Biotechnology Test

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Biotechnology Test - 3

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Weekly Quiz Competition

Question 1
1 / -0

EtBr fluoresces at the wavelength

A
400 nm

B
254 nm

C
500 nm

D
340 nm

Solution

Ethidium bromide is an intercalating agent commonly used as a fluorescent tag (nucleic acid stain) in molecular biology laboratories for techniques such as agarose gel electrophoresis. It is commonly abbreviated as "EtBr", which is also an abbreviation for bromoethane. When exposed to ultraviolet light, it will fluoresce with an orange colour, intensifying almost 20-fold after binding to DNA. It fluoresces at the wavelength at 254 nm.
Question 2
1 / -0

OCT, XAM and CAM are

A
plasmids used in DNA probe

B
plasmids helpful in bioremediation

C
plasmids used to produce disease-resistant plants

D
plasmids to promote conjugation

E
None of these
Question 3
1 / -0

Which of the following statements is true about complementary DNA (cDNA)?

A
It is the "other strand" of a DNA.

B
It does not need any template for its synthesis.

C
It is the base sequence of the vector DNA.

D
It is produced by reverse transcription.
Question 4
1 / -0

Directions: The following question has four choices out of which ONLY ONE is correct.

What is antisense technology?

A
RNA polymerase producing DNA

B
A cell displaying a foreign antigen used for synthesis of antigens

C
Production of somaclonal variants in tissue cultures

D
When a piece of RNA that is complementary in sequence is used to stop expression of a specific gene
Question 5
1 / -0

Shotgun technique is employed in

A
stitching of genes

B
isolation of genes

C
splicing of DNA

D
duplicating genomic DNA
Question 6
1 / -0

Protein encoding gene expressed in heterologous host is called as

A
Peptones

B
Tertiary proteins

C
Recombinant protein

D
Selectable marker

Solution

Recombinant Protein is a protein encoded by a gene — recombinant DNA — that has been cloned in a system that supports expression of the gene and translation of messenger RNA (see expression system). Modification of the gene by recombinant DNA technology can lead to expression of a mutant protein. Protein encoding genes are commonly described as structural or regulatory. Structural gene encodes enzymes and structural protein.
Question 7
1 / -0

The enzyme used to cut the DNA molecule at specific site is

A
restriction endonuclease

B
DNA-ligase

C
DNA-polymerase

D
RNA-polymerases
Question 8
1 / -0

The techniques of using live organisms of enzymes from organism to produce products and processes useful to human is called

A
Biotechnology

B
Mutation

C
Regeneration

D
Hybridization

Solution

Biotechnology is the use of living systems and organisms to develop or make products, or "any technological application that uses biological systems, living organisms, or derivatives thereof, to make or modify products or processes for specific use".
Question 9
1 / -0

PCR based markers have been developed to

A
create terminate codon

B
manufacture antibiotics

C
differentiate different biotypes of gall midge

D
obtain soil microorganisms with the help of specific enzyme reductase
Question 10
1 / -0

Yeast-two hybrid system is used to study

A
yeast replication

B
yeast protein synthesis

C
protein-protein interaction

D
transcription
Question 11
1 / -0

Kohler and Milstein developed the technique for

A
production of monoclonal antibodies

B
steroid synthesis

C
immobilisation of enzymes

D
C-DNA library

Question 12

1 / -0

Match the biologists given in Column I with their specific fields of achievements given in Column II.

Column I	Column II
A. Alec Jefferey	i. Cytogenetics and radiation genetics in plants
B. M. S. Swaminathan	ii. Function of DNA ligase
C. Eli Lily	iii. DNA finger printing technology
D. Mertz and Devis	iv. Preparation of humulin

A – ii, B – i, C – iv, D – iii

A – iii, B – i, C – iv, D – ii

A – iv, B – iii, C – ii, D – i

A – ii, B – iv, C – i, D – iii

Question 13
1 / -0

Directions: Following question consists of two statements, one labelled as 'Assertion A' and the other labelled as 'Reason R'. You are to examine these statements carefully and decide if the Assertion (A) and the Reason (R) are individually true and if so, whether the Reason is a correct explanation for Assertion. Select your answer from the codes given below.

(A) : DNA ligase is used as a fusogenic agent in protoplasmic fusion technology.
(R) : Fusogenic agent will promote the fusion of protoplasts.

A
Both (A) and (R) are true and (R) is the correct explanation of (A).

B
Both (A) and (R) are true and (R) is not the correct explanation of (A).

C
(A) is true, but (R) is false.

D
(A) is false, but (R) is true.
Question 14
1 / -0

Consider the following statements:

(1) BT cotton is a hybrid plant.
(2) National Research Centre on Plant Biotechnology is located in New Delhi.

Which of the given statements is/are correct?

A
Only (1)

B
Only (2)

C
Both (1) and (2)

D
Neither (1) nor (2)
Question 15
1 / -0

Brief exposure of cells to an electric field also allow the bacteria to take up DNA and this process is called as

A
Electroporation

B
Electrolysis

C
Electrophoresis

D
Apheresis

Solution

Electroporation, or electropermeabilization, is a microbiology technique in which an electrical field is applied to cells in order to increase the permeability of the cell membrane, allowing chemicals, drugs, or DNA to be introduced into the cell
Question 16
1 / -0

A radioisotope used to label proteins differently from nucleic acids is

A
14C

B
15N

C
32P

D
35S
Question 17
1 / -0

Hypervariables are useful in

A
DNA fingerprinting

B
genetic engineering

C
cloning

D
gene sharing
Question 18
1 / -0

The mass of undifferentiated cells is called

A
callus

B
explant

C
suspension culture

D
leaven
Question 19
1 / -0

A transgene can

A
prevent the expression of a native gene

B
modify an existing biosynthetic pathway

C
produce a protein that itself produces a phenotype of interest

D
All of the above
Question 20
1 / -0

Host cell membrane can be made permeable by

A
Zinc

B
CaCl₂

C
Ammonium Carbonate

D
Tollen’s reagent

Solution

CaCl₂ can be used to make the bacterial cell a permeable cell, and by introducing these cells (after CaCl₂ being placed into the mixture of bacterial cells), a plasmid for ampicillin resistance can be transformed into the bacteria. The standard method for making the bacteria permeable to DNA involves treatment with calcium ions

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Biotechnology Test - 3

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Biotechnology Test - 3

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Question 1

400 nm

254 nm

500 nm

340 nm

Solution

Question 2

plasmids used in DNA probe

plasmids helpful in bioremediation

plasmids used to produce disease-resistant plants

plasmids to promote conjugation

None of these

Question 3

It is the "other strand" of a DNA.

It does not need any template for its synthesis.

It is the base sequence of the vector DNA.

It is produced by reverse transcription.

Question 4

RNA polymerase producing DNA

A cell displaying a foreign antigen used for synthesis of antigens

Production of somaclonal variants in tissue cultures

When a piece of RNA that is complementary in sequence is used to stop expression of a specific gene

Question 5

stitching of genes

isolation of genes

splicing of DNA

duplicating genomic DNA

Question 6

Peptones

Tertiary proteins

Recombinant protein

Selectable marker

Solution

Question 7

restriction endonuclease

DNA-ligase

DNA-polymerase

RNA-polymerases

Question 8

Biotechnology

Mutation

Regeneration

Hybridization

Solution

Question 9

create terminate codon

manufacture antibiotics

differentiate different biotypes of gall midge

obtain soil microorganisms with the help of specific enzyme reductase

Question 10

yeast replication

yeast protein synthesis

protein-protein interaction

transcription

Question 11

production of monoclonal antibodies

steroid synthesis

immobilisation of enzymes

C-DNA library

Question 12

A – ii, B – i, C – iv, D – iii

A – iii, B – i, C – iv, D – ii

A – iv, B – iii, C – ii, D – i

A – ii, B – iv, C – i, D – iii

Question 13

Both (A) and (R) are true and (R) is the correct explanation of (A).

Both (A) and (R) are true and (R) is not the correct explanation of (A).

(A) is true, but (R) is false.

(A) is false, but (R) is true.

Question 14

Only (1)

Only (2)

CaCl₂