Biotechnology P...

To carry out a polymerase chain reaction (PCR), one must have the catalytic DNA polymerase and

Study the given figure carefully and select the incorrect statements regarding this.(i) It represents a typical agarose gel electrophoresis in which lane 1 contains undigested DNA(ii) Smallest DNA bands are formed at A and largest DNA bands are formed at B(iii) The separated DNA fragments can be visualized after staining in the visible light(iv) The separated DNA bands are cut out from the agarose gel and extracted from the gel piece. This step is known as elution.

Gel electrophoresis is used for

The sticky ends of a fragmented DNA molecule are made up of ________.

The restriction enzyme responsible for the cleavage of following sequence is ______________.

If a plasmid vector is digested with EcoRI at a single site, then

Which of the following statements is not correct regarding EcoRI restriction endonuclease enzyme?

During insertional inactivation, the presence of a chromogenic substrate gives blue coloured colonies if the plasmid in the bacteria does not have an insert. The blue colour is produced by the enzyme