Biotechnology Principles and Processes Test

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Biotechnology Principles and Processes Test - 33

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Weekly Quiz Competition

Question 1
1 / -0

Genetic engineering has been made possible due to

A
Observation of DNA under electron microscope

B
We can break DNA at specific points by DNA-ases

C
Availability of restriction endonucleases in purified form

D
Knowledge of transduction

Solution

Genetic engineering has been made possible due to availability of restriction endonucleases in purified form. Restriction endonuclease is an enzyme that cleaves DNA into fragments at or near specific recognition sites within molecules known as restriction sites. In bacterial cell, restriction enzymes cleave foreign DNA, thus eliminating infecting organisms.
So, option C is the correct option.
Question 2
1 / -0

Nucleic acid is fragmented by enzyme

A
Ligases

B
Proteases

C
Nucleases

D
Polymerases

Solution

Nucleases are the enzyme that breakdown nucleic acid. Polymerase enzyme catalyze the production of polymeric form of nucleic acid.
So, option C is the correct option.
Question 3
1 / -0

Construction of recombinant DNA involves ________.

A
Cleaving and rejoining of DNA segments with endonuclease

B
Cleaving DNA segments with endonuclease and rejoining with ligase

C
Cleaving and rejoining DNA segments with ligase

D
Cleaving DNA segments with ligase and rejoining with endonuclease

Solution

Recombinant DNA is made by combining DNA from two or more sources. DNA fragments are cut out of a specific position in the chromosome using restriction enzymes (also called restriction endonuclease) and then inserted into other chromosomes or DNA molecules using enzyme ligase.
So, option B is the correct option.
Question 4
1 / -0

The enzymes which are commonly used in genetic engineering are

A
Restriction endonuclease and polymerase

B
Endonuclease and ligase

C
Restriction endonuclease and ligase

D
Ligase and polymerase

Solution

Recombinant DNA is made by combining DNA from two or sources. DNA fragments are cut out of their specific position in the chromosome using restriction enzymes (also called restriction endonuclease) and then inserted into other DNA molecules using enzymes DNA ligase.
So, option C is the correct option.
Question 5
1 / -0

Endonuclease is employed in

A
Transcription

B
Translation

C
Genetic engineering

D
DNA replication
Question 6
1 / -0

Thermal cycle is used in

A
Radioactivation

B
Chemical reaction

C
Polymerase chain reaction

D
Enzyme catalysed reactions

Solution

The thermal cycler is a laboratory apparatus most commonly used to amplify segments of DNA via the polymerase chain reaction (PCR). The cycler then raises and lowers the temperature of the block in discrete, pre-programmed steps.
So, option C is the correct option.
Question 7
1 / -0

Which one is a wrong statement?

A
Transposons were visualised by Barbara Mc-Clintock

B
DNA ligases are used to cleave DNA molecules

C
Udder cell was used to produce cloned sheep

D
In pedigree analysis, a person immediately affected is called propositus Ian Wilmut produced cloned sheep Dolly
Solution
Correct Option: B
Explanation:
Purified DNA ligase is used in gene cloning to connect DNA molecules to create recombinant DNA.
DNA ligase is an enzyme that catalyses the creation of a phosphodiester link, which aids in the joining of DNA strands.
Ligases are capable of joining any DNA segment with 'blunt' ends.
Hence, the wrong statement is DNA ligases are used to join the DNA strands.
Question 8
1 / -0

Electroporation is

A
Making transient pores in cell membranes to introduce gene constructs

B
Fast passage of nutrients through phloem sieve pores by electric stimulation

C
Opening of stomata by artificial light during night

D
Purification of saline water with the help of membrane system

Solution

Electroporation is now used to transfer the foreign DNA into the fragile cells. The electric pulses induce the formation of large pores in the cell membrane.
These pores give a passage through which the foreign DNA can enter into the protolasts and thus, increase the transformation frequency.
So, option A is the correct option.
Question 9
1 / -0

The most extensively used bacteria in genetic engineering is ____________.

A
Bacillus

B
Clostridium

C
Escherichia

D
Salmonella

Solution

Artificial gene manipulation is known as genetic engineering. The bacterium Escherichia coli is widely used in genetic engineering experiments as:
(1) Human insulin chains are synthesized within the E.coli.
(2) Hormone somatostatin is also produced within the bacterium E. coli.
So, option C is the correct option.
Question 10
1 / -0

Restriction endonucleases are useful in

A
Breaking DNA at specific sites

B
Creating sticky ends

C
Both A and B

D
Crossing over

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Re-Attempt Weekly Quiz Competition

Biotechnology Principles and Processes Test - 33

Result

Biotechnology Principles and Processes Test - 33

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SHARING IS CARING

Question 1

Observation of DNA under electron microscope

We can break DNA at specific points by DNA-ases

Availability of restriction endonucleases in purified form

Knowledge of transduction

Solution

Question 2

Ligases

Proteases

Nucleases

Polymerases

Solution

Question 3

Cleaving and rejoining of DNA segments with endonuclease

Cleaving DNA segments with endonuclease and rejoining with ligase

Cleaving and rejoining DNA segments with ligase

Cleaving DNA segments with ligase and rejoining with endonuclease

Solution

Question 4

Restriction endonuclease and polymerase

Endonuclease and ligase

Restriction endonuclease and ligase

Ligase and polymerase

Solution

Question 5

Transcription

Translation

Genetic engineering

DNA replication

Question 6

Radioactivation

Chemical reaction

Polymerase chain reaction

Enzyme catalysed reactions

Solution

Question 7

Transposons were visualised by Barbara Mc-Clintock

DNA ligases are used to cleave DNA molecules

Udder cell was used to produce cloned sheep

In pedigree analysis, a person immediately affected is called propositus Ian Wilmut produced cloned sheep Dolly

Solution

Question 8

Making transient pores in cell membranes to introduce gene constructs

Fast passage of nutrients through phloem sieve pores by electric stimulation

Opening of stomata by artificial light during night

Purification of saline water with the help of membrane system

Solution

Question 9

Bacillus

Clostridium

Escherichia

Salmonella

Solution

Question 10

Breaking DNA at specific sites

Creating sticky ends

Both A and B

Crossing over

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