Given: Focal length of the objective lens, \(f_{0}=1.25 \mathrm{~cm}\)
The focal length of the eyepiece, \(f_{e}=5 \mathrm{~cm}\)
Least distance of distinct vision, \(d=25 \mathrm{~cm}\)
Angular magnification of the compound microscope \(=30 \mathrm{X}\)
Total magnifying power of the compound microscope, \(m=30\)
The angular magnification of the eyepiece is given by the relation:
\(m_{e}=\left(1+\frac{d}{f_{e}}\right)\)
\(=\left(1+\frac{25}{5}\right)\)
\(=6\)
The angular magnification of the objective lens \(\left(m_{0}\right)\) is related to \(m_{e}\) as:
\(m_{0} m_{e}=m\)
\(m_{o}=\frac{m}{m_{e}}\)
\(=\frac{30}{6}\)
\(=5\)
We also have the relation:
\(m_{o}=\frac{\text { Image distance for the objective lens }\left(v_{o}\right)}{\text { Object distance for the objective lens }\left(-u_{o}\right)}\)
\(5=\frac{v_{0}}{-u_{0}}\)
Therefore, \(v_{0}=-5 u_{0} \ldots \ldots . . .(1)\)
Applying the lens formula for the objective lens:
\(\frac{1}{f_{0}}=\frac{1}{v_{0}}-\frac{1}{u_{o}}\)
\(\frac{1}{1.25}= \frac{1}{-5u_{0}}-\frac{1}{u_{o}}\quad\because{[v_{0}=-5 \mathrm{u}_{0}] from}(1)\)
\(\frac{1}{1.25}=\frac{-6}{5 u_{0}}\)
Therefore, \(\mathrm{u}_{0}=\frac{-6}{5} \times 1.25\)
\(\mathrm{u}_{0}=-1.5 \mathrm{~cm} \)
\(\text { And } v_{0}=-5 \mathrm{u}_{0} \)
\(=-5 \times(-1.5) \)
\(=7.5 \mathrm{~cm}\)
The object should be placed \(1.5 \mathrm{~cm}\) away from the objective lens to obtain the desired magnification. Applying the lens formula for the eyepiece:
\(\frac{1}{f_{e}}=\frac{1}{v_{e}}-\frac{1}{u_{e}}\)
Where,
\(v_{e}=\) Image distance for the eyepiece \(=-d=-25 \mathrm{~cm}\)
\(\mathrm{u}_{\mathrm{e}}=\) Object distance for the eyepiece
\(\frac{1}{u_{e}}=\frac{1}{v_{e}}-\frac{1}{f_{c}} \)
\(\frac{1}{u_{e}}=\frac{-1}{25}-\frac{1}{5}\)
\(\frac{1}{u_{e}}=\frac{-6}{25}\)
Therefore, \(\mathrm{u}_{\mathrm{e}}=-4.17 \mathrm{~cm}\)
Separation between the objective lens and the eyepiece \(=\left|u_{e}\right|+\left|v_{o}\right|\)
\(=4.17+7.5 \)
\(=11.67 \mathrm{~cm}\)
Therefore, \(11.67 \mathrm{~cm}\) is the separation between the objective lens and the eyepiece.