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  • Question 1
    1 / -0

    Some foreign DNA fragment is attached to Cla I site of pBR322. This recombinant vector is used to transition Escherichia coli host cells. The cells subjected to transformation are plated on two different media one containing ampicillin and the other containing tetracycline. The transformed cells containing the recombinant vector

  • Question 2
    1 / -0

    DNA polymerase for industrial use in Polymerase Chain Reaction (PCR) is produced by

  • Question 3
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    Identify the steps that are involved in PCR.
    (A) Denaturation
    (B) Annealing
    (C) Extension
    (D) Down stream processing

  • Question 4
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    Study the following lists

    List - IList II
    A)RNA  I)Cotton bollworms
    B)ELISAII)Early detection of HIV
    C)PCRIII)Meloidegyne resistance
    D)Cry I AbIV)Antigen antibody interaction
    V) Corn borer
                                          

  • Question 5
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    The production of multiple copies of DNA can be done by

    I. Autoradiography
    II. Gel electrophoresis
    III. PCR

  • Question 6
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    Restriction enzymes are used to cut

  • Question 7
    1 / -0

    A technique utilized by a group of biologists to isolate an antifreeze gene found in cold water species of fish and introduce it into the genome of a different organism is called as ?

  • Question 8
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    Identify correct combinations.

    List I
    List II
    List III
    A) PCR
    i) Specific base sequence
    I) Taq polymerase
    B) Downstream processing
    ii) Inheritable gene
    II) Ampicillin
    C) Restriction endonuclease
    iii) Amplification of DNA
    III) EcoRI
    D) Selectable marker
    iv) Transformation and non-transformation

    v) Product separation and purification
    IV) Quality control

    V) Ori

  • Question 9
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    Given below is a sample of portion of DNA strand given the base sequence on the opposite strand. What is so special show in it?
    5' - GAATTC ... 3'
    3' - CTTAAG ... 5'

  • Question 10
    1 / -0

    Which of the following would not be a valid reason to use PCR?

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