Biotechnology Principles and Processes Test

Question 1
1 / -0

The given flow chart depicts the steps to transfer a desirable gene of interest into a plant.Isolation of desirable gene using restriction endonucleases and gel electrophoresis.
Regeneration of plants from the transformed cells to get transgenic plants.
Identify the missing steps (A, B and C) with regard to following statements and select the correct option.(i) Joining of desirable gene to a suitable cloning vector using ligases to create a recombinant DNA molecule(ii) Selection of transformed cells(iii) Transferring the recombinant DNA molecules to the target cells

A
A - i

B - ii

C - iii

B
A - i

B - iii

C - ii

C
A - ii

B - iii

C - i

D
A - iii

B - i

C - ii

Question 2
1 / -0

In a polymerase chain reaction after the denaturation step why the mixture needs to cool down to a lower temperature?

A
To permit specific annealing of the primers

B
To give a halt to the reaction mixture

C
To increase the activity of enzyme Taq polymerase

D
To obtain the multiple copies of the DNA

Question 3
1 / -0

Identify the figures (A) and (B) and select the correct option.

A
A - Sparged stirred-tank bioreactor, B- Simple stirred-tank bioreactor

B
A - Sparged stirred-tank bioreactor, B - Sparged stirred-tank bioreactor

C
A - Simple stirred-tank bioreactor, B- Sparged stirred-tank bioreactor

D
A- Simple stirred-tank bioreactor, B- Simple stirred-tank bioreactor

Question 4
1 / -0

How many fragments will be generated if you digest a linear DNA molecule with a restriction enzyme having four recognition sites on the DNA?

A
3

B
6

C
5

D
4

Question 5
1 / -0

Which of the following steps should be performed by a person in order to visualise the bands of DNA fragments obtained from gel electrophoresis?

A
Exposure of DNA fragments to UV radiations

B
Staining with bromophenol blue followed by exposure to UV radiations

C
Staining with ethidium bromide followed by exposure to UV radiations

D
Person can see the bands without staining

Question 6
1 / -0

Primers are

A
Chemically synthesised oligonucleotides that are complementary to the regions of DNA

B
Chemically synthesised oligonucleotides that are not complementary to the regions of DNA

C
Chemically synthesised, autonomously replicating circular DNA molecules

D
Specific sequences present on recombinant DNA

Question 7
1 / -0

Study the following statements regarding recombinant DNA technology and select the incorrect ones
(i) Taq polymerase extends the primers using the nucleotides provided in the reaction
(ii) Antibiotic resistance genes are considered as desirable genes in recombinant DNA technology
(iii) DNA fragments are separated according to their charge only, in agarose gel electrophoresis
(iv) Transformation is a procedure through which a piece of DNA is integrated into the genome of a host bacterium
(v) To produce higher yields of the desired protein, host cells can be multiplied in a continuous culture
(vi) Downstream processing is one of the steps of polymerase chain reaction

A
(i), (iii) and (vi)

B
(i), (iii) and (v)

C
(ii), (iii) and (v)

D
(i), (iv) and (v)

Question 8

1 / -0

Given figures represent the steps involved in polymerase chain reaction (PCR). Identify the steps A, B, C and D, and select the correct option.

	A	B	C	D
a	Denaturation at 94-96 degree C	Extension through Taq polymerase at 72 degree C	Repetition of denaturation and annealing	Annealing at 72 degree C
b	Denaturation at 94-96 degree C	Annealing at 40-60 degree C	Extension through Taq polymerase at 72 degree C	Repetition of denaturation and annealing
c	Denaturation at 40-60 degree C	Annealing at 72 degree C	Extension through Taq polymerase at 94-96 degree C	Repetition of denaturation and annealing
d	Extension through Taq polymerase at 72 degree C	Denaturation at 40 degree C	Annealing at 72 degree C	Repetition of denaturation and annealing

a

b

c

d

Question 9

1 / -0

Match column I with column II and select the correct option from the codes given below.

	Column I		Column II
A.	Exonucleases	(i)	Stable above $$90^oC$$
B.	Polynucleotide kinases	(ii)	Cleave the end of linear DNA
C.	Taq DNA polyrnerase	(iii)	Add phosphate to 5' OH end
D.	Terminal transferases	(iv)	add a number of nucleotides to 3' end of DNA or RNA
		(v)	Regulate the level of supercoiling of DNA molecule

A - (ii), B - (iii), C - (i), D - (iv)

A - (ii), B - (v), C - (i), D - (iii)

A - (ii), B - (iv), C - (i), D - (v)

A - (iii), B - (iv), C - (i), D - (ii)

Question 10
1 / -0

Which of the following DNA sequences qualifies to be designated as a palindrome?

A
5' - GACCAG - 3' in one strand

B
3' - GACCAG - 5' in one strand

C
5' - GACGA.G - 3'

3' - CTGGTC - 5'

D
5' - AGCGCT - 3'

3' - TCGCGA - 5'

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Biotechnology P...

Question 1

A - iB - iiC - iii

A - iB - iiiC - ii

A - iiB - iiiC - i

A - iiiB - iC - ii

Question 2

To permit specific annealing of the primers

To give a halt to the reaction mixture

To increase the activity of enzyme Taq polymerase

To obtain the multiple copies of the DNA

Question 3

A - Sparged stirred-tank bioreactor, B- Simple stirred-tank bioreactor

A - Sparged stirred-tank bioreactor, B - Sparged stirred-tank bioreactor

A - Simple stirred-tank bioreactor, B- Sparged stirred-tank bioreactor

A- Simple stirred-tank bioreactor, B- Simple stirred-tank bioreactor

Question 4

3

6

5

4

Question 5

Exposure of DNA fragments to UV radiations

Staining with bromophenol blue followed by exposure to UV radiations

Staining with ethidium bromide followed by exposure to UV radiations

Person can see the bands without staining

Question 6

Chemically synthesised oligonucleotides that are complementary to the regions of DNA

Chemically synthesised oligonucleotides that are not complementary to the regions of DNA

Chemically synthesised, autonomously replicating circular DNA molecules

Specific sequences present on recombinant DNA

Question 7

(i), (iii) and (vi)

(i), (iii) and (v)

(ii), (iii) and (v)

(i), (iv) and (v)

Question 8

a

b

c

d

Question 9

A - (ii), B - (iii), C - (i), D - (iv)

A - (ii), B - (v), C - (i), D - (iii)

A - (ii), B - (iv), C - (i), D - (v)

A - (iii), B - (iv), C - (i), D - (ii)

Question 10

5' - GACCAG - 3' in one strand

3' - GACCAG - 5' in one strand

5' - GACGA.G - 3'3' - CTGGTC - 5'

5' - AGCGCT - 3'3' - TCGCGA - 5'

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A - i

B - ii

C - iii

A - i

B - iii

C - ii

A - ii

B - iii

C - i

A - iii

B - i

C - ii

5' - GACGA.G - 3'

3' - CTGGTC - 5'

5' - AGCGCT - 3'

3' - TCGCGA - 5'