Each haemoglobin molecule can carry a maximum of four molecules of O2. Binding of oxygen with haemoglobin is primarily related to partial pressure of O2. Partial pressure of CO2, hydrogen ion concentration and temperature are the other factors which can interfere with this binding. A sigmoid curve is obtained when percentage saturation of haemoglobin with O2 is plotted against the pO2. This curve is called the Oxygen dissociation curve and is highly useful in studying the effect of factors like pCO2, H+ concentration, etc., on binding of O2 with haemoglobin. In the alveoli, where there is high pO2, low pCO2, lesser H+ concentration and lower temperature, the factors are all favourable for the formation of oxyhaemoglobin, whereas in the tissues, where low pO2, high pCO2, high H+ concentration and higher temperature exist, the conditions are favourable for dissociation of oxygen from the oxyhaemoglobin.
A shift of the curve to the right indicates decreased affinity of the haemoglobin for oxygen and hence an increased tendency to give up oxygen to the tissues. A shift to the left indicates increased affinity and so an increased tendency for haemoglobin to take up and retain oxygen.
The effect of this rightward shift of the curve increases the partial pressure of oxygen in the tissues when it is most needed, such as during exercise, or hemorrhagic shock. In contrast, the curve is shifted to the left by the opposite of these conditions.