Biotechnology P...

Batch Fermentation:

If we want more copies of target (desired) DNA then it should be cloned in a vector having ___________________.

How many fragments will be generated in total upon digestion of a closed circular DNA molecule and liner DNA molecule with a same restriction enzyme having six recognition sites on both DNA molecules? 

Identify the correct sequence of steps in the construction of recombinant DNA.
$$(1)$$ use restriction enzymes
$$(2)$$ use DNA ligase
$$(3)$$ remove plasmid from parent bacterium
$$(4)$$ introduce plasmid into new host bacterium.

Sparger in a bioreactor facilities

E.coli cloning vector pBR322 showing restriction sites, ori and antibiotic resistance genes is given below. Select the correct option.

Column I                                            Column II
I. Agarose                                             A. PCR
II. Opines                                              B. Gene gun
III. Biolistic                                           C. Ti plasmid
iv. Thermal cycler                                 D. Sea weeds

Which of the following techniques can be used to introduce foreign DNA into the cell?

Insertional inactivation is related to

The areas of application of PCR include