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Biotechnology Principles and Processes Test - 41

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Biotechnology Principles and Processes Test - 41
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  • Question 1
    1 / -0
    Which one of the following is the method of gene transfer based on the use of short electrical impulses of high field strength?
    Solution
    B. Electroporation
    Solution : Electroporation is the use of short impulses of electricity to increase the permeability of cells in order to introduce the target DNA segment inside it.
    So the correct answer is " Electroporation"
  • Question 2
    1 / -0
    Consider the following steps during recombinant DNA technology.
    a. Breaking of donor DNA using restriction enzyme
    b. Culture of cloned bacteria containing the fragment of donor DNA
    c. Isolation of suitable plasmid
    d. Insertion of recombinant DNA through cloning
    e. Joining of donor DNA with a suitable plasmid using ligase
    The correct sequence of these is?
    Solution
    C.1,3,5,4,2
    Solution : In a recombinant DNA technology method first we break the donor DNA at specific site by restriction endonuclease. Then we isolate a suitable plasmid from a bacteria and join the DNA with the plasmid to create recombinant DNA. This DNA is inserted into a bacteria or a cell and cultured .
    So the correct answer is "1,3,5,4,2"
  • Question 3
    1 / -0
    Which one of the following enzymes is used in PCR reaction?
    Solution
    A. Taq Polymerase
    Solution : Taq polymerase is a high temperature tolerant polymerase that is used in PCR. It is isolated from thermus acquaticus( thermophile) and is used in PCR where DNA polymerization occurs in high temperatures.
    So the correct answer is "Taq polymerase"
  • Question 4
    1 / -0
    The most commonly used method of choice for gene transfer using immature embryos as explants is ______________.
    Solution
    A. Microprojectile
    Solution : Gene transfer using immature embryos as explants mainly uses the microprojectile method or high speed particle bombardment. This is most successful because it is independent of any vector,cell type or the species of the organism.
    So the correct answer is " Microprojectile"
  • Question 5
    1 / -0
    When E coli with DNA $$^{15}N ^{15}N $$ is allowed to grow in a medium containing $$^{14}N$$ the number of DNA strands with $$^{15}N$$ after five generations is: 
  • Question 6
    1 / -0
    How many cycles of PCR are required if desired number of DNA molecules is 64 and starting material is single stranded DNA instead of ds DNA ?
  • Question 7
    1 / -0
    Which of the following techniques can be used to detect genetic disorders in humans?
    Solution
    PCR: Polymerase Chain Reaction is a technique that can amplify a target DNA segment. This amplified segment aids in the detection of various genetic disorder and further studies.
    Gel Electrophoresis: It is a method used for separation of macro molecules (DNA, RNA, Proteins) on the basis of their charge:mass ratio.
    Chromatography: It is a separation technique that uses stationary phase and mobile phase to isolate pure compound/s from a mixture.
    Spectroscopy: It is the study of the interaction of light with matter. For example absorption or emission of light and other radiations by mass. Spectroscopy can be used to identify the structure or functional groups of organic compounds. So, the correct answer is option A
  • Question 8
    1 / -0
    Which one of the following is not essential for polymerase chain reaction (PCR)?
    Solution
    Polymerase chain reaction (PCR) is a technique used to amplify a single copy of desired segment of DNA up to several orders of magnitude. The process requires thermostable DNA polymerase (Taq polymerase), Specifically designed DNA primers and nucleotides. Each cycle consists of three steps, denaturation, annealing and extension.
    Thus, the correct answer is option A.

  • Question 9
    1 / -0
    If the initial number of template DNA molecules in a PCR reaction is $$1000$$, the number of product DNA molecules at the end of $$20$$ cycles will be closest to.
  • Question 10
    1 / -0
    One strand of the given DNA segment codes for mRNA having the sequence AUC GCG UCA for the synthesis of protein. Which DNA strand is responsible for the above mRNA sequence ________________.
    Solution
    As we know DNA contain Adenine, Thymine, Guanine, Cytosine where A binds with T and G binds with C by hydrogen bonds while in case of RNA only Uracil replaces Thymine which A binds with U and G binds with C.
    So, the correct option is option B.
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