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Biotechnology and its Applications Test - 32

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Biotechnology and its Applications Test - 32
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  • Question 1
    1 / -0
    Human insulin contains how many amino acids on B chain?
    Solution
    Insulin is a protein which is made up of two polypeptide chains. The two polypeptide chains are designated as A and B. A chain has 21 amino acid residues and B chain has 30 amino acid residues. The two chains are linked by disulphide bonds.
    So, the correct answer is C.

  • Question 2
    1 / -0
    Proinsulin and insulin differ in structure due to the presence of the _____ in proinsulin.
    Solution
    • Proinsulin is a single polypeptide chain composed of the B and A subunits of insulin joined by the C-peptide region. 
    • It is converted into proinsulin by the peptidase enzyme. It removes C-peptide from its N-terminus and later A and B polypeptides are joined together by two disulphide bonds.

    So, the correct answer is option C.

  • Question 3
    1 / -0
    Due to .......... therapy changes would be heritable.
    Solution
    Gene therapy is treating the diseases by modifying the gene expression or inserting the deficient gene therapeutically into the patients cells. Gene therapy is of two types, somatic gene therapy and germline gene therapy.
    In somatic gene therapy, gene manipulation is done in the cells which will affect the patient but it will not be inherited. In germline gene therapy, gene manipulation is done in the germ cells or the gametes that will be used to conceive. The modified germ cells will produce the gametes having the inserted gene and on fertilisation will produce a zygote that will form the individual in which each and every cell will have the manipulated gene, because every cell descendes from the zygote. Thus, the changes will be heritable.
    So, the correct answer is C.
  • Question 4
    1 / -0
    What is searched from the patient's blood in ELISA method for the detection of AIDS?
    Solution
    • ELISA or Enzyme-linked Immunosorbent Assay is a technique to detect the presence of antigen or antibody in the given sample. It is based on antigen-antibody reaction. 
    • ELISA can detect antibodies related to infectious diseases. That's why ELISA is used as a confirmatory test for AIDS. 
    • The known antigens are immobilized on the surface of wells in a microtiter plate. Then the sample in which the presence of antibodies has to be tested is added. 
    • There occurs antigen-antibody reaction and produces a colour that confirms the presence of the antibodies.
    So, the correct answer is B.
  • Question 5
    1 / -0
    ..........is the transfer of normal genes into body cells to correct a genetic defect.
    Solution
    Gene therapy is a collection of methods that allow correction of a gene defect that has been diagnosed in a child/embryo. In this method, genes are inserted into a person's cells and tissues to treat a disease. Correction of a genetic defect involves delivery of a normal gene into the individual or embryo to take over the function of and compensate for the non-functional gene.
    So, the correct option is 'gene therapy'.
  • Question 6
    1 / -0
    ELISA test is used for the detection of
    Solution
    An enzyme-linked immunosorbent assay also called ELISA that is the test that detects and measures antibodies in the blood. This test can be used to determine if one has antibodies related to certain infectious conditions. An ELISA test may be used to diagnose HIV and STD.
    So, the correct option is 'AIDS, STD, and hepatitis'.
  • Question 7
    1 / -0
    Which does not describe a function of the DNA polymerase molecule?
    Solution
    DNA polymerase is an enzyme that synthesizes DNA molecules from deoxyribonucleotides, the building blocks of DNA. The function of DNA polymerase is to replicate, proofread and repair DNA.DNA polymerases add nucleotide bases only when an RNA primer, a short piece of RNA, is already present. 
    So, the correct option is 'Make the proper nucleotide to match with the base read on the template strand'.
  • Question 8
    1 / -0
    What is the function of the polymerase chain reaction in genetic engineering?
    Solution
    Polymerase Chain Reaction is a method widely used to make many copies of a specific DNA segment. Using PCR, a single (or more) copy of a DNA sequence is exponentially amplified to generate thousands to millions of more copies of that particular DNA segment.
    So, the correct option is 'Make millions of copies of a specific segment of DNA'.
  • Question 9
    1 / -0
    The method of correcting the defective genes is called
    Solution
    The use of bioengineered cells and biological techniques to treat human genetic disorder is known as gene therapy. Gene therapy is the transfer of a normal gene into body cells to correct the genetic defect. It can be used to treat genetic diseases like sickle cell anaemia and SCID.
    The SCID patients have no functioning t lymphocytes and one treated with injections of the white blood cells that have been engineered to carry the normal ADA alleles.
    So, the correct option is 'gene therapy'
  • Question 10
    1 / -0
    Which of the following is an improved variety of wheat crop?
    Solution
    Roja-64 A, Sonalika, Sonara-64 all are improved variety of wheat crop.
    So, the correct option is 'All of these'
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