Biotechnology: ...

The techniques of using live organisms of enzymes from organism to produce products and processes useful to human is called?

Protein encoding gene expressed in heterologous host is called as______.

Host cell membrane can be made permeable by

Brief exposure of cells to an electric field also allow the bacteria to take up DNA and this process is called as_______.

EtBr fluoresces at the wavelength

Phage is a______.

Plasmid is a________.

The technique used to produce large number of genetically identical offspring is called as______.

Electroporation facilitates introduction of foreign DNA into the target organism by

Restriction enzymes belongs to a larger class of enzymes called?

The extraction of DNA from the agrose gel is called as______.

Proteins can be digested by

The nuclease will act on______.

Eco RI acting on goi and vector will produce?

The polyacrylamide gel is used to separate proteins as________.

Primers are______.

The separation and purification of recombinant protein product is called as______.

The smallest bands in the agarose gel will be towards

Which of the following contributed in popularising the PCR (polymerase chain reactions) technique?

Helium is used in Biolistic gun as______.

Thermostable DNA polymerase is isolated from bacterium______.

What modification in the Ti plasmid of Agrobacterium tumifaciens is done to use it as a cloning vector which is not pathogenic?

Which of the following is correct sequence of process of recombinant DNA technology-
i. Isolation of DNA
ii. Isolation of desired DNA fragment.
iii. Fragmentation of DNA by restriction endonuclease.
iv. Transferring of into host
v. Ligation of DNA fragment into vector
vi. Culturing in host cell to get desired product.

The sequence of the 5’ and 3’ primers for the following DNA sequence will be:
5’ AATGCGGCAATCGAGTC3’
3’ TTACGCCGTTAGCTCCAG5’

The agarose gel with high % of agar will lead to accumulation of large sized DNA fragment near the wells as: