Molecular Basis of Inheritance Test

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Molecular Basis of Inheritance Test - 2

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Weekly Quiz Competition

Question 1
4 / -1

Which of the following phenomena was experimentally proved by Meselson and Stahl?

A
Transformation

B
Transduction

C
Semi-conservative DNA replication

D
Central dogma

Solution

The Meselson and Stahl experiment was an experiment to prove that DNA replication was semi-subsequently and it was first shown in Escherichia coli and subsequently in higher organisms, such as plants and human cells. Semi-conservative replication means that when the double stranded DNA helix was replicated, each pf the two double stranded DNA helices consisted of one strand coming from the parental helix and one is newly synthesised.
Question 2
4 / -1

To prove that DNA is the genetic material, which radioactive isotopes were used by Hershey and Chase(1952) in their experiments?

A
³⁵S and ¹⁵N

B
³²P and ³⁵S

C
³²P and ¹⁵N

D
¹⁴N and ⁵N

Solution

Hershey and Chase experiment is based on the fact that DNA contains phosphorus and similarly, sulphur is present in proteins but not in DNA. They incorporated radioactive isotope of phosphorus (³²P) into phage DNA and that of sulphur (³⁵S) into proteins of a separate phage culture. Viruses grown in the presence of radioactive phosphorus contained radioactive DNA but not radioactive protein because DNA contains phosphorus but proteins do not, Similarly, viruses grown on radioactive sulphur contain radioactive- protein but not-radioactive DNA because DNA does not contain sulphur.
Question 3
4 / -1

Choose the correct answer from the alternatives given:
Watson and Crick (1953) proposed DNA double helix model and won the Nobel Prize; their model of DNA was based on
(i) X-ray diffraction studies of DNA done by Wilkins and Franklin.
(ii) Chargaffs base equivalence rule.
(iii) Griffiths transformation experiment.
(iv) Meselson and Stahls experiment.

A
(i), (ii) and (iv)

B
(i) and (ii)

C
(iii) and (iv)

D
(i), (ii), (iii) and (iv)

Solution

In 1953, James Watson and Francis Crick based on the X-ray diffraction data produced by Maurice Wilkins and Rosalind Franklin, proposed double helix model for the structure of DNA. One of the hallmarks of their proposition was base pairing between two strands of polynucleotide chains. However, this proportion was also based on the observation of Erwin Chargaff (1950) that for a double stranded DNA, the ratios between Adenine and Thymine and Guanine and Cytosine are constant and equals one.
Question 4
4 / -1

Q. The process of transformation is not affected by which of the following enzymes?
A. DNase
B. RNase
C. Peptidase
D. Lipase

A
A, B

B
A, B, C, D

C
B, C, D

D
A, B, C

Solution

Transformation is a phenomenon by which DNA isolated from one type of cell when introduced into another type is able to give some of its properties to the latter. In 1944, Avery, McCarty and MacLeod discovered that protein- digesting enzymes (proteases) and RNA-digesting enzymes (RNases) did not affect transformation, so the transforming substance was not a protein or RNA. Digestion with DNase did inhibit transformation, suggesting that the DNA caused the transformation. They concluded that DNA is the hereditary material.
Question 5
4 / -1

Choose the correct answer from the alternatives given:
Which of the following criteria should be fulfilled by a molecule to act as a genetic material?
(i) It should be able to replicate.
(ii) It should be structurally and chemically stable.
(iii) It should be able to undergo slow mutations.
(iv) It should be able to express itself in the form of Mendelian characters.

A
(i) and (ii)

B
(ii) and (iii)

C
(i), (ii) and (iii)

D
(i), (ii), (iii) and (iv)

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Re-Attempt Weekly Quiz Competition

Molecular Basis of Inheritance Test - 2

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Molecular Basis of Inheritance Test - 2

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Question 1

Transformation

Transduction

Semi-conservative DNA replication

Central dogma

Solution

Question 2

35S and 15N

32P and 35S

32P and 15N

14N and 5N

Solution

Question 3

(i), (ii) and (iv)

(i) and (ii)

(iii) and (iv)

(i), (ii), (iii) and (iv)

Solution

Question 4

A, B

A, B, C, D

B, C, D

A, B, C

Solution

Question 5

(i) and (ii)

(ii) and (iii)

(i), (ii) and (iii)

(i), (ii), (iii) and (iv)

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³⁵S and ¹⁵N

³²P and ³⁵S

³²P and ¹⁵N

¹⁴N and ⁵N