of the hydrocarbons in seaborne oil using enzymes and oxygen in the seawater. The end result is ancient oil turned into modern day bacterial biomass.
Question 10
1 / -0

Utility of fungi for steroid conversion was demonstrated by

A
Pasteur and Jaubert

B
Kohler and Milstein

C
Murray and Peterson

D
Waksman and Woodruff

Solution

The introduction of oxygen into a steroid molecule by means of Mucorales fungi has been described by Murray and Peterson. They suggested that Rhizopus stolonifer is capable of hydroxylation, necessary for steroid synthesis.

User

Question Analysis

Correct -
Wrong -
Skipped -

My Perfomance

Score
-
out of -
Rank
-
out of -

Re-Attempt Weekly Quiz Competition

Biotechnology Principles and Processes Test - 54

Result

Biotechnology Principles and Processes Test - 54

Score

-

Rank

-

SHARING IS CARING

Question 1

Laboratory flask of largest capacity

A stirred-tank bioreactor without in-lets and out-lets

A continuous bioreactor system

Any of the above

Solution

Question 2

$$Haemophilus$$ $$influenzae$$

$$Escherichia$$ $$coli$$

$$Entamoeba$$ $$coli$$

$$Bacillius$$ $$amyloliquefaciens$$

Solution

Question 3

Fancy availability of DNA template

Availability of synthetic primers

Availability of cheap deoxyribonucleotides

Availability or Thermostable DNA polymerase

Solution

Question 4

Lysozyme

Ribonuclease

Deoxyribonuclease

Protease

Question 5

A microinjection.

Biolistics

Bivalent $$Ca^{++}$$

Monovalent $$Na^+$$

Solution

Question 6

356

456

256

128

Solution

Question 7

Streptomyces griseus

Streptomyces venezuelce

Streptomyces fradiae

None of the above

Solution

Question 8

Neomycin

Actinomycin

Penicillin

Streptomycin

Solution

Question 9

Pseudomonas

Trichoderma

Xanthomonas

Bacillus

Solution

Question 10

Pasteur and Jaubert

Kohler and Milstein

Murray and Peterson

Waksman and Woodruff

Solution

User

Question Analysis

My Perfomance

Score

-

Rank

-

Results Announcement on: coming Monday

Stay Tuned: We’ll update you on your result via email or SMS.