Biotechnology Test - 6

Biotechnology is the techniques of using live organisms of enzymes from organism to produce products and processes useful to human.

Ethedium bromide fluoresces at the wavelength at 254 nm.

Phage is a virus that infects bacteria. Such viruses transfer their genetic materials into host bacteria and using the bacterial machinery increase in number and multiply rapidly to kill host cells.

Plasmid is a small DNA molecule within a cell that is physically separated from a chromosomal DNA and can replicate independently. Plasmid is most commonly found in bacteria as small circular, double stranded DNA molecule.

In this technique,  the desired DNA is linked to the virus or plasmid to make the multiple copy of desire substance in host body.

Restriction enzymes belongs to a larger class of enzymes called nucleases. Which are of two kinds endonucleases and exonucleases.

The extraction of DNA from the agrose gel is called as elution. There are many methods for eluting DNA from a piece of agrose.

Protein can be digested by using enzyme protease to obtain DNA. In chromosome DNA is wrapped on histone protein.

The enzyme nuclease acts on nucleic acid DNA and RNA. This enzyme is used to hydrolyze nucleic acid.

Protein molecules are smaller than DNA polynucleotide. Protein and DNA can be separated by polyacrylamide gel having minute pores through which protein can pass but not the DNA.