Biotechnology P...

The given figure is the diagrammatic representation of the E. coli vector pBR322. Which of these genes can act as selectable marker?

Select the correct option to fill up the blanks.

The thermostable enzymes, 'Taq' and 'Pfu', isolated from thermophilic bacteria are.

Which of the following techniques is used to make numerous copies of a specific segment of DNA quickly and accurately?

In PCR, short primers are added to single stranded DNA molecules in a test tube and the appropriate enzymes are included to make a copy of the DNA. Which of the following primers is needed for copying the single stranded DNA sequence $$ 5'TACGGTAGGTC3'$$ ?

Palimdromic DNA sequence for EcoRI is 

A certain purified DNA sample was cut with two restriction endonucleases $$E1$$ and $$E2$$. The following results were obtained from agarose gel electrophoresis
Sample cut with $$E1$$ alone: two bands of size $$35\ kb$$ and $$15\ kb$$
Sample cut with $$E2$$ alone: two bands of size $$40\ kb$$ and $$10\ kb$$
Sample cut simultaneously with $$E1$$ and $$E2$$: three bands of size $$35\ kb, 10\ kb$$ and $$5\ kb$$
From these data, it can be inferred that the DNA has

Match the entries in Column -I with those of Column - II and choose the correct answer.

A circular plasmid of 10,000 base pairs (bp) is digested with two restriction enzymes, A and B, to produce a 3000 bp and a 2000 bp band when visualized on an agarose gel. When digested with one enzyme at a time, only one band is visible at 5000 bp. If the first site for enzyme A (A1) is present at the $$100^{th}$$ base, the order in which the remaining sites (A2, B1, and B2) are present is

Which of the following steps is/are catalysed by Taq polymerase in a PCR?