Biotechnology and its Applications Test

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Biotechnology and its Applications Test - 45

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Weekly Quiz Competition

Question 1
1 / -0

In one of the techniques of recombinant insulin production the genes for $$\alpha$$ and $$\beta$$ polypeptides were inserted into the plasmid by the side of

A
Antibiotic resistance gene

B
Lac z promoter gene

C
$$\beta$$ galactosidase gene

D
Ori
Solution
- The recombinant insulin can be produced by the insertion of the genes for α and β polypeptides into a plasmid by the side of a beta-galactosidase gene which regulates the gene expression.
- The gene expresses itself after transformation into a host DNA and leads to production of recombinant insulin.
So, the correct answer is option C.
Question 2
1 / -0

Potatoes with vaccines are used to

A
Improve fatty acid and vitamin A content

B
Improve starch and vitamin A content

C
Improve starch and vitamin E content

D
None of the above

Solution

Recombinant DNA technology has developed crops that have been introduced with vaccines. These are genetically modified crops. There are many crops developed which helps to provide nourishment to the people who cannot afford fortified foods. The potato was injected with a vaccine to improve the amount of starch produced in the crop. The crop was also injected with vitamin A or derivatives which can be converted to vitamin A, like provitamin A after consumption by humans. This helps to provide food and improve the health of poor people by supplying staple foods.
Thus, the correct answer is option B.
Question 3
1 / -0

Which of the following can minimizes the ethical issues of animal biotechnology?

A
Biopatent

B
Ban on experimental use of animals

C
Create new species only for experiments

D
Both A and B
Solution
The use of biopatents stops biopiracy (use of bioresource by multinational companies and other organizations without proper authorization from the countries and individuals concerned without compensatory payment).
Ban on experimental use of animals for animal biotechnology protects animal life as using animals in biotechnology causes great suffering to them.
So, the correct answer is option D.
Question 4
1 / -0

Read the two statements A and B and identify the correct choice from the given options.
Statement A : Agrobacterium tumefaciens is the causative agent of crown gall disease of dicots.
Statement B : Agrobacterium tumefaciens cause infection by entering the plant through wounds and injuries.

A
Statement A is correct and B is wrong

B
Statement B is correct and A is wrong

C
Both statements A and B are correct

D
Both statements A and B are wrong

Solution

Agrobacterium tumefaciens is a Gram-negative bacterium. It is rod shaped bacterium. It is the causative agent of crown gall disease of dicots. They have a T-plasmid which have the tumor causing genes. This gene causes formation of tumor when the plant is infected with the bacterium. The bacterium enters the plant when there is some wound or injury caused to the plant.
Thus, the correct answer is option C.
Question 5
1 / -0

Ethics are associated with genetic engineering because of ___________.

A
Human dignity

B
Commercialization of genetic research

C
Fair use of genetic information

D
All of the above

Solution

The genetic engineering of animals has increased significantly in recent years, and the use of this technology brings with it ethical issues, some of which relate to animal welfare — defined by the World Organisation for Animal Health as “the state of the animal.
How an animal is coping with the conditions in which it lives”.
These issues need to be considered by all stakeholders, including veterinarians, to ensure that all parties are aware of the ethical issues at stake and can make a valid contribution to the current debate regarding the creation and use of genetically engineered animals.
Question 6
1 / -0

Which of the following protein is produced by the genetically modified microorganism to improve animal milk production?

A
Albumin protein

B
Bovine somatotropin

C
Bacteriocin

D
None of these

Solution

Bovine somatotropin is a growth hormone used as an animal drug to increase milk production in cows.
Thus, the correct answer is (B).
Question 7
1 / -0

Which of the following ethical issues should be considered for developing transgenic animal?

A
Yielding capacity

B
Long term effect on environment

C
Quality of breed

D
All of the above

Solution

The ethical issues considered for developing transgenic animals are yielding capacity, long term effect on the environment, quality of the breed. They include bioethicists, environmentalists. the results of genetic alteration would be suffered and hence such experimentation should be banned (Eg, a pig with no legs)
Question 8
1 / -0

The enzymatic degradation of lactose in dairy product can reduce the__________.

A
Casein

B
Whey

C
Microbes

D
Acrylamide

Solution

Acrylamide does not form, or forms at lower levels, in dairy, meat, and fish products. so the enzymatic degradation of lactose in dairy produts can reduce the acrylamide.
Question 9
1 / -0

Which of the following GM microbe contains gene for naphthalene?

A
Mycobacterium tuberculosis

B
E.coli

C
Pseudomonas putida

D
Pseudomonas aeruginosa
Question 10
1 / -0

Arrange the following steps of ELISA in sequential order.
a) Addition of substrate in the linked enzyme.
b) Attachment of antibody conjugated with an enzyme.
c) Coating of virus sample on the surface.

A
a), c), b)

B
a), b), c)

C
c), b), a)

D
c), a), b)

Solution

The method of ELISA is used for the detection of the disease using the antigen and antibody interaction. The specific antigen or virus particle is coated to the wells of the ELISA plate. If there is specific (primary) antibody in the sample, they will bind to the antigen which is coated. The secondary antibody attached to the enzyme binds to the primary antibody. The chromogenic substrate is added which is acted upon by the enzyme and the colour is produced which can be read and detected by the ELISA reader.
Thus, the correct answer is option C.

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Re-Attempt Weekly Quiz Competition

Biotechnology and its Applications Test - 45

Result

Biotechnology and its Applications Test - 45

Score

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Rank

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SHARING IS CARING

Question 1

Antibiotic resistance gene

Lac z promoter gene

$$\beta$$ galactosidase gene

Ori

Solution

Question 2

Improve fatty acid and vitamin A content

Improve starch and vitamin A content

Improve starch and vitamin E content

None of the above

Solution

Question 3

Biopatent

Ban on experimental use of animals

Create new species only for experiments

Both A and B

Solution

Question 4

Statement A is correct and B is wrong

Statement B is correct and A is wrong

Both statements A and B are correct

Both statements A and B are wrong

Solution

Question 5

Human dignity

Commercialization of genetic research

Fair use of genetic information

All of the above

Solution

Question 6

Albumin protein

Bovine somatotropin

Bacteriocin

None of these

Solution

Question 7

Yielding capacity

Long term effect on environment

Quality of breed

All of the above

Solution

Question 8

Casein

Whey

Microbes

Acrylamide

Solution

Question 9

Mycobacterium tuberculosis

E.coli

Pseudomonas putida

Pseudomonas aeruginosa

Question 10

a), c), b)

a), b), c)

c), b), a)

c), a), b)

Solution

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