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Biotechnology Principles and Processes Test - 51

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Biotechnology Principles and Processes Test - 51
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  • Question 1
    1 / -0
    X technique is now routinely used to detect HIV in suspected AIDs patients. It is being used to detect mutations in genes in suspected cancer patients too. It is a powerful technique to identify many other genetic disorders. Identify X-
    Solution
    The development of molecular techniques that access viral load and the development of genotypic resistance have revolutionized the treatment of HIV disease. Commercially available viral load assays use a number of different approaches from reverse transcriptase PCR to amplification of branched chain DNA. New real-time PCR assays are under development, including LightCycler- and TaqMan-based tests. So, the correct answer is option A. ( X=PCR ).
  • Question 2
    1 / -0
    The first plasmid used for recombinant DNA technology is obtained from
  • Question 3
    1 / -0
    Which of the following is not a source of restriction endonuclease?
    Solution
    • Agrobacterium tumefaciens is a pathogen of several dicot plants. It delivers a piece of DNA known as T-DNA' in the Ti plasmid which transforms normal plant cells into tumor cells to produce chemicals against pathogens. 
    • The restriction enzyme Eco Rl, is isolated from Escherichia coli RY13. The first restriction enzyme Hind II was isolated from the bacterium Haemophilus influenzas. 
    • The restriction enzyme Bam HI is isolated from Bacillus amyloli. 
    So, the correct option is option C.
  • Question 4
    1 / -0
    If a pBR322 vector is cut by ECORI enzyme than which of its marker gene shows insertional inactivation-
    Solution
    Correct option: D

    Explanation:
    • If a pBR322 vector is cut by ECORI enzyme, then insertional inactivation will be seen in the restriction sites of Cla I and Hind III, between tetR and ampR markers.
    • Thus, Option D - none of these is correct.

  • Question 5
    1 / -0
    Which of the following can't be achieved using PCR ?
  • Question 6
    1 / -0
    Gene transfer is present in:-
    Solution
    • Biolistics including particle bombardment is a commonly used method for genetic transformation of plants when either cells/tissues or intracellular organelles are impermeable to foreign DNA. The plant cell wall is usually impermeable to foreign DNA. 
    • Utilization of the gene gun method has shown better results against the various barriers that hinder the delivery of foreign DNA inside the organelle/cell/tissue. Gene transfer through a gene gun is part of the biolistic method. During this method DNA or RNA construct adheres to biological inert particles (such as gold or tungsten) to form a DNA/particle complex. 
    • The DNA/particle complex is bombarded on a targeted tissue in a vacuum. This bombardment is achieved by accelerating a powerful shot to the targeted tissue. The high density of the DNA/particle complex increases the bombardment speed toward the targeted tissue, which may result in effective transformation. 
    • Only inert metal particles can be bombarded through a solution containing DNA surrounding the cell. So, Biolistic (gene gun) is a technique in genetic engineering in which tiny metal pellets coated with DNA are propelled into living cells at high velocities.
    So, the correct option is option A.
  • Question 7
    1 / -0
    The process by which restriction endonuclease cut the DNA molecule?
  • Question 8
    1 / -0
    In E.coli, a finished polypeptide has $$162$$ amino acids of which the first amino acid is not methionine compound. How many nucleotides of DNA are required to code this polypeptide?
    Solution
    There is an intimate connection between genes and synthesis of polypeptides or enzymes The relationship between the sequence, of amino acids in a polypeptide and nucleotide sequence of DNA or mRNA is called genetic code. To code a polypeptide of 162 amino acids 486 nucleotides required but here, methionine is absent. Therefore, 489 nucleotides required and methionine may remove from polypeptide later.
    So, the correct option is option C.
  • Question 9
    1 / -0
    Which of the following technique is employed to check the progression of a restriction enzyme digestion?
    Solution
    Answer is option C i.e. "Gel electrophoresis"
    Gel electrophoresis is the technique which can check DNA fragment progression. Restriction enzyme digest DNA into fragments. Each fragment has different size. When gel electrophoresis is performed each fragment with different sizes are separated on the gel.
    While, PCR is used for DNA amplification. Bioreactors are the fermentors used for fermentation process and micro-injection is a method used to transfer genes between animals.
  • Question 10
    1 / -0
    The technique in which a foreign DNA is precipitated on the surface of the tungsten or gold particle and shot into target cells is known as? 
    Solution
    • In microinjection, a glass-made micropipette is used for DNA transfer.
    • The chemical-mediated genetic transformation uses chemical agents.
    • In Electroporation, the target cells are mixed and treated with an electric field to create pores for DNA entry.
    • In the biolistic method or gene gun method, the foreign DNA is precipitated over the surface of metals like; gold or tungsten, and bullets are prepared.
    • These bullets are then fired into the solution of target cells. This foreign DNA gets inserted and helps in the production of desired products.
    So, the correct option is option D. 
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