Biotechnology and its Applications Test

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Biotechnology and its Applications Test - 38

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Weekly Quiz Competition

Question 1
1 / -0

Peptide A and peptide B is linked by how many disulfide linkages in insulin?

A
$$1$$

B
$$2$$

C
$$3$$

D
$$4$$
Solution
- Proinsulin is made up of 81 residues and formed by three distinct chains.
- The A chain, B chain, and the area connecting the two are named the C peptide.
- The correct structure of proinsulin is crucial for the correct folding of mature insulin, as the placement of the C peptide sets the molecule up to create correctly positioned disulfide bonds in and between the A and B chains.
- There are three disulfide bonds that are necessary for mature insulin to be the correct structure. Two of these disulfide bonds are between the A and B chains, and one is an intra-A chain bond.
So the correct option is '2'.
Question 2
1 / -0

Which type of pH conditions are required for action by Bt toxin?

A
$$8.6$$

B
$$10$$

C
$$7.0$$

D
$$6.8$$

Solution

The alkaline pH (8.6 approximately) of the gut in insects triggers activation of protoxin ( inactive crystallized form) to active Bt toxin after ingestion.
Question 3
1 / -0

What is true among the following w.r.t transgenic cows?

A
The milk had a protein called human $$\alpha - lactalbumin $$

B
The 1st transgenic cow was called Rosie

C
Both A and B are correct

D
None of these

Solution

The first transgenic cow was Rosie and was produced in 1997. It carried the gene for human alpha-lactalbumin due to which the milk of the cow contained $$\alpha-lactalbumin$$.
So, the correct answer is 'Both A and B are correct.
Question 4
1 / -0

Read the following statements :
I) Mule is developed by interspecific hybridisation
II) Groups of bees is called 'swarms'
III) For the herd-improvement cross-breeding is employed
IV) In MOET, fertilised egg at 6-8 celled stage is recovered non - surgically

A
I, II, III, IV are true

B
III , IV, V are false

C
I, II, IV are true

D
I, II, III and V are true
Solution
- As a mule is produced by crossing a female horse with a male donkey, it can be said to be an interspecific hybrid.
- Swarming can be defined as the process by which a queen bee forms a new colony. Consequently, the term ‘swarms’ is used to refer to the colonies of honey bees.
- Cross-breeding enables the production of hybrids that are superior in one respect or the other to their respective parents.
- In MOET, the female is made to produce more eggs than normal, usually 6-8. After the fertilization of these eggs with sperms, these fertilized eggs are non-surgically removed and transferred to other females.
Hence, the correct answer is 'I, II, III, IV are true'.
Question 5
1 / -0

X technique is now routinely used to detect HIV in suspected AIDs patients. It is being used to detect mutations in genes in suspected cancer patients too. It is a powerful technique to identify many other genetic disorders. Identify X-

A
X=PCR

B
X=DNA fingerprinting

C
X-Bioinformatic

D
X-X-ray defreaction

Solution

The development of molecular techniques that access viral load and the development of genotypic resistance have revolutionized the treatment of HIV disease. Commercially available viral load assays use a number of different approaches from reverse transcriptase PCR to amplification of branched chain DNA. New real-time PCR assays are under development, including LightCycler- and TaqMan-based tests. So, the correct answer is option A. ( X=PCR ).
Question 6
1 / -0

Transgenic plants have been shown to express the genes of
I) Insulin
II) Interferons
III) Human growth hormones
IV) Antibodies
V) Antibiotics

A
I, II, III, IV, V

B
Except III, IV

C
Except IV

D
I, II, III only

Solution

Transgenic plants are plants that have genomes that are modified using genetic engineering techniques. So any kind of genes that can be inserted into the plants are used to express various genes for the industrial production e.g. insulin, interferons, human growth hormones, antibodies, antibiotics.
So the correct option is A
Question 7
1 / -0

Biopiracy means?

A
Use of bio-patents

B
Thefts of plants and animals

C
Stealing of bio-resources

D
Exploitation of bio-resources without authentic permission

Solution

D. Exploitation of bio-resources without authentic permission
EXPLANATION: Bio means life and piracy means stealing someone else's intellectual property. Use of someone's bioresources without any systemic permission is biopiracy.
Question 8
1 / -0

Which of the following technique is employed to check the progression of a restriction enzyme digestion?

A
PCR

B
Bioreactor

C
Gel electrophoresis

D
Micro injection

Solution

Answer is option C i.e. "Gel electrophoresis"
Gel electrophoresis is the technique which can check DNA fragment progression. Restriction enzyme digest DNA into fragments. Each fragment has different size. When gel electrophoresis is performed each fragment with different sizes are separated on the gel.
While, PCR is used for DNA amplification. Bioreactors are the fermentors used for fermentation process and micro-injection is a method used to transfer genes between animals.
Question 9
1 / -0

Assertion: Humulin synthesizes in the form of proinsulin.
Reason: C-peptide is not seen in mature insulin.

A
A and R both are correct. Where R is explanation of A

B
A and R both are correct. Where R is not explanation of A

C
A is correct white R is correct

D
A is incorrect white R is correct

Solution

Insulin in the human body is synthesized in the form of proinsulin. Proinsulin is a molecule that has a B-chain peptide, C-chain peptide, and A-chain peptide. The C-chain peptide of the precursor insulin molecule. In mature insulin, the C-chain peptide is cleaved off which results in the joining of A-chain peptide and B-chain peptide and forms its active form.
So the correct answer is ' A and R both are correct. Where R is the explanation of A '.
Question 10
1 / -0

Which of the following can't be achieved using PCR ?

A
Detect HIV in AIDS suspect

B
detect mutations in cancer patients

C
Detect antigen - antibody interaction

D
Detect specific micro organism from soil

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Re-Attempt Weekly Quiz Competition

Biotechnology and its Applications Test - 38

Result

Biotechnology and its Applications Test - 38

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SHARING IS CARING

Question 1

$$1$$

$$2$$

$$3$$

$$4$$

Solution

Question 2

$$8.6$$

$$10$$

$$7.0$$

$$6.8$$

Solution

Question 3

The milk had a protein called human $$\alpha - lactalbumin $$

The 1st transgenic cow was called Rosie

Both A and B are correct

None of these

Solution

Question 4

I, II, III, IV are true

III , IV, V are false

I, II, IV are true

I, II, III and V are true

Solution

Question 5

X=PCR

X=DNA fingerprinting

X-Bioinformatic

X-X-ray defreaction

Solution

Question 6

I, II, III, IV, V

Except III, IV

Except IV

I, II, III only

Solution

Question 7

Use of bio-patents

Thefts of plants and animals

Stealing of bio-resources

Exploitation of bio-resources without authentic permission

Solution

Question 8

PCR

Bioreactor

Gel electrophoresis

Micro injection

Solution

Question 9

A and R both are correct. Where R is explanation of A

A and R both are correct. Where R is not explanation of A

A is correct white R is correct

A is incorrect white R is correct

Solution

Question 10

Detect HIV in AIDS suspect

detect mutations in cancer patients

Detect antigen - antibody interaction

Detect specific micro organism from soil

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