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Biotechnology Principles and Processes Test - 16

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Biotechnology Principles and Processes Test - 16
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  • Question 1
    1 / -0
    The .......... enzyme is used to cut DNA at specific point.
    Solution

    Restriction endonucleases are enzymes which scan the DNA molecule for a particular nucleotide sequence. These are called recognition sequences. Once the endonuclease finds this sequence it halts and cuts the strand. Thus the correct answer is option C.
  • Question 2
    1 / -0
    DNA taq polymerase enzyme is isolated from which bacteria?
    Solution
    DNA polymerase enzyme was isolated from a thermophilc bacteria called Thermus aquaticus. This is an enzyme used frequently to make multiple copies of segment of DNA by a technique called polymerase chain reaction.
  • Question 3
    1 / -0
    Polymerase chain reaction is useful in
    Solution
    Polymerase chain reaction (PCR) is a method widely used in molecular biology to make many copies of a specific DNA segment. Using PCR, a single copy (or more) of a DNA sequence is exponentially amplified to generate thousands to millions of more copies of that particular DNA segment.
    So, the correct answer is 'Amplification of specific DNA segment.'
  • Question 4
    1 / -0
    PCR technique was invented by
    Solution

  • Question 5
    1 / -0
    Reaction of 1989 was
    Solution
    In 1985 Norm Arnheim, also a member of the development team concluded his sabbatical at Cetus and assumed an academic position at USC. He began to investigate the use of PCR to amplify samples containing just a single copy of the target sequence. By 1989 his lab developed multiplex-PCR on single sperm to directly analyze the products of meiotic recombination.
    So, the correct answer is 'Polymerase chain reaction.'
  • Question 6
    1 / -0
    Recombinant DNA technology developed during
    Solution
    The correct option is A
    rDNA technology is the way in which genetic material from one organism is artificially introduced into the genome of another organism and then replicated and expressed by other organism. It was advanced by the collaboration of stanley cohen and Herbert Boyer in $$1972$$.
  • Question 7
    1 / -0
    During PCR technique the pairing of primers to ssDNA segment is called
    Solution
    PCR - technique amplify the DNA sample in three steps namely denaturation of target DNA, annealing and polymerization. Denaturation refers to the thermal cycle that separates the DNA strands and forms the ssDNA. Annealing refers to the pairing of primers to the ssDNA and polymerisation is the extension of primer into complete DNA strand complementary to the template strand. 
    So, the correct answer is 'Annealing'
  • Question 8
    1 / -0
    Bacteria protect themselves from viruses by fragmenting viral DNA upon entry with _____________.
    Solution
    Restriction enzyme, also called restriction endonuclease, a protein produced by bacteria that cleaves DNA at specific sites along the molecule. In the bacterial cell, restriction enzymes cleave foreign DNA, thus eliminating infecting organisms. A bacterium uses a restriction enzyme to defend against bacterial viruses called bacteriophages, or phages. When a phage infects a bacterium, it inserts its DNA into the bacterial cell so that it might be replicated. The restriction enzyme prevents replication of the phage DNA by cutting it into many pieces. Restriction enzymes were named for their ability to restrict, or limit, the number of strains of bacteriophage that can infect a bacterium.

    So, the correct answer is 'Endonucleases'
  • Question 9
    1 / -0
    In which field, application of biotechnology occurs?
    Solution
    Application of biotechnology occurs in agriculture, biomedicine and environmental field.
  • Question 10
    1 / -0
    "Gray biotechnology" is?
    Solution
    White or gray biotechnolgy involves industrial processes such as the development of new chemicals or production of new fuel for vehicles.
    Correct  answer-A
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