Biotechnology Principles and Processes Test

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Biotechnology Principles and Processes Test - 50

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Weekly Quiz Competition

Question 1
1 / -0

Batch Fermentation:

A
Is a type of closed system

B
Utilizes continuous draining of used medium from one side and adding fresh medium from other to maintain the cells in their physiologically most active log exponential phase

C
Maintain the cells in log phase

D
Is same as continuous culture system

Solution

Batch fermentation is a type of closed-culture fermentation where the sterile nutrient medium is taken and inoculated with appropriate microorganisms and is left for a certain period of time to allow growth in the fermentor. Nothing is added or removed from the fermentor during the process. The product is separated at the end.

So, the correct answer is 'Is a type of closed system'.
Question 2
1 / -0

If we want more copies of target (desired) DNA then it should be cloned in a vector having ___________________.

A
Large number of selectable markers

B
Large number of cloning sites

C
Many antibiotic resistance genes

D
Ori which supports high copy number
Question 3
1 / -0

How many fragments will be generated in total upon digestion of a closed circular DNA molecule and liner DNA molecule with a same restriction enzyme having six recognition sites on both DNA molecules?

A
$$13$$

B
$$14$$

C
$$12$$

D
$$6$$

Solution

Restriction endonucleases are a class of enzymes that cut the DNA by recognising a specific sequence or base pairing of the DNA.
These enzymes have a very important role in recombinant DNA techniques.
Linear DNA is the form of DNA present in the eukaryotic nucleus and is composed of two free ends. Circular DNA is predominantly found in prokaryotes, whereas the mitochondria, chloroplast and plasmids also contain circular DNA. Circular DNA is found in the cytoplasm of the prokaryotic cell, in mitochondria or in the chloroplast.
So when the restriction endonuclease will cut the DNA from the recognition site fragments of DNA will be produced. As the linear NA has 2 free ends it will produce 7 fragments and the circular DNA will produce 6 fragments.

So, the correct option is '13'.
Question 4
1 / -0

Identify the correct sequence of steps in the construction of recombinant DNA.
$$(1)$$ use restriction enzymes
$$(2)$$ use DNA ligase
$$(3)$$ remove plasmid from parent bacterium
$$(4)$$ introduce plasmid into new host bacterium.

A
$$1-2-3-4$$

B
$$4-3-2-1$$

C
$$3-1-2-4$$

D
$$2-3-1-4$$

Solution

Generation of rDNA steps:
1) The DNA for insulin is first disengaged.
2) A plasmid made of DNA is expelled from a bacterial cell.
3) A limitation protein cuts the plasmid DNA open, leaving sticky finishes.
4) The insulin quality, with integral sticky closures, is included.
5) DNA ligase catalyst grafts (joins) together with the plasmid DNA and the Insulin DNA.
6) The plasmid (now hereditarily changed) is embedded again into the bacterium.
7) The bacterium has cell, partitions and delivers duplicates of the plasmid.
8) The Bacterium makes human insulin utilizing the quality in the plasmid.
9) The insulin is removed from the bacterial culture.
So, the correct option '2−3−1−4'.
Question 5
1 / -0

Sparger in a bioreactor facilities

A
Purification of product

B
Maintenance of temperature and pH control

C
Availability of oxygen through the process

D
Maintenance of anaerobic conditions

Solution

Bioreactor refers to any manufactured or engineered device or system that supports a biologically active environment. In aerobic cultivation process, the purpose of the sparger is to supply adequate oxygen to the growing cells.
So, the correct option is 'Availability of oxygen through the process'.
Question 6
1 / -0

E.coli cloning vector pBR322 showing restriction sites, ori and antibiotic resistance genes is given below. Select the correct option.

A
A-Hind III,B-Eco RI, C-Bam H I, D-Sal I

B
A-Eco RI, B-Hind III, C-Bam HI, D-Sal I

C
A-Eco RI, B-BamH I, C-Hind III, D-Sal I

D
None of these

Solution

The correct answer is 'A-Eco RI, B- Hind III, C- Bam HI, D-Sal I'.
Option B is correct.
Question 7
1 / -0

Column I Column II
I. Agarose A. PCR
II. Opines B. Gene gun
III. Biolistic C. Ti plasmid
iv. Thermal cycler D. Sea weeds

A
$$I-D,II-A,III-B,IV-C$$

B
$$I-D,II-C,III-B,IV-A$$

C
$$I-D,II-A,III-C,IV-B$$

D
$$I-A,II-D,III-B,IV-C$$
Solution
- Agarose is derived from seaweeds Gelidium and Gracilaria
- Opine synthesis is the function of Ti plasmid of Agrobacterium
- Biolistic is the term used for gene gun used for biotransformation
- Thermocycler is used to carry out PCR reaction
So the correct option is B. (I−D,II−C,III−B,IV−A)
Question 8
1 / -0

Which of the following techniques can be used to introduce foreign DNA into the cell?

A
Using disarmed pathogen

B
Microinjection

C
Gene gun

D
All of these

Solution

Vectors, gene gun ( biolistic ) and microinjection are all viable techniques to insert foreign DNA into the host cell. So, the correct option is All of these.
Question 9
1 / -0

Insertional inactivation is related to

A
Microinjection

B
Gene gun

C
Gel electrophoresis

D
Selection of recombinations

Solution

Insertional inactivation is the inactivation of a gene upon insertion of another gene inside in its place or within its coding sequence. This helps the selection of transformant and as well as recombinant colonies in selective antibiotic medium aided due to insertional inactivation of the antibiotic gene which becomes inactive due to foreign gene insert within its sequence.
So, the correct option is Selection of recombination.
Question 10
1 / -0

The areas of application of PCR include

A. Production of monoclonal antibodies
B. Insertion of recombinant DNA into an organism
C. Diagnosis of specific mutation
D. Detection of plant pathogens

A
A, B

B
B, C

C
C, D

D
A, B, C, D
Solution
The areas of application of PCR include
Clinical Diagnosis
DNA Sequencing
Gene Manipulation and Expression Studies
in Comparative Studies of Genomes
in Forensic Medicine
in Comparison with Gene Cloning
So, the correct option is 'C,D'.

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Re-Attempt Weekly Quiz Competition

Biotechnology Principles and Processes Test - 50

Result

Biotechnology Principles and Processes Test - 50

Score

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Rank

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SHARING IS CARING

Question 1

Is a type of closed system

Utilizes continuous draining of used medium from one side and adding fresh medium from other to maintain the cells in their physiologically most active log exponential phase

Maintain the cells in log phase

Is same as continuous culture system

Solution

Question 2

Large number of selectable markers

Large number of cloning sites

Many antibiotic resistance genes

Ori which supports high copy number

Question 3

$$13$$

$$14$$

$$12$$

$$6$$

Solution

Question 4

$$1-2-3-4$$

$$4-3-2-1$$

$$3-1-2-4$$

$$2-3-1-4$$

Solution

Question 5

Purification of product

Maintenance of temperature and pH control

Availability of oxygen through the process

Maintenance of anaerobic conditions

Solution

Question 6

A-Hind III,B-Eco RI, C-Bam H I, D-Sal I

A-Eco RI, B-Hind III, C-Bam HI, D-Sal I

A-Eco RI, B-BamH I, C-Hind III, D-Sal I

None of these

Solution

Question 7

$$I-D,II-A,III-B,IV-C$$

$$I-D,II-C,III-B,IV-A$$

$$I-D,II-A,III-C,IV-B$$

$$I-A,II-D,III-B,IV-C$$

Solution

Question 8

Using disarmed pathogen

Microinjection

Gene gun

All of these

Solution

Question 9

Microinjection

Gene gun

Gel electrophoresis

Selection of recombinations

Solution

Question 10

A, B

B, C

C, D

A, B, C, D

Solution

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