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Biotechnology Test - 1

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Biotechnology Test - 1
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  • Question 1
    1 / -0

    EtBr fluoresces at the wavelength

    Solution

    Ethidium bromide is an intercalating agent commonly used as a fluorescent tag (nucleic acid stain) in molecular biology laboratories for techniques such as agarose gel electrophoresis. It is commonly abbreviated as "EtBr", which is also an abbreviation for bromoethane. When exposed to ultraviolet light, it will fluoresce with an orange colour, intensifying almost 20-fold after binding to DNA. It fluoresces at the wavelength at 254 nm.

     

  • Question 2
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    OCT, XAM and CAM are

  • Question 3
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    Which of the following statements is true about complementary DNA (cDNA)?

  • Question 4
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    The following question has four choices out of which ONLY ONE is correct.

    What is antisense technology?

  • Question 5
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    Shotgun technique is employed in

  • Question 6
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    Protein encoding gene expressed in heterologous host is called as

    Solution

    Recombinant Protein is a protein encoded by a gene — recombinant DNA — that has been cloned in a system that supports expression of the gene and translation of messenger RNA (see expression system). Modification of the gene by recombinant DNA technology can lead to expression of a mutant protein. Protein encoding genes are commonly described as structural or regulatory. Structural gene encodes enzymes and structural protein.

     

  • Question 7
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    The enzyme used to cut the DNA molecule at specific site is

  • Question 8
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    The techniques of using live organisms of enzymes from organism to produce products and processes useful to human is called

    Solution

    Biotechnology is the use of living systems and organisms to develop or make products, or "any technological application that uses biological systems, living organisms, or derivatives thereof, to make or modify products or processes for specific use".

     

  • Question 9
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    PCR based markers have been developed to

  • Question 10
    1 / -0

    Yeast-two hybrid system is used to study

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