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Biotechnology and its Applications Test - 25

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Biotechnology and its Applications Test - 25
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Weekly Quiz Competition
  • Question 1
    1 / -0
    The antibody which is mainly present in the sandwich ELISA is 
    Solution
    In sandwich ELISA test an antigen that is to be quantified present double epitopes, to which both monoclonal or polyclonal antibodies can bind. A monoclonal antibody helps specific detection of antigen while the polyclonal antibody captures the antigen. 
    So, the correct answer is 'All of the above'
  • Question 2
    1 / -0
    A person suffering from E.coli infection can trace the level of enterotoxin by which of the following technique?
    Solution
    The person who is suffering from Escherichia coli infection, the bacterium produces enterotoxin which acts as the antigen. The method of ELISA is used for the detection of the disease using the antigen and antibody interaction. The method can be used for the detection of the enterotoxin or the antibodies which are produced against the antigen by taking the appropriate sample from the patient's body. 
    Thus, the correct answer is option B. 
  • Question 3
    1 / -0
    Which of the following phenomena can be related to ELISA?
    Solution
    The method of ELISA is used for the detection of the disease using the antigen and antibody interaction. The reaction between the antigen and antibody is known as the agglutination reaction. This reaction is the basic principle in the diagnosis of the disease like AIDS. 
    Thus, the correct answer is option A. 
  • Question 4
    1 / -0
    The competitive indirect ELISA differs from direct ELISA, as in competitive indirect ELISA
    Solution
    In direct ELISA the presence of an antigen is analyzed, and only a labelled primary antibody is used while in competitive ELISA antigen concentration is measured by noting the extent of the signal reduction. Thus, if the detection antibody is labelled, then this would be a direct competition ELISA and if unlabelled, then this would be an indirect competition ELISA.
    So, the correct answer is option A.
  • Question 5
    1 / -0
    Which of the following disease can be detected by ELISA?
    Solution

  • Question 6
    1 / -0
    Serum antibody concentration can be detected by
    Solution

  • Question 7
    1 / -0
    Which of the following is the application of ELISA?
    Solution
    • The method of ELISA is used for the detection of the disease using the antigen and antibody interaction. 
    • The method can be used for the detection of the antibodies which are produced against the Mycobacterium from the sample of the person infected with tuberculosis. 
    • The presence of the rotaviruses in the feces can also be detected in which the viral particles act as the antigens. 
    • The presence of hepatitis B markers in the serum can also be detected. 
    Thus, the correct answer is option D. 
  • Question 8
    1 / -0
    Which of the following criteria must be considered while selecting enzyme for the ELISA?
    Solution
    The method of ELISA is used for the detection of the disease using the antigen and antibody interaction. The secondary antibody is conjugated to the enzyme which can act on the chromogenic substrate. The enzyme should be pure to react on the substrate, sensitive to give accurate results and stable. If these qualities are not maintained properly, the results which are obtained will not be accurate and proper.
    Thus, the correct answer is option D. 
  • Question 9
    1 / -0
    Which of the following technique is demonstrated in the above image.

    Solution
    The method of ELISA is used for the detection of the disease using the antigen and antibody interaction. In sandwich ELISA, the antibody is coated to the wells of the ELISA plate. If there is specific (primary) antibody, they will bind to the antigen which is added. The secondary antibody attached to the enzyme binds to the primary antibody. The chromogenic substrate is added which is acted upon by the enzyme and the colour is produced which can be read and detected by the ELISA reader. 
    Thus, the correct answer is option C. 
  • Question 10
    1 / -0
    Inhibitory antigen is present in which of the following technique?
    Solution
    The method of ELISA is used for the detection of the disease using the antigen and antibody interaction. In competitive ELISA, the antigen in the sample and the antibody are incubated. The antigen-antibody mixture is added to the well in which there are other antigens. The previously bound antibodies will be washed off. The secondary antibody conjugated with the enzyme is used for the detection of the colour. The intensity of the colour is inversely proportional to the antigen which needs to be measured.
    Thus, the correct answer is option C. 
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