Biotechnology P...

Which one of the following is the method of gene transfer based on the use of short electrical impulses of high field strength?

Consider the following steps during recombinant DNA technology.
a. Breaking of donor DNA using restriction enzyme
b. Culture of cloned bacteria containing the fragment of donor DNA
c. Isolation of suitable plasmid
d. Insertion of recombinant DNA through cloning
e. Joining of donor DNA with a suitable plasmid using ligase
The correct sequence of these is?

Which one of the following enzymes is used in PCR reaction?

The most commonly used method of choice for gene transfer using immature embryos as explants is ______________.

When E coli with DNA $$^{15}N ^{15}N $$ is allowed to grow in a medium containing $$^{14}N$$ the number of DNA strands with $$^{15}N$$ after five generations is: 

How many cycles of PCR are required if desired number of DNA molecules is 64 and starting material is single stranded DNA instead of ds DNA ?

Which of the following techniques can be used to detect genetic disorders in humans?

Which one of the following is not essential for polymerase chain reaction (PCR)?

If the initial number of template DNA molecules in a PCR reaction is $$1000$$, the number of product DNA molecules at the end of $$20$$ cycles will be closest to.

One strand of the given DNA segment codes for mRNA having the sequence AUC GCG UCA for the synthesis of protein. Which DNA strand is responsible for the above mRNA sequence ________________.