Biotechnology Principles and Processes Test

Result

Biotechnology Principles and Processes Test - 57

Score
-
out of -
Rank
-
out of -

TIME Taken - -

SHARING IS CARING

If our Website helped you a little, then kindly spread our voice using Social Networks. Spread our word to your readers, friends, teachers, students & all those close ones who deserve to know what you know now.

Weekly Quiz Competition

Question 1
1 / -0

The sticky ends of a fragmented DNA molecule are made up of

A
Calcium salts

B
Endonuclease

C
Unpaired bases

D
Methyl groups
Solution
- The simplest DNA end of a double-stranded molecule is called a blunt end.
- In a blunt-ended molecule, both strands terminate in a base pair.
- Non-blunt ends are created by various overhangs.
- An overhang is a stretch of unpaired nucleotides at the end of a DNA molecule.
- These unpaired nucleotides can be in either strand, creating either 3' or 5' overhangs. Longer overhangs are called cohesive ends or sticky ends.
Therefore, the correct answer is option C.
Question 2
1 / -0

The natural function of restriction enzymes is to cut up foreign DNA. Who got the Nobel prize for their experiments on these enzymes?

A
W. Arber and H. Smith

B
D. Nathans

C
Both A and B

D
None of the above

Solution

Restriction endonuclease and its experiments were done by Arber, Smith, and Nathans who all were awarded the Nobel prize in 1978. They performed experiments proving that there are certain enzymes produced by the bacteria which help in the cleavage of the genomes of the pathogens.
So the correct answer is "Both A and B".
Question 3
1 / -0

The frequency of recombination is

A
Same for all genes

B
Highest for genes that first entered the recipient cells

C
Lowest for genes that first entered the recipient cells

D
None of the above

Solution

The frequency of recombinants determines the order of entry for each gene. During the process of conjugation, the genes are transferred through the conjugation tube. Spontaneous breakage of this tube creates a natural gradient of transfer. There are fewer chances that the latter genes will be received by the bacterial cell. The gene which enters the first in the cell has the maximum frequency of recombination compared to the other latter genes.
Thus, the correct answer is option B.
Question 4
1 / -0

Ideal host for the amplification of DNA molecules is

A
Viruses

B
Plants

C
Bacteria

D
Animals

Solution

Bacteria are ideal for the amplification of DNA because of 2 reasons; their short doubling time; and availability extensive knowledge and tools for manipulating the bacteria. The short doubling time is advantageous because it will provide enough amounts of amplified DNA (of our interest) in a short span of time. The fact that tools are available helps us to amplify a variety of DNA molecules.
So, the correct answer is 'Bacteria'
Question 5
1 / -0

PCR method is useful for

A
Amplification of DNA for forming billions of copies of itself.

B
Monoclonal antibody production.

C
Both of (A) and (B).

D
None of the above.

Solution

The polymerase chain reaction (PCR) is a technology in molecular biology used to amplify a single copy and to make multiple copies of the same fragment of the DNA. The two strands are separated and each strand is considered as the template strand for the synthesis of the new strand. In this way, many cycles are performed to produce multiple copies.
Thus, the correct answer is option A.
Question 6
1 / -0

Biohydrometallurgy is a/an

A
Deposition of minerals in soil by modified bacteria.

B
Production of metals by using bacteria.

C
The metals are deposited as insoluble oxides and sulphides due to large scale and long term activity of bacteria.

D
Association between biotechnology and metallurgy, creating a new discipline known as biohydrometallurgy to extract uranium and gold.

Solution

Biohydrometallurgy may be defined as that branch of biotechnology dealing with the study and application of the economic potential of the interactions between the microbial world and the mineral kingdom. Biohydrometallurgy is used to perform processes involving metals, for example, microbial mining, oil recovery, bioleaching, water-treatment and others. Biohydrometallurgy is mainly used to recover certain metals from sulfide ores. It is usually utilized when conventional mining procedures are too expensive or ineffective in recovering a metal such as copper, gold, lead, nickel and zinc.
Question 7
1 / -0

Sterilization of tissue culture medium is done by

A
Autoclaving of medium at 120$$^o$$ C for 15 minutes

B
Filtering the medium through fine sieve

C
Mixing the medium with antifungal agents

D
Keeping the medium at - 20$$^o$$C

Solution

Plant tissue culture media are generally sterilized by autoclaving at 120$$^o$$C and 1.05 kg/cm$$^2$$ (15-20 psi). The time required for sterilization depends upon the volume of medium in the vessel. The minimum times required for sterilization of different volumes of medium is 15 min.
So, the correct answer is 'Autoclaving of medium at 120
o C for 15 minutes'
Question 8
1 / -0

The biological enzymes used to synthesize DNA segments in retrovirus is

A
Reverse transcriptase

B
Restriction endonucleases

C
Restriction exonucleases

D
Restriction DNAase

Solution

A reverse transcriptase is an enzyme used to generate complementary DNA (cDNA) from an RNA template, in a process termed as reverse transcription. It is mainly associated with retroviruses.
Thus, the correct answer is option A.
Question 9
1 / -0

Restriction endonuclease cuts DNA at specific sites and cellular DNA is not damaged as

A
Rest enzyme susceptible sites are coated with protein

B
Rest enzyme susceptible sites are catalyzed by particular enzymes

C
They cleave DNA only at very limited and specific sites

D
All of the above

Solution

The restriction enzymes used in molecular biology usually recognize short target sequences of about 4 8 base pairs. Restriction enzymes are very specific to their cleavage function. The site which is recognized by this enzyme for cleavage is the restriction site. Typically, a restriction site will be a palindromic sequence of about four to six nucleotides long where restriction endonuclease makes nick.
Therefore, the correct answer is option C.
Question 10
1 / -0

Restriction enzymes are

A
Not always required in genetic engineering

B
Essential tools in genetic engineering

C
Nucleases that cleave DNA wherever it contains a particular short sequence of nucleotides

D
Both B and C

Solution

A restriction enzyme is an enzyme that cuts DNA at or near specific recognition nucleotide sequences known as restriction sites. These are the enzymes which are used in the genetic engineering techniques for the cleavage of the desired gene and the vector. Restriction enzymes recognize a sequence and produce a double-stranded cut in the DNA. All types of enzymes recognize specific short DNA sequences and carry out the endonucleolytic cleavage of DNA to give specific fragments with terminal 5'-phosphates.
Thus, the correct answer is option D.

User

Question Analysis

Correct -
Wrong -
Skipped -

My Perfomance

Score
-
out of -
Rank
-
out of -

Re-Attempt Weekly Quiz Competition

Biotechnology Principles and Processes Test - 57

Result

Biotechnology Principles and Processes Test - 57

Score

-

Rank

-

SHARING IS CARING

Question 1

Calcium salts

Endonuclease

Unpaired bases

Methyl groups

Solution

Question 2

W. Arber and H. Smith

D. Nathans

Both A and B

None of the above

Solution

Question 3

Same for all genes

Highest for genes that first entered the recipient cells

Lowest for genes that first entered the recipient cells

None of the above

Solution

Question 4

Viruses

Plants

Bacteria

Animals

Solution

Question 5

Amplification of DNA for forming billions of copies of itself.

Monoclonal antibody production.

Both of (A) and (B).

None of the above.

Solution

Question 6

Deposition of minerals in soil by modified bacteria.

Production of metals by using bacteria.

The metals are deposited as insoluble oxides and sulphides due to large scale and long term activity of bacteria.

Association between biotechnology and metallurgy, creating a new discipline known as biohydrometallurgy to extract uranium and gold.

Solution

Question 7

Autoclaving of medium at 120$$^o$$ C for 15 minutes

Filtering the medium through fine sieve

Mixing the medium with antifungal agents

Keeping the medium at - 20$$^o$$C

Solution

Question 8

Reverse transcriptase

Restriction endonucleases

Restriction exonucleases

Restriction DNAase

Solution

Question 9

Rest enzyme susceptible sites are coated with protein

Rest enzyme susceptible sites are catalyzed by particular enzymes

They cleave DNA only at very limited and specific sites

All of the above

Solution

Question 10

Not always required in genetic engineering

Essential tools in genetic engineering

Nucleases that cleave DNA wherever it contains a particular short sequence of nucleotides

Both B and C

Solution

User

Question Analysis

My Perfomance

Score

-

Rank

-

Results Announcement on: coming Monday

Stay Tuned: We’ll update you on your result via email or SMS.