Biotechnology Principles and Processes Test

Result

Biotechnology Principles and Processes Test - 32

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Weekly Quiz Competition

Question 1
1 / -0

Endonucleases break DNA at specific sites called

A
Palindromic sequences

B
Restriction sites

C
Conserved sites

D
Both A and B

Solution

Restriction enzyme, also called restriction endonuclease, a protein produced by bacteria that cleaves DNA at palindromic sequences and restriction sites along the molecule. In bacterial enzymes cleave foreign DNA, thus eliminating infecting organisms.
So, option D is the correct option.
Question 2
1 / -0

Enzyme that cuts DNA at specific sites is

A
DNA ligase

B
Restriction endonuclease

C
DNA polymerase

D
Reverse transcriptase

Solution

A restriction enzyme, restriction endonuclease is an enzyme that cleaves DNA into fragments at or near specific recognition sites within molecules known as restriction sites. Restriction enzymes are one class of the broader endonuclease group of enzymes.
So, option B is the correct option.
Question 3
1 / -0

Arber, Smith and Nathans are famous for discovery of

A
Gene therapy

B
Restriction enzyme

C
Humulin

D
Second generation vaccines

Solution
Question 4
1 / -0

Structure involved in genetic engineering is

A
Plastid

B
Restriction endonuclease

C
DNA polymerase I

D
Prochromosome

Solution

The structure involved in genetic engineering is Restriction endonuclease. So the correct option is B.
Question 5
1 / -0

Restriction endonuclease is employed for cutting

A
A single stranded DNA

B
Double stranded DNA

C
RNA fragment

D
mRNA

Solution

So the correct option is B.
Question 6
1 / -0

Which enzyme is useful in genetic engineering?

A
DNA-ase

B
Amylase

C
Lipase

D
Restriction endonuclease

Solution

A restriction enzyme, restriction endonuclease is an enzyme that cleaves DNA into fragments at or near specific recognition sites within molecules known as restriction sites. In the bacterial cell, restriction enzymes cleave foreign DNA, thus eliminating infecting organisms.
So, option D is the correct option.
Question 7
1 / -0

Enzyme required for polymerase chain reaction (PCR) is

A
RNA polymerase

B
Ribonuclease

C
Taq polymerase

D
Endonuclease

Solution

Like DNA replication in an organism, PCR requires a DNA polymerase enzyme that makes new strands of DNA, using existing strands as templates. The DNA polymerase typically used in PCR is called Taq polymerase, after the heat - tolerant bacterium from which it was isolated Thermus aquaticus.
So, option C is the correct option.
Question 8
1 / -0

Restriction endonucleases are

A
Used in genetic engineering for uniting two DNA molecules

B
Used for in vitro DNA synthesis

C
Present in mammalian cells for degeneration of DNA of dead cells

D
Synthesised by bacteria for their defence
Solution
Correct Option: D
Explanation:
Endonucleases identify certain sequences in incoming DNA and digest it into pieces.
They either cut the DNA at specified locations or more randomly.
As a result, bacteria produce restriction endonucleases as part of their defence mechanism.
Hence, Restriction Endonuclease are synthesised by bacteria for their defence.
Question 9
1 / -0

Chemical knives/molecular scissors/genetic scalpels of DNA are

A
Restriction endonucleases

B
Polymerases

C
Ligases

D
Transcriptases

Solution

A restriction enzyme, restriction endonuclease is an enzyme that cleaves DNA into fragments at or near specific recognition sites within molecules known as restriction sites. In the bacterial cell, restriction enzymes cleave foreign DNA, thus eliminating infecting organisms. Restriction endonuclease is also known as molecular scissors as it cuts the DNA at the restriction sites.
So, option A is the correct option.
Question 10
1 / -0

The technique of insertion of a desired gene into DNA of plasmid vector is

A
Gene splicing

B
Gene dressing

C
Gene cloning

D
Gene drafting
Solution
Cloning is a process in which the DNA segments are joined together and produced in multiple copies in the host cell.
Generally, one end of each of the two-segment is connected to achieve the new genetic combination.
The plasmids are used as vectors to transfer this rDNA into the cell where multiple copies of the DNA fragments can be produced.
The method of genetic recombination is often used in the process of cloning

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Re-Attempt Weekly Quiz Competition

Biotechnology Principles and Processes Test - 32

Result

Biotechnology Principles and Processes Test - 32

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SHARING IS CARING

Question 1

Palindromic sequences

Restriction sites

Conserved sites

Both A and B

Solution

Question 2

DNA ligase

Restriction endonuclease

DNA polymerase

Reverse transcriptase

Solution

Question 3

Gene therapy

Restriction enzyme

Humulin

Second generation vaccines

Solution

Question 4

Plastid

Restriction endonuclease

DNA polymerase I

Prochromosome

Solution

Question 5

A single stranded DNA

Double stranded DNA

RNA fragment

mRNA

Solution

Question 6

DNA-ase

Amylase

Lipase

Restriction endonuclease

Solution

Question 7

RNA polymerase

Ribonuclease

Taq polymerase

Endonuclease

Solution

Question 8

Used in genetic engineering for uniting two DNA molecules

Used for in vitro DNA synthesis

Present in mammalian cells for degeneration of DNA of dead cells

Synthesised by bacteria for their defence

Solution

Question 9

Restriction endonucleases

Polymerases

Ligases

Transcriptases

Solution

Question 10

Gene splicing

Gene dressing

Gene cloning

Gene drafting

Solution

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