Biotechnology Test

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Biotechnology Test - 3

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Weekly Quiz Competition

Question 1
1 / -0.25

Origin of replication is the specific DNA sequence on chromosome that which is responsible for:

A
Initiating replication.

B
Synthesis of polymerase enzyme.

C
Terminating replication.

D
All of the these.
Solution
Origin of replication is the specific DNA sequence on chromosome that which is responsible for initiating replication.

Origin of replication is essential for replication of particular DNA segment.
Question 2
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The group of letters that form same words when read both forward and backward are called?

A
Palindrome

B
Puzzle

C
Endonucleases

D
Sticky ends

Solution

The groups of letters that form same words when read both forward and backward are called Palindrome. For example MALYALAM which read same from both side.
Question 3
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Enzymes used to join foreign DNA to plasmid are _________.

A
Nucleases

B
Ligases

C
Endonucleases

D
Pectinases

Solution

The Enzymes used to join foreign DNA to plasmid is ligases. The plasmid of bacteria replicate this DNA strands along with other which can be further transferred to target cells.
Question 4
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Which of the given statements is correct in the context of visualizing DNA molecules separated by agarose gel electrophoresis?

A
Ethidium bromide stained DNA can be seen under exposure to UV light.

B
DNA can be seen without staining in visible light.

C
Ethidium bromide stained DNA can be seen in visible light.

D
DNA can be seen in visible light.

Solution

The separated DNA fragments can he visualised only after staining the DNA with a compound known as ethidium bromide followed by exposure to ultraviolet radiation (we cannot see pure DNA fragments in the visible light and without staining).
Question 5
1 / -0.25

‘R ’in EcoRI restriction endonuclease denotes:

A
Strain RY13

B
Sequence of isolation of the enzyme

C
Species

D
Genus

Solution

EcoRI comes from Escherichia coli RY 13. In EcoRI, the letter ‘R ’is derived from the name of strain.
Question 6
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Assertion: The separated DNA fragments can be visualized only after staining the DNA with a compound known as ethidium bromide followed by exposure to UV radiation.
Reason: We can see bright red coloured bands of DNA in an ethidium bromide-stained gel exposed to UV light.

A
Both assertion and reason are correct.

B
Assertion is correct but reason is incorrect.

C
Both assertion and reason are incorrect.

D
Assertion is correct but reason does not explain the assertin.

Solution

The separated DNA fragments can be visualized only after staining the DNA with a compound known as ethidium bromide followed by exposure to UV radiation. We can see bright orange coloured bands of DNA in aethidium bromide stained gel exposed to UV light.
Question 7
1 / -0.25

DNA fragments move towards the anode during gel electrophoresis. This is because _________.

A
Anode is negatively charged.

B
DNA moves in random direction.

C
DNA is negatively charged molecules.

D
DNA is positively charged molecules.
Solution
In gel electrophoresis, DNA molecules moves towards positively charged anode because DNA is negatively charged molecules.

The molecules separate out due to difference in molecular weight.
Question 8
1 / -0.25

Rop genes in pBR322 codes for _________.

A
Tetracycline resistance.

B
Antibiotic resistance.

C
Proteins involved in replication of plasmid.

D
Ampicillin resistance.
Solution
Rop genes in pBR322 codes for protein involved in replication of plasmid.

Plasmid are able to take the foreign gene and to be transferred to target cells.
Question 9
1 / -0.25

The particles used to coat with DNA in Biolistic gun is of:

A
Tungsten

B
Zinc

C
Helium

D
Quartz

Solution

DNA is coated with tungsten before used in biolistic gun for inserting the DNA directly into target cells for obtaining particular protein.
Question 10
1 / -0.25

Production of large scale recombinant products can be done in _________.

A
Tissue culture labs

B
Bioreactors

C
Autoclave

D
Thermocycler

Solution

Bioreactor is used to produce large scale production. Bioreactor is a large vessels in which large scale raw materials are biologically converted into specific products.
Question 11
1 / -0.25

Sparged stirred tank bioreactor is advantageous over the simply stirred tank bioreactor as _________.

A
It requires less of maintenance.

B
Sparged tank has blades which move fast.

C
Air bubbles enhance the oxygen transfer area.

D
Vitamins and minerals are more used up in sparged stirred bioreactor.
Solution
Bioreactors are used to obtain biological products on large scale continually for commercial purpose.

The sparged stirred tank bioreactor is advantageous over the simple stirred tank bioreactor as air bubbles enhance the oxygen transfer area.
Question 12
1 / -0.25

The X-gal will be converted into a coloured product when:

A
When lactose is available.

B
When goi is inserted in the vector at the site coding for B-galactosidase.

C
Gene coding for B-galactosidase is cleaved.

D
B-galactosidase acts on it.

Solution

The X-gel will be converted into a coloured product when B-galactosidasse acts on it.
Question 13
1 / -0.25

Blades in a bioreactor help in?

A
Provides air bubbles.

B
Provides ample space for the formation of recombinant protein product.

C
Provides energy to bacterial cells.

D
Mixing of all the components and prevent their settling.
Solution
Blades in a bioreactor help in mixing of all the components and prevent their settling at the bottom of reactor tank.

It also increases the oxygen level in bioreactor.
Question 14
1 / -0.25

The restriction sites of the nucleoid are methylated because:

A
Methylation helps in easy cutting by the molecular scissors.

B
To protect bacteria from its own enzymes.

C
Methylation helps in easy transfer of DNA.

D
Methylation is important for the identification by restriction enzymes.

Solution

The restriction sites of the nucleoid are methylated because it protects bacteria from its own enzymes.
Question 15
1 / -0.25

A ladder is used in Gel electrophoresis as it helps in _________.

A
It helps in denaturation of DNA.

B
It helps in EtBr binding.

C
Comparing the size of the DNA fragment.

D
It prevents the movement of DNA out of the gel.
Solution
A ladder is used in Gel electrophoresis as it helps in comparing the size of the DNA fragment.

The fragments get separated from each other on the basis of molecular weight of the fragments.

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Biotechnology Test - 3

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Biotechnology Test - 3

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SHARING IS CARING

Question 1

Initiating replication.

Synthesis of polymerase enzyme.

Terminating replication.

All of the these.

Solution

Question 2

Palindrome

Puzzle

Endonucleases

Sticky ends

Solution

Question 3

Nucleases

Ligases

Endonucleases

Pectinases

Solution

Question 4

Ethidium bromide stained DNA can be seen under exposure to UV light.

DNA can be seen without staining in visible light.

Ethidium bromide stained DNA can be seen in visible light.

DNA can be seen in visible light.

Solution

Question 5

Strain RY13

Sequence of isolation of the enzyme

Species

Genus

Solution

Question 6

Both assertion and reason are correct.

Assertion is correct but reason is incorrect.

Both assertion and reason are incorrect.

Assertion is correct but reason does not explain the assertin.

Solution

Question 7

Anode is negatively charged.

DNA moves in random direction.

DNA is negatively charged molecules.

DNA is positively charged molecules.

Solution

Question 8

Tetracycline resistance.

Antibiotic resistance.

Proteins involved in replication of plasmid.

Ampicillin resistance.

Solution

Question 9

Tungsten

Zinc

Helium

Quartz

Solution

Question 10

Tissue culture labs

Bioreactors

Autoclave

Thermocycler

Solution

Question 11

It requires less of maintenance.

Sparged tank has blades which move fast.

Air bubbles enhance the oxygen transfer area.

Vitamins and minerals are more used up in sparged stirred bioreactor.

Solution

Question 12

When lactose is available.

When goi is inserted in the vector at the site coding for B-galactosidase.

Gene coding for B-galactosidase is cleaved.

B-galactosidase acts on it.

Solution