Biotechnology P...

In order to induce the bacterial uptake of plasmids, the bacteria are made competent by treating them with

Read the following statements carefully and select the correct option:
(A) Source of the restriction enzyme Hind III is E.coli
(B) In biolistic method of gene transfer, mircoparticles made up of gold or tungsten are coated with foreign DNA
(C) Micro-injection method for injecting recombinant DNA is used for animal cell
(D) Primers are chemically synthesized oligonucleotides that are complementary to the regions of DNA in PCR.
How many of the above statement are correct?

After insertion of DNA segment within the sequence of Z-gene of bacteria, they are grown on chromogenic substrate. After the growth of bacterial colonies, they are identified as
i) Recombinants if colonies are blue- coloured.
ii) Recombinants if colonies are white coloured.
iii) Non-recombinants if colonies are blue- coloured.
iv) Non-recombinants if colonies are white coloured.

Which of the following statements does not hold true for restriction enzyme ?

The technique used to make numerous copies of a specific segment of DNA quickly and accurately is

Some foreign DNA fragment is attached to Cla I site of pBR 322. This recombinant vector is used to transition Escherichia coli. The cells subjected to transformation are plated on two different media, one containing ampicillin and the other containing tetracycline. The transformed cells containing the recombinant vector will

Polymerase chain reaction (PCR) is used for

Which of the following DNA sequences qualifies to be designated as a palindrome?

If the initial number of template DNA molecules in a PCR reaction is 1000 , the number of product DNA molecules at the end of 20 cycles will be closest to 

Plants are more readily manipulated by genetic engineering than are animals because