Biotechnology Principles and Processes Test

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Biotechnology Principles and Processes Test - 68

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Question 1
1 / -0

Recognition site plasmid having 2 Kbp at 500 bp, 800 bp, 1300 bp, 2000 bp
How many bands are visualise after gel electrophoresis after treating with R.E.

A
2

B
3

C
1

D
4

Solution

As the restriction enzyme has a recognition site at 2Kb, after gel electrophoresis of the digested sample, two DNA fragments would be obtained.
So, the correct answer is '2'.
Question 2
1 / -0

Read the given statements and select the correct option.
Statement 1 : Restriction endonuclease enzymes recognise a specific palindromic nucleotide sequence in the DNA.
Statement 2 : Restriction endonuclease enzymes are called as molecular scissors or biological scissors.

A
Both statements 1 and 2 are correct

B
Statement 1 is correct but statement 2 is incorrect

C
Statement 1 is incorrect but statement 2 is correct

D
Both statements 1 and 2 are incorrect

Solution

Restriction endonuclease enzymes are also called molecular scissors which recognise a specific palindromic sequence in DNA chain. Example ECoRI.
So, the correct option is 'Option A' .
Question 3
1 / -0

The term 'chimeric DNA' refers to

A
DNA with overhanging stretches

B
DNA with palindromic sequence

C
A recombinant DNA

D
Molecular scissors

Solution

After cutting the source DNA and the vector DNA with a specific restriction enzyme, the cut out 'gene of interest' from the source DNA and the cut vector with space are mixed and ligase enzyme is added. This results in the formation of rDNA or hybrid DNA or chimeric DNA.
So, the correct answer is 'A recombinant DNA'.
Question 4
1 / -0

Study the following figures and identify the enzymes involved in steps I and II.

A
EcoRI and DNA Ligase

B
Hind II and DNA Ligase

C
EcoRI and HindII

D
Restriction endonuclease and exonuclease

Solution

At step I , there is a restriction endonuclease enzyme which works at the palindromic sequence AATTGC and at step 2, there is a DNA ligase which joins the separated fragments.
So, the correct option is 'Option A' .
Question 5
1 / -0

The term 'recombinant DNA' refers to

A
DNA of the host cell

B
DNA with a piece of foreign DNA

C
DNA with selectable marker

D
DNA with more than one recognition sites

Solution

After cutting the source DNA and the vector DNA with a specific restriction enzyme, the cut out 'gene of interest' from the source DNA and the cut vector with space are mixed and ligase enzyme is added. This results in the formation of rDNA or hybrid DNA or chimeric DNA.
So, the correct answer is 'DNA with a piece of foreign DNA'.
Question 6
1 / -0

Identify the palindromic sequence in the following.

A
$$\frac{GAATTC} {CTTUUG}$$

B
$$\frac{GGATCC} {CCTAGG}$$

C
$$\frac{CCTGG} {GGACC}$$

D
$$\frac{CGATA} {GCTAA}$$

Solution

The palindromes in DNA are base pair sequences that are the same when read forward (left to right) or backward (right to left) from a central axis of symmetry. Thus, $$\frac{GGATCC} {CCTAGG}$$ is a palindromic sequence.
Question 7
1 / -0

The first restriction endonuclease isolated was

A
EcoRI

B
BamHI

C
SalI

D
Hindll

Solution

The restriction enzymes cleave the DNA away from the recognition site. The first restriction endonuclease isolated was Hindll.
So, the correct option is 'Option D' .
Question 8
1 / -0

Which of the following contains the key tools for recombinant DNA technology?
(i) Restriction endonucleases, ligases, vectors(ii) Ligases, host organism, polymerase enzymes(iii) Vectors, Taq polymerase, primers(iv) Restriction exonucleases, ligases, primers, bioreactors

A
(i), (ii) and (iii)

B
(i) and (ii)

C
(i), (iii) and (iv)

D
(iii) and (iv)
Solution
Key tools for recombinant DNA technology:
Restriction endonuclease enzyme and DNA ligases for recombinant DNA production.
Vectors that help in carrying and integrating the desired gene.
Host organism is that one in which recombinant DNA is introduced.
Polymerase enzymes
So, the correct option is 'Option B' .
Question 9
1 / -0

The term 'chemical knife' refers to

A
Polymerases

B
Endonucleases

C
Ribonudeases

D
Cellulases

Solution

The restriction endonuclease enzyme inspects the length of a DNA sequence. Once it recognises specific sequence, it binds to the DNA and cuts each of the two strands of the double helix at specific points in their sugar phosphate back bone. Special sequence in the DNA recognised by restriction endonuclease is called palindromic nucleotide sequence. Restriction endonuclease enzymes are also known as molecular scissors or biological scissors or chemical knives or chemical scalpels.
So, the correct answer is 'Endonucleases'.
Question 10
1 / -0

The letter 'R' in EcoRI is derived from

A
The name of genus

B
The name of strain

C
The name of species

D
None of the above
Solution
In $$EcoRI$$,
$$E$$ comes from the genus Escherichia.
$$co$$ is from the species $$coli$$.
$$R$$ is from $$RY13$$ (strain).
The Roman number I indicates that it was the first enzyme isolated from the bacterium $$E.coli$$ $$RY$$ $$13$$.
So, the correct answer is 'The name of strain'.

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Biotechnology Principles and Processes Test - 68

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Biotechnology Principles and Processes Test - 68

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SHARING IS CARING

Question 1

2

3

1

4

Solution

Question 2

Both statements 1 and 2 are correct

Statement 1 is correct but statement 2 is incorrect

Statement 1 is incorrect but statement 2 is correct

Both statements 1 and 2 are incorrect

Solution

Question 3

DNA with overhanging stretches

DNA with palindromic sequence

A recombinant DNA

Molecular scissors

Solution

Question 4

EcoRI and DNA Ligase

Hind II and DNA Ligase

EcoRI and HindII

Restriction endonuclease and exonuclease

Solution

Question 5

DNA of the host cell

DNA with a piece of foreign DNA

DNA with selectable marker

DNA with more than one recognition sites

Solution

Question 6

$$\frac{GAATTC} {CTTUUG}$$

$$\frac{GGATCC} {CCTAGG}$$

$$\frac{CCTGG} {GGACC}$$

$$\frac{CGATA} {GCTAA}$$

Solution

Question 7

EcoRI

BamHI

SalI

Hindll

Solution

Question 8

(i), (ii) and (iii)

(i) and (ii)

(i), (iii) and (iv)

(iii) and (iv)

Solution

Question 9

Polymerases

Endonucleases

Ribonudeases

Cellulases

Solution

Question 10

The name of genus

The name of strain

The name of species

None of the above

Solution

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