Biotechnology Principles and Processes Test

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Biotechnology Principles and Processes Test - 35

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Weekly Quiz Competition

Question 1
1 / -0

Source of Taq polymerase used in PCR is a

A
Thermophilic fungus

B
Mesophilic fungus

C
Thermophilic bacterium

D
Halophilic bacterium

Solution

Taq polymerase is a thermostable DNA polymerase I obtained from the thermophilic bacterium Thermus aquaticus.
Question 2
1 / -0

There is a restriction endonuclease called EcoRI. What does 'co' stand for?

A
Coenzyme

B
Coli

C
Colon

D
Coelom
Question 3
1 / -0

Which of the following products are obtained with the help of biotechnology?

A
Transgenic crop

B
Biofertilizer

C
Humulin

D
All the above

Solution

Genetic engineering, also called genetic modification, is the direct manipulation of an organisms genome using biotechnology. It is a set of technologies used to change the genetic makeup of the cells, including the transfer of genes within and across species boundaries to produce novel organisms like transgenic crop, biofertilizer, humulin.
So, option D is the correct option.
Question 4
1 / -0

Restriction enzymes are also called

A
Molecular markers

B
Vectors

C
Carriers

D
Molecular scissors

Solution

Restriction enzymes are also called molecular scissors. These are the enzymes that cleave the DNA at specific sites called restriction sites. They make a single cut on both the DNA strands.
Thus the correct answer is option D.
Question 5
1 / -0

Polymerase chain reaction employs

A
Primers and DNA ligase

B
DNA ligase only

C
DNA polymerase only

D
Primers and DNA polymerase
Solution
- The polymerase chain reaction is defined as DNA replication in vitro. It employs a DNA template, two nucleotide primers, and a DNA polymerase enzyme. DNA template to be amplified.
- The primers which are oligonucleotides, that hybridize to the target DNA region, one to each strand of the double helix are required. These two primers are oriented with each other facing ends to allow the synthesis of DNA.
- The DNA polymerase required which is stable at high temperature (Taq polymerase, Vent polymerase) for the synthesis of DNA.
- So, the correct answer is option D.
Question 6
1 / -0

DNA polymerase or taq enzyme used in PCR is isolated from

A
Thermus aquaticus

B
E. coli

C
Salmonella typhimurium

D
None of these

Solution

Taq polymerase is a thermostable DNA polymerase I obtained from the thermophilic bacterium Thermus aquaticus.
Question 7
1 / -0

In the diagram of pBR 322, which identifies components correctly:

A
rop-reduced osmotic pressure

B
Hind III, EcoRI selectable markers

C
Am$$p^R$$, te$$t^R$$ antibiotic resistance genes

D
ori original restriction enzyme

Solution

Given diagram is a plasmid which has following characteristics:-
Restriction sites: BamH I, Hind III, Sal I, Pvu I, Pvu II, Pst I, EcoR I, Cla I
Selectable marker: antibiotic resistance genes for ampicillin (ampR) and tetracycline
(tetR).
ORI: the origin of replication
ROP: It codes for proteins, which are involved in the process of replication of plasmid.
So the correct answer is option C.
Question 8
1 / -0

Melting of DNA at 70$$^o$$ C is due to breakdown of

A
Phosphodiester bonds

B
Hydrogen bonds

C
Glycosidic bonds

D
Disulphide bonds

Solution

Dissociation of the double-stranded DNA helix into single coils is referred to as DNA melting. It can be accomplished by simply heating double-stranded DNA. The temperature at which the DNA strands dissociates into single coils depends on the number of hydrogen bonds holding the complementary strands. When DNA is heated at 70°C the hydrogen bonds are broke and caused DNA melting.
So the correct answer is option B.
Question 9
1 / -0

Microparticles for coating with DNA to be bombarded with gene gun are made of

A
Silver or platinum

B
Platinum or zinc

C
Silicon or platinum

D
Gold or tungsten

Solution

For gene transfer into the host cell without using vector microparticles made of tungsten and gold coated with foreign DNA are bombarded into target cells at a very high velocity. This method is called biolistics or gene gun which is suitable for plants.
So the correct answer is option D.
Question 10
1 / -0

Biolistic gun is suitable for

A
Transformation of plant cells

B
Disarming pathogen vectors

C
DNA finger printing

D
Constructing recombinant DNA

Solution

A gene gun is used for delivery of exogenous DNA to cells. This method is known as 'biolistics'. Gene guns can be used effectively on most cells but are mainly used on plant cells.
So the correct answer is option A.

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Re-Attempt Weekly Quiz Competition

Biotechnology Principles and Processes Test - 35

Result

Biotechnology Principles and Processes Test - 35

Score

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SHARING IS CARING

Question 1

Thermophilic fungus

Mesophilic fungus

Thermophilic bacterium

Halophilic bacterium

Solution

Question 2

Coenzyme

Coli

Colon

Coelom

Question 3

Transgenic crop

Biofertilizer

Humulin

All the above

Solution

Question 4

Molecular markers

Vectors

Carriers

Molecular scissors

Solution

Question 5

Primers and DNA ligase

DNA ligase only

DNA polymerase only

Primers and DNA polymerase

Solution

Question 6

Thermus aquaticus

E. coli

Salmonella typhimurium

None of these

Solution

Question 7

rop-reduced osmotic pressure

Hind III, EcoRI selectable markers

Am$$p^R$$, te$$t^R$$ antibiotic resistance genes

ori original restriction enzyme

Solution

Question 8

Phosphodiester bonds

Hydrogen bonds

Glycosidic bonds

Disulphide bonds

Solution

Question 9

Silver or platinum

Platinum or zinc

Silicon or platinum

Gold or tungsten

Solution

Question 10

Transformation of plant cells

Disarming pathogen vectors

DNA finger printing

Constructing recombinant DNA

Solution

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