Biotechnology P...

Identify the DNA segment which is not a palindromic sequence:

PCR proceeds in three distinct steps governed by temperature. The order of the steps is 

In biotechnology application, a selectable marker is incorporated in a plasmid

Genomic DNA is digested with Alu $$I$$, a restriction enzyme which is a four base - pair cutter. What is the frequency with which it will cut the DNA assuming a random distribution of bases in the genome?

RFLP analysis

DNA polymerase required for synthesizing copies of DNA at high temperature is obtained from

During PCR technique, the pairing of primers to ssDNA segment is known as

Restriction endonucleases are isolated from some bacteria. Their role in bacteria is

A circular plasmid of $$10,000$$ base pairs (bp) is digested with two restriction enzymes, A and B, to produce a $$3000$$ bp and a $$2000$$bp bands when visualized on an agarose gel. When digested with one enzyme at a time, only one band is visible at $$5000$$ bp. If the first site for enzyme A($$A1$$) is present at the $$100^{th}$$ base, the order in which the remaining sites ($$A2, B1$$ and $$B2$$) are present is?

The restriction endonuelease EcoR-1 recognises and cleaves DNA sequence as shown below
5' - G A A T T C - 3'
3' - C T T A A G - 5'
What is the probable number of cleavage sites that can occur in a 10 kb long random DNA sequence ?