Biotechnology Principles and Processes Test

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Biotechnology Principles and Processes Test - 40

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Weekly Quiz Competition

Question 1
1 / -0

Restriction enzymes cut DNA at specific sites known as ______________.

A
Telomeric sequences

B
Palindromic sequences

C
Terminator sequences

D
Attenuator sequences

Solution

B. Palindromic sequences
Solution : Restriction enzymes can cut at specific sites which resemble palindrome in nature having palindromic DNA sequences ( GAATTC-CTTAAG)
So the correct answer is "Palindromic sequence"
Question 2
1 / -0

Which of the following does not produce sticky ends?

A
Sma I

B
EcoRI

C
SalI

D
BamHI

Solution

A. SmaI
Solution : SmaI is a restriction enzyme that creates blunt ends during cutting DNA or cut ends end in a base pair whereas sticky ends has base overhangs protruding.
So the correct answer is " SmaI"
Question 3
1 / -0

Most suitable method of introducing alien DNA into a plant cell is

A
Biolistics

B
Micro-injection

C
Lipofection

D
Heat shock method

Solution

So the correct option is A.
Question 4
1 / -0

Taq polymerase used in polymerase chain reaction is active at temperature of

A
25$$^o$$ - 30$$^o$$ C

B
35$$^o$$ - 50$$^o$$ C

C
50$$^o$$ - 70$$^o$$ C

D
72$$^o$$ - 90$$^o$$C

Solution

So the correct option is D.
Question 5
1 / -0

Which of the following characteristics make the end nuclease a restriction enzyme?
a. Restriction infection of bacteria by certain viruses(bacteriophages) by degrading the viral DNA.
b. Ability to cut only the double-stranded DNA
c. Ability to cut DNA into specific fragments containing identifiable genes, at any position.
Select the correct answer using the codes given.

A
$$1$$ and $$2$$

B
$$1$$ and $$3$$

C
$$2$$ and $$3$$

D
$$1, 2$$ and $$3$$

Solution

C. 2 and 3
Solution : Restriction enzymes can cut double stranded DNA and can also cut DNA in identifying or specific positions. All these characters are present in end nuclease that makes it apt for restriction enzyme in recombinant DNA technology.
So the correct answer is " 2 and 3".
Question 6
1 / -0

Polymerase chain reaction technique is used for.

A
DNA amplification

B
Screening gene libraries

C
Synthesis of polymerase

D
DNA sequencing

Solution

Polymerase chain reaction ( PCR), a technique used to make numerous copies of a specific segment of DNA quickly and accurately.
So the correct answer is option A.
Question 7
1 / -0

DNA is amplified by __________________.

A
PCR technique

B
Southern Blotting technique

C
Northern Blotting technique

D
Nucleic acid hybridization technique

Solution

A. PCR technique
Solution : PCR or polymerase chain reaction is technique where a small fragment of DNA is used to create millions of copies in a very short time with the help of primers.
So the correct answer is " PCR technique"
Question 8
1 / -0

Who of the following is associated with the discovery of 'Restriction endonuclease'.

A
Hamilton Smith

B
H. G. Khorana

C
Marshall Nirenberg

D
S. B. Weiss

Solution

A. Hamilton smith
Solution : Hamilton smith along with Werner Arber and Daniel Nathan discovered restriction endonuclease in 1960s.
So the correct answer is " Hamilton Smith"
Question 9
1 / -0

Internal phosphodiester bond in a nucleic acid molecule is hydrolysed by _______________.

A
Exonuclease

B
Endonuclease

C
DNA polymerase

D
Deoxyribonucleic acid
Solution
- In DNA and RNA, the phosphodiester bond connects a 3'-carbon to a 5'-carbon which is hydrolyzed by the endonuclease restriction enzyme.
- Endonuclease makes cuts within the DNA strands while exonuclease removes nucleotides from the end part of the DNA.
- DNA polymerase enzymes synthesize DNA molecules from deoxyribonucleotides.
Hence, the correct answer is option B, i.e., Endonuclease.
Question 10
1 / -0

Insect-resistant transgenic cotton has been produced by inserting a piece of DNA from?

A
An insect

B
A bacterium

C
A wild relative of cotton

D
A virus

Solution

Scientists inserted a toxin gene from the bacterium called Bt (which is the nickname for Bacillus thuringiensis) into cotton plants to create a caterpillar-resistant variety. The gene is DNA that carries the instructions for producing a toxic protein.
So the correct answer is option B.

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Re-Attempt Weekly Quiz Competition

Biotechnology Principles and Processes Test - 40

Result

Biotechnology Principles and Processes Test - 40

Score

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SHARING IS CARING

Question 1

Telomeric sequences

Palindromic sequences

Terminator sequences

Attenuator sequences

Solution

Question 2

Sma I

EcoRI

SalI

BamHI

Solution

Question 3

Biolistics

Micro-injection

Lipofection

Heat shock method

Solution

Question 4

25$$^o$$ - 30$$^o$$ C

35$$^o$$ - 50$$^o$$ C

50$$^o$$ - 70$$^o$$ C

72$$^o$$ - 90$$^o$$C

Solution

Question 5

$$1$$ and $$2$$

$$1$$ and $$3$$

$$2$$ and $$3$$

$$1, 2$$ and $$3$$

Solution

Question 6

DNA amplification

Screening gene libraries

Synthesis of polymerase

DNA sequencing

Solution

Question 7

PCR technique

Southern Blotting technique

Northern Blotting technique

Nucleic acid hybridization technique

Solution

Question 8

Hamilton Smith

H. G. Khorana

Marshall Nirenberg

S. B. Weiss

Solution

Question 9

Exonuclease

Endonuclease

DNA polymerase

Deoxyribonucleic acid

Solution

Question 10

An insect

A bacterium

A wild relative of cotton

A virus

Solution

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