Biotechnology and its Applications Test

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Biotechnology and its Applications Test - 46

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Weekly Quiz Competition

Question 1
1 / -0

All of the followings are associated with replacement principle of animal biotechnology except _______.

A
Cell culture

B
Computer models

C
Epidemiological studies

D
Improving experimental technique
Question 2
1 / -0

Identify the food grade vector.

A
Plasmid

B
Phasmid

C
Cosmid

D
All of the above

Solution

Plasmids are important tools in biotechnology laboratories where they are used in order to restore impaired function in food-grade vectors.
Question 3
1 / -0

Identify the correct combination of crop-variety and insect pests.

A
Brassica Pusa - A-4 Aphids

B
Brassica Pusa - sem 3 Jassids

C
Flat beam Pusa Gaurav - Fruit borer

D
Okra Pusa sawani shoot - Fruit borer

Solution

Pusa gaurav variety of brassica is resistant to aphids. Pusa Sem 2 and Pusa Sem 3 of falt beans are resistant to jassids, aphids and fruit borer. Pusa Sawani and Pusa A-4 varieties of okra are resistant to shoot and fruit borer. Correct answer is D.
Question 4
1 / -0

Somatic gene therapy causes ___________.

A
Changes in sperm

B
Changes in progeny

C
Changes in body cell

D
Changes in ovum

Solution

Somatic gene therapy causes changes in the body cells. It involves the placement of a human gene into a living person's somatic cells, except the cells that produce the eggs and sperm. The aim of somatic cell therapy is to cure the patient not the patient’s progeny.
So, the correct answer is option C.
Question 5
1 / -0

Some of the common steps involved in the production of humulin are given below. Choose the correct sequence.
I. Synthesis of the gene (DNA) for human insulin artificially.
II. Culturing of recombinant E. coli in bacteria.
III. Purification of humulin.
IV. Insertion of human insulin gene in plasmid DNA.
V. Introduction of recombinant plasmid into E. coli.
VI. Extraction of recombinant gene product from E. coli.

A
II, I, IV, Ill, V, VI

B
I, III, V, VI, II, IV

C
I, IV, V, II ,VI, III

D
I, V, IV, II ,VI, III

Solution

Humulin, a synthetic version of Insulin, is produced using modern genetic engineering technology. The following steps are involved in the production of humulin:
I. Synthesis of the gene (DNA) for human insulin artificially.
II. Insertion of human insulin gene in plasmid DNA.
III. Introduction of recombinant plasmid into Escherichia coli.
IV. Culturing of recombinant E. coli in bacteria.
V. Extraction of a recombinant gene product from E. coli.
VI. Purification of humulin.
Question 6
1 / -0

One of the possibilities of curing most of the dreaded diseases is

A
Gene replacement therapy

B
Stimulation of endorphins

C
Antisense therapy

D
Both A and C

Solution

Antisense therapy is a form of treatment for genetic disorders or infections. When the genetic arrangement of an appropriate gene is admitted to be innovative of a particular defect, it can be available to synthesize a strand of nucleic acid which will joint with the messenger RNA (mRNA) composed by gene and inactivate it, and thereby adequately pass that particular gene "off". Gene therapy is a technique which uses genes to delight or avoids disease. Instead of using drugs or surgery, gene replacement approach may grant doctors to treat a disorder by inserting a gene into a patient's cells.
So, the correct answer is option D.
Question 7
1 / -0

The correct labelling for the given image is

A
A- Primary antibody, B- Conjugated secondary antibody, C- HRP, D- Substrate

B
A- Conjugated secondary antibody, B- Primary antibody, C- HRP, D- Substrate

C
A- Primary antibody, B- Conjugated secondary antibody, C- Product, D- Substrate

D
A- Primary antibody, B- Conjugated secondary antibody, C- HRP, D- Product
Question 8
1 / -0

In transgenics, the expression of transgene in target tissue is determined by

A
Transgene

B
Promotor

C
Reporter

D
Enhancer

Solution

In a transgenic organism, reporter genes are used to determine whether a particular DNA construct has been successfully introduced into a cell, organ or tissue. Whereas promoter is the region of DNA that initiates the DNA transcription, an enhancer is the sequence of DNA that enhances the transcription when binds to the specific proteic and transgene is the gene that taken from the genome of one organism and introduces into the genome of another organism. Thus, the correct answer is option C.
Question 9
1 / -0

Select the correct option for the given statements X, Y, and Z.
X - A transgenic cow, Rosie produced human protein-enriched milk, which was a nutritionally more balanced product for human babies than natural cow milk.
Y- Milk produced by transgenic cow Rosie contains 2.4 gm protein/L.
Z- In the above-mentioned milk in Y statement, alpha-lactalbumin is present.

A
Statements X, Y, Z are true and statement Z does not give correct explanation of X

B
Statements X, Y, Z are true and statement Z gives correct explanation for X

C
Statements X and Y are correct and statement Z is wrong

D
Statements X and Y are wrong and statement Z is correct

Solution

Alpha-lactalbumin is one of the major proteins in milk (approximately 2.4 g/L). It is considered to be highly nutritious and this is the protein that is not only involved in the synthesis of lactose but is also responsible for milk production. The fact that the milk is nutritionally enriched (statement-X) can be attributed to the increased production of alpha-lactalbumin (statement-Z).
Therefore, the correct option is B.
Question 10
1 / -0

What is the benefit of using a retrovirus as a vector in gene therapy?

A
It is not able to enter cells

B
It eliminates a lot of unnecessary steps

C
It incorporates the foreign gene into the host chromosome

D
Both (b) and (C)

Solution

Retroviruses are a class of enveloped viruses that contain a single-stranded RNA genome. They are able to efficiently integrate permanently into the human genome after entering cells, where they provide the basis for permanent expression of foreign DNA. They offer several benefits like the integration of the gene of interest into chromosomes, stable expression, reducing a lot of unnecessary steps required when we go by gene therapy by other methods and the unique life cycle of the virus causing no immunogenicity.
So, the correct option is 'Both (b) and (C)'.

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Re-Attempt Weekly Quiz Competition

Biotechnology and its Applications Test - 46

Result

Biotechnology and its Applications Test - 46

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SHARING IS CARING

Question 1

Cell culture

Computer models

Epidemiological studies

Improving experimental technique

Question 2

Plasmid

Phasmid

Cosmid

All of the above

Solution

Question 3

Brassica Pusa - A-4 Aphids

Brassica Pusa - sem 3 Jassids

Flat beam Pusa Gaurav - Fruit borer

Okra Pusa sawani shoot - Fruit borer

Solution

Question 4

Changes in sperm

Changes in progeny

Changes in body cell

Changes in ovum

Solution

Question 5

II, I, IV, Ill, V, VI

I, III, V, VI, II, IV

I, IV, V, II ,VI, III

I, V, IV, II ,VI, III

Solution

Question 6

Gene replacement therapy

Stimulation of endorphins

Antisense therapy

Both A and C

Solution

Question 7

A- Primary antibody, B- Conjugated secondary antibody, C- HRP, D- Substrate

A- Conjugated secondary antibody, B- Primary antibody, C- HRP, D- Substrate

A- Primary antibody, B- Conjugated secondary antibody, C- Product, D- Substrate

A- Primary antibody, B- Conjugated secondary antibody, C- HRP, D- Product

Question 8

Transgene

Promotor

Reporter

Enhancer

Solution

Question 9

Statements X, Y, Z are true and statement Z does not give correct explanation of X

Statements X, Y, Z are true and statement Z gives correct explanation for X

Statements X and Y are correct and statement Z is wrong

Statements X and Y are wrong and statement Z is correct

Solution

Question 10

It is not able to enter cells

It eliminates a lot of unnecessary steps

It incorporates the foreign gene into the host chromosome

Both (b) and (C)

Solution

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