Biotechnology: Principle & Processes Test

Question 1
4 / -1

Stirred-tank bioreactors have advantages over shake flasks because they ___________.

A
Provide high temperature and pH

B
Provide better aeration and mixing properties

C
Do not allow the entry of CO₂

D
Are easy to operate

Question 2
4 / -1

The different steps of recombinant DNA technology are given below randomly.
(i) Isolation of the DNA fragments or genes to be cloned
(ii) Introduction of the recombinant DNA into a suitable cell (usually E.coli) called host (transformation)
(iii) Multiplication/expression of the introduced gene in the host
(iv) Selection of the transformed host cells, and identification of the clone containing the desired gene/DNA fragment
(v) Insertion of the isolated gene in a suitable plasmid vector
Which of the following represents the correct sequence of steps?

A
(i), (iii), (ii), (iv), (v)

B
(iii), (ii), (i), (v), (iv)

C
(i), (v), (ii), (iv), (iii)

D
(v), (i), (iii), (iv), (ii)

Question 3
4 / -1

Given table gives an account of differences between PCR and gene cloning.. Which of the following points show the incorrect differences?

A
1 and 3

B
4 and 6

C
4 and 7

D
4, 7 and 8

Question 4
4 / -1

Primers are

A
Chemically synthesised oligonucleotides that are complementary to the regions of DNA

B
Chemically synthesised oligonucleotides that are not complementary to the regions of DNA

C
Chemically synthesised, autonomously replicating circular DNA molecules

D
Specific sequences present on recombinant DNA

Question 5
4 / -1

Enzyme' Taq polymerase' used in PCR, has been isolated from bacterium ________.

A
Agrobacterium tumefaciens

B
Thermus aquaticus

C
Streptomyces albus

D
Escherichia coli

Question 6
4 / -1

In addition to the Taq polymerase enzyme, which other thermostable DNA polymerases have been isolated to be used in a polymerase chain reaction (PCR)?

A
Pfu polymerase isolated from Pyrococcus furiosus

B
Tli polymerase (vent polymerase) isolated from Thermococcus litoralis

C
Both (a) and (b)

D
None of these

Question 7
4 / -1

After completion of the biosynthetic stage in the bioreactors, the product undergoes separation and purification processes, collectively termed as __________.

A
Transformation

B
Electrophoresis

C
Downstream processing

D
Upstream processing

Question 8
4 / -1

During isolation of genetic material, the chemical used to precipitate out the purified DNA is

A
Bromophenol blue

B
Chilled ethanol

C
Ethidium bromide

D
Both A and C

Question 9
4 / -1

The given flow chart depicts the steps to transfer a desirable gene of interest into a plant.
Identify the missing steps (A, B and C) with regard to following statements and select the correct option.
(i) Joining of desirable gene to a suitable cloning vector using ligases to create a recombinant DNA molecule
(ii) Selection of transformed cells
(iii) Transferring the recombinant DNA molecules to the target cells

A
A - i, B - ii, C - iii

B
A - i, B - iii, C - ii

C
A - ii, B - iii, C - i

D
A - iii, B - i, C - ii

Question 10
4 / -1

Eukaryotic genes do not function properly when cloned into a bacterial cell because

A
of the inability to excise introns and destruction by bacterial restriction enzymes

B
of high pH present in bacterial cells

C
of inappropriate insertion of genes

D
both (a) and (b)

Question 11
4 / -1

Match column I with column II and select the correct answer from the given codes.

A
A-(iv), B-(i), C-(iii), D-(ii)

B
A-(i), B-(iii), C-(ii), D-(iv)

C
A-(ii), B-(i), C-(iii), D-(iv)

D
A-(iv), B-(ii), C-(i), D-(iii)

Question 12
4 / -1

Study the following statements regarding recombinant DNA technology and select the incorrect ones
(i) Taq polymerase extends the primers using the nucleotides provided in the reaction
(ii) Antibiotic resistance genes are considered as desirable genes in recombinant DNA technology
(iii) DNA fragments are separated according to their charge only, in agarose gel electrophoresis
(iv) Transformation is a procedure through which a piece of DNA is integrated into the genome of a host bacterium
(v) To produce higher yields of the desired protein, host cells can be multiplied in a continuous culture
(vi) Downstream processing is one of the steps of polymerase chain reaction

A
(i), (iii) and (vi)

B
(i), (iii) and (v)

C
(ii), (iii) and (v)

D
(i), (iv) and (v)

Question 13
4 / -1

Which of the following are the types of bioreactors?
(i) Simple stirred-tank bioreactor
(ii) Complex Stirred-tank bioreactor
(iii) Sparged stirred-tank bioreactor
(iv) Agitator stirred-tank bioreactor

A
(i) and (iii)

B
Only (iii)

C
(i) and (ii)

D
(i) and (ii) and (iv)

Question 14
4 / -1

Read statements (i)-(iv). Which of the following statements are incorrect?
(i) First transgenic buffalo Rosie produced milk which was human alpha-lactalbumin enriched.
(ii) Restriction enzymes are used in isolation of DNA from other macromolecules.
(iii) Downstream processing is one of the steps of rDNA technology.
(iv) Disarmed pathogen vectors are also used in the transfer of rDNA into the host.

A
(ii) and (iii)

B
(iii) and (iv)

C
(i) and (iii)

D
(i) and (ii)

Question 15
4 / -1

An advantage of using yeasts rather than bacteria as recipient cells for the recombinant DNA of eukaryotes is that yeasts can __________.

A
Produce restriction enzymes

B
Excise introns from the RNA transcript

C
Remove methyl groups

D
Reproduce more rapidly

Submit Test

Biotechnology: ...

Question 1

Provide high temperature and pH

Provide better aeration and mixing properties

Do not allow the entry of CO2​

Are easy to operate

Question 2

(i), (iii), (ii), (iv), (v)

(iii), (ii), (i), (v), (iv)

(i), (v), (ii), (iv), (iii)

(v), (i), (iii), (iv), (ii)

Question 3

1 and 3

4 and 6

4 and 7

4, 7 and 8

Question 4

Chemically synthesised oligonucleotides that are complementary to the regions of DNA

Chemically synthesised oligonucleotides that are not complementary to the regions of DNA

Chemically synthesised, autonomously replicating circular DNA molecules

Specific sequences present on recombinant DNA

Question 5

Agrobacterium tumefaciens

Thermus aquaticus

Streptomyces albus

Escherichia coli

Question 6

Pfu polymerase isolated from Pyrococcus furiosus

Tli polymerase (vent polymerase) isolated from Thermococcus litoralis

Both (a) and (b)

None of these

Question 7

Transformation

Electrophoresis

Downstream processing

Upstream processing

Question 8

Bromophenol blue

Chilled ethanol

Ethidium bromide

Both A and C

Question 9

A - i, B - ii, C - iii

A - i, B - iii, C - ii

A - ii, B - iii, C - i

A - iii, B - i, C - ii

Question 10

of the inability to excise introns and destruction by bacterial restriction enzymes

of high pH present in bacterial cells

of inappropriate insertion of genes

both (a) and (b)

Question 11

A-(iv), B-(i), C-(iii), D-(ii)

A-(i), B-(iii), C-(ii), D-(iv)

A-(ii), B-(i), C-(iii), D-(iv)

A-(iv), B-(ii), C-(i), D-(iii)

Question 12

(i), (iii) and (vi)

(i), (iii) and (v)

(ii), (iii) and (v)

(i), (iv) and (v)

Question 13

(i) and (iii)

Only (iii)

(i) and (ii)

(i) and (ii) and (iv)

Question 14

(ii) and (iii)

(iii) and (iv)

(i) and (iii)

(i) and (ii)

Question 15

Produce restriction enzymes

Excise introns from the RNA transcript

Remove methyl groups

Reproduce more rapidly

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