Biotechnology Test

Question 1
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Primers are______.

A
Nucleases

B
Fragments of RNA

C
Palindromic sequences

D
Chemically synthesized oligonucleotides

Question 2
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The separation and purification of recombinant protein product is called as______.

A
Extraction

B
Hybridisation

C
Tissue culture

D
Downstream processing

Question 3
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The smallest bands in the agarose gel will be towards

A
Wells

B
Middle of the gel

C
Cathode

D
Anode

Question 4
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Which of the following is not a cloning vector?

A
YAC

B
pBR322

C
Probe

D
BAC

Question 5
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Which of the following contributed in popularising the PCR (polymerase chain reactions) technique?

A
Easy availability of DNA template

B
Availability of synthetic primers

C
Availability of 'Thermostable 'DNA polymerase

D
Availability of cheap deoxyribonucleotides

Question 6
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Given below are two statements: one is labelled as Assertion (A) and the other is labelled as Reason (R).

Assertion (A) : When a particular restriction enzyme cuts strand of DNA, overhanging stretches or sticky ends are formed.

Reason (R) : Some restriction enzymes cut the strand of DNA a little away from the centre of palindromic site.

In the light of the above statements, choose the correct answer from the options given below :

A
(A) is not correct but (R) is correct

B
Both (A) and (R) are correct and (R) is the correct explanation of (A)

C
Both (A) and (R) are correct but (R) is not the correct explanation of (A)

D
(A) is correct but (R) is not correct

Question 7
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Separation of DNA fragments is done by a technique known as

A
Gel electrophoresis

B
Polymerase Chain Reaction

C
Recombinant technology

D
Southern blotting

Question 8
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The enzyme (a) is needed for isolating genetic material from plant cells and enzyme (b) for isolating genetic material from fungus. Choose the correct pair of options from the following :

A
(a) Cellulase (b) Lipase

B
(a) Cellulase (b) Protease

C
(a) Cellulase (b) Chitinase

D
(a) Chitinase (b) Lipase

Question 9
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Given below are two statements : one is labelled as Assertion (A) and the other is labelled as Reason (R).

Assertion (A) : Polymerase chain reaction is used in DNA amplification.

Reason (R) : The ampicillin resistant gene is used as a selectable marker to check transformation.

In the light of the above statements, choose the correct answer from the options given below :

A
Both (A) and (R) are correct and (R) is the correct explanation of (A)

B
Both (A) and (R) are correct but (R) is not the correct explanation of (A)

C
(A) is correct but (R) is not correct

D
(A) is not correct but (R) is correct

Question 10
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Which of the following is correct sequence of process of recombinant DNA technology-
i. Isolation of DNA
ii. Isolation of desired DNA fragment.
iii. Fragmentation of DNA by restriction endonuclease.
iv. Transferring of into host
v. Ligation of DNA fragment into vector
vi. Culturing in host cell to get desired product.

A
Step v, vi, iv, iii, ii and i

B
Step i, ii, iii, iv, v and vi

C
Step i, iii, ii, v, iv and vi

D
Step i, iv, iii, ii, v and vi

Question 11
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Given below are two statements:

Statement I : Restriction endonucleases recognise specific sequence to cut DNA known as palindromic nucleotide sequence.

Statement II : Restriction endonucleases cut the DNA strand a little away from the centre of the palindromic site.

In the light of the above statements, choose the most appropriate answer from the options given below :

A
Both Statement I and Statement II are correct

B
Both Statement I and Statement II are incorrect

C
Statement I is correct but Statement II is incorrect

D
Statement I is incorrect but Statement II is correct

Question 12
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The sequence of the 5 ’and 3 ’primers for the following DNA sequence will be:
5 ’AATGCGGCAATCGAGTC3 ’
3 ’TTACGCCGTTAGCTCCAG5 ’

A
5 ’TTACG 3 ’, 5 ’CTGAG 3 ’

B
5 ’AATGC 3 ’, 5 ’TTACG 3 ’

C
5 ’AATGC 3 ’, 5 ’GACTC 3 ’

D
5 ’AGTC 3 ’, 5 ’TCAG 3 ’

Question 13
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Which of the following is not a desirable feature of a cloning vector?

A
Presence of origin of replication

B
Presence of a marker gene

C
Presence of single restriction enzyme site

D
Presence of two or more recognition sites

Question 14
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The agarose gel with high % of agar will lead to accumulation of large sized DNA fragment near the wells as:

A
Absence of pores

B
Pore size is small

C
Voltage fluctuation with high % of agar

D
Pore size is big

Question 15
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During the purification process for recombinant DNA technology, addition of chilled ethanol precipitates out :

A
Polysaccharides

B
RNA

C
DNA

D
Histones

Submit Test

Biotechnology T...

Question 1

Nucleases

Fragments of RNA

Palindromic sequences

Chemically synthesized oligonucleotides

Question 2

Extraction

Hybridisation

Tissue culture

Downstream processing

Question 3

Wells

Middle of the gel

Cathode

Anode

Question 4

YAC

pBR322

Probe

BAC

Question 5

Easy availability of DNA template

Availability of synthetic primers

Availability of 'Thermostable 'DNA polymerase

Availability of cheap deoxyribonucleotides

Question 6

(A) is not correct but (R) is correct

Both (A) and (R) are correct and (R) is the correct explanation of (A)

Both (A) and (R) are correct but (R) is not the correct explanation of (A)

(A) is correct but (R) is not correct

Question 7

Gel electrophoresis

Polymerase Chain Reaction

Recombinant technology

Southern blotting

Question 8

(a) Cellulase (b) Lipase

(a) Cellulase (b) Protease

(a) Cellulase (b) Chitinase

(a) Chitinase (b) Lipase

Question 9

Both (A) and (R) are correct and (R) is the correct explanation of (A)

Both (A) and (R) are correct but (R) is not the correct explanation of (A)

(A) is correct but (R) is not correct

(A) is not correct but (R) is correct

Question 10

Step v, vi, iv, iii, ii and i

Step i, ii, iii, iv, v and vi

Step i, iii, ii, v, iv and vi

Step i, iv, iii, ii, v and vi

Question 11

Both Statement I and Statement II are correct

Both Statement I and Statement II are incorrect

Statement I is correct but Statement II is incorrect

Statement I is incorrect but Statement II is correct

Question 12

5 ’TTACG 3 ’, 5 ’CTGAG 3 ’

5 ’AATGC 3 ’, 5 ’TTACG 3 ’

5 ’AATGC 3 ’, 5 ’GACTC 3 ’

5 ’AGTC 3 ’, 5 ’TCAG 3 ’

Question 13

Presence of origin of replication

Presence of a marker gene

Presence of single restriction enzyme site

Presence of two or more recognition sites

Question 14

Absence of pores

Pore size is small

Voltage fluctuation with high % of agar

Pore size is big

Question 15

Polysaccharides

RNA

DNA

Histones

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