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Biotechnology P...

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  • Question 1
    1 / -0

    Which one of the following is not a correct match?

  • Question 2
    1 / -0

    Enzyme' Taq polymerase' used in PCR, has been isolated from bacterium _________________.

  • Question 3
    1 / -0

    In biolistic method of gene transfer, the microparticles coated with foreign DNA are bombarded into target cells at a very high velocity. These microparticles are made up of

  • Question 4
    1 / -0

    Which of the following is not used to transfer the recombinant DNA into the host?

  • Question 5
    1 / -0

    Given table gives an account of differences between PCR and gene cloning. Which of the, following points shows the incorrect difference?



     





    Parameter





    PCR





    Gene cloning





    1





    Efficient





    More





    Less





    2





    Apparatus Requirement





    DNA





    Restriction enzyme, ligase, vector, bacterial cell





    3





    Manipulation





    In vitro





    In vitro and in vivo





    4





    Cost





    More





    Less





    5





    Automation





    Yes





    No





    6





    Error probability





    Less





    More





    7





    Time for a typical experiment





    2-4 days





    4 hours





    8





    Application





    More





    Less




  • Question 6
    1 / -0

    In a polymerase chain reaction, temperature required for the steps (i) Denaturation,(ii) Annealing, and (iii) Extension are respectively.

  • Question 7
    1 / -0

    Which of the following statements are correct with respect to a bioreactor?
    (i) It can process large volumes of culture(ii) It provides optimum temperature and pH(iii) It is a completely automated tool(iv) It is a compact thermal cycler.

  • Question 8
    1 / -0

    The correct sequence of different steps of polymerase chain reaction is

  • Question 9
    1 / -0

    In addition to Taq polymerase enzyme which other thermostable DNA polymerases have been isolated to be used in polymerase chain Reaction (PCR)?

  • Question 10
    1 / -0

    If a recombinant DNA bearing gene for resistance to antibiotic ampicillin is transferred to E.coli cells, the host cells become transformed into ampicillin-resistant cells. If such bacteria are transferred on agar plates containing ampicillin, only transformants will grow and the non-transformed recipient cells will die. The ampicillin-resistant gene in this case is called as _______________.

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