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Biotechnology P...

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  • Question 1
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    Gene recombinant technology is used for

  • Question 2
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    Restriction enzymes were discovered by microbiologists who were studying the host range of infectious bacteriophage. The term restriction refers to the fact that different strains of bacteria are resistant to bacteriophage infection if they contain the appropriate endonucleases that can destroy infecting DNA. Observe this figure and answer the following questions.

    What are structures labeled A and B respectively?

  • Question 3
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    Polyethylene glycol method is used for

  • Question 4
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    Which one is a true statement regarding DNA polymerase used in PCR?

  • Question 5
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    Restriction enzymes were discovered by microbiologists who were studying the host range of infectious bacteriophage. The term restriction refers to the fact that different strains of bacteria are resistant to bacteriophage infection if they contain the appropriate endonucleases that can destroy infecting DNA. Observe this figure and answer the following questions.

    Can you pick up from figure, how the bacteria protects its own genome from degradation by restriction endonucleases?

  • Question 6
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    Which one of the following palindromic bases sequences in DNA, can be easily cut at about the middle by some particular restriction enzyme?

  • Question 7
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    PEG is used for

  • Question 8
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    Eco RI, Eco RV and Sac I are the types of restriction enzymes. Three types of termini can be generated.

    (1) 5' staggered ends, 
    (2) blunt ends, and 
    (3) 3'staggered ends. Importantly, the 5' termini of each strand in the cleavage product(s) retain the phosphoryl group from the phospho diester bond, the 3' termini are hydroxylated. Here are their actions given. 
    Which of the following is correct answer?

  • Question 9
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    Protection of endogenous cleavage is accomplished by

  • Question 10
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    In recombination DNA technique, which of the following technique is not used in introducing DNA into host cell?

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