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Biotechnology and its Applications Test - 24

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Biotechnology and its Applications Test - 24
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  • Question 1
    1 / -0
    Animal suffering during testing can be minimized by which of these principles of ethics of animal biotechnology?
    Solution
    Refinement refers to methods that minimise the pain, suffering, distress or lasting harm that may be experienced by research animals, and which improve their welfare. 
    Refinement applies to all aspects of animal use, from their housing and husbandry to the scientific procedures performed on them.
  • Question 2
    1 / -0
    Which of the following is the ethics for the research with animals?
    Solution
    Anesthesia enables a animals to tolerate surgical procedures that would otherwise inflict unbearable pain.
    Bioscience community accepts that animals should be used for research only within an ethical framework.
  • Question 3
    1 / -0
    Which of the following is used as probiotic?
    Solution
    Lactobacillus acidophilus is a type of bacteria found in your intestines.
     Lactobacilli, particularly L. acidophilus, are often used as probiotics. 
    The World Health Organization defines probiotics as "live micro-organisms which, when administered in adequate amounts, confer a health benefit on the host."
  • Question 4
    1 / -0
    Which of the following enzyme used in dairy industry is produced by GM microorganism?
    Solution
    Chymosin, commonly known as rennin, is the principal milk- coagulating enzyme present in rennet. Rennet, which has a long and extensive history of safe use in making cheese and other dairy products.
  • Question 5
    1 / -0
    In gene therapy, DNAase is used to treat
    Solution
    DNase is an experimental drug used to treat cystic fibrosis. It is an autosomal recessive disease that affects lung, liver, pancreas, kidneys and intestine. It is caused due to the mutations in both copies of the gene for the cystic fibrosis transmembrane conductance regulator (CFTR). It was found that the mucus of the patient is full of DNA, which spills out of white blood cells. DNase cuts the DNA in the mucus. It is delivered in the form of an aerosol in the patient and it improves lung function by breaking up the thick mucus in the lungs. The patient is then able to clear the lungs by coughing.
    So, the correct answer is option A.
  • Question 6
    1 / -0
    Which of the following enzyme is used in ELISA?
    Solution
    ELISA is a biochemical technique used mainly in immunology to detect the presence of an antibody or an antigen in a sample. The horseradish peroxidase (HRP) and alkaline phosphatase (AP) are usually used in this technique.
    So, the correct answer is 'Both A and B'
  • Question 7
    1 / -0
    If the fermentation of molasses during the manufacturing of ethyl alcohol is delayed then the conclusion that can drawn is
    Solution
    • The sugarcane molasses are generally used as the fermentation medium. 
    • The yeast cells are grown in this medium to perform anaerobic respiration which produces ethanol and carbon dioxide. 
    • The molasses which are used is diluted so that the activity of yeast cells is fast and the production of ethanol occurs at a rapid pace. 
    • If the process is slowed down, then the molasses must not have been diluted before the addition of the fermentation medium in the fermentor.
    • Thus, the correct answer is option B. 
  • Question 8
    1 / -0
    Which of the following is a term used for the specific substance whose presence is being quantitatively or qualitative analysed?
    Solution
    Analyte is the compound or the molecule which is to be identified. The experiments are carried out in order to detect the presence of the analyte. The techniques can also be performed to measure the amount of the analyte. The substance which is known as analyte can be qualitatively or quantitatively analysed.  
    Thus, the correct answer is option B. 
  • Question 9
    1 / -0
    Which of the following technique is given in the following diagram?

    Solution
    The method of ELISA is used for the detection of the disease using the antigen and antibody interaction. In indirect ELISA, the specific antigen is coated to the wells of the ELISA plate. The sample which contains the antibody are added to the well. If there is specific (primary) antibody, they will bind to the antigen which is coated.  The secondary antibody attached to the enzyme binds to the primary antibody. The chromogenic substrate is added which is acted upon by the enzyme and the colour is produced which can be read and detected by the ELISA reader. 
    Thus, the correct answer is option B. 
  • Question 10
    1 / -0
    While purchasing a sealed packet of ghee, one should look for which mark?
    Solution
    • AGMARK is a certification mark employed on agricultural products in India. 
    • Assuring that they conform to a set of standards approved by the Directorate of Marketing and Inspection. 
    • All agricultural products like grain, milk, dairy product, etc. come under this quality assurance policy. 
    • Ghee is a dairy product and it belongs to agriculture production so it should have AGMARK.
    • So, the answer is option B.
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