Biotechnology & its Applications Test

Question 1
4 / -1

Which of the following statements regarding the structure of proinsulin and mature insulin are not correct?
(i) Proinsulin is made up of three polypeptide chains- A, B and C.
(ii) C - polypeptide chain with 33 amino adds is removed prior to insulin formation.
(iii) Mature insulin is made up of 51 amino acids arranged in two polypeptide chains- A and B.
(iv) Polypeptide chain A has 30 amino acids and polypeptide chain B has 21 amino acids.
(v) Polypeptide chains A and B are interconnected by only one S−S linkage.

A
(i) and (ii)

B
(iii) and (iv)

C
(iv) and (v)

D
(iii), (iv) and (v)

Question 2
4 / -1

Which of the following statements regarding gene therapy is/are correct?

A
It is an application of biotechnology, in which a defective gene is manipulated by introduction of a normal, healthy and functional gene

B
The genetic disorders that are being investigated for gene therapy, range from sickle-cell anaemia to severe combined immuno-deficiency (SCID)

C
The first clinical gene therapy was given in 1990 to a 4-year old girl with adenosine deaminase (ADA) deficiency

D
All of these

Question 3
4 / -1

Read the given statements and select the correct options
Statement 1: In recombinant DNA technology, human genes are often transferred into bacteria (prokaryotes) or yeast (eukaryote).
Statement 2: Both bacteria and yeast multiply very fast to form huge population which expresses the desired gene.

A
Both statements 1 and 2 are correct and statement 2 is the correct explanation of statement 1.

B
Both Both statements 1 and 2 are correct but statement 2 is not the correct explanation of statement 1.

C
Statement 1 is correct and statement 2 is incorrect.

D
Both statements 1 and 2 are incorrect.

Question 4
4 / -1

Read the given statements and select the correct option
Statement 1: PCR technique is helpful in detecting bacterial and viral diseases even when symptoms of the disease are not yet visible
Statement 2: Very low concentrations of bacteria or viruses in human body can be detected by amplification of their nucleic acids using the PCR technique

A
Both statements 1 and 2 are correct and statement 2 is the correct explanation of statement 1.

B
Both Both statements 1 and 2 are correct but statement 2 is not the correct explanation of statement 1.

C
Statement 1 is correct and statement 2 is incorrect.

D
Both statements 1 and 2 are incorrect.

Question 5
4 / -1

DNA fingerprinting refer to

A
Molecular analysis of profiles of DNA samples

B
Analysis of DNA samples using imprinting devices

C
Techniques used for molecular analysis of different specimens of DNA

D
Techniques used for identification of fingerprints of individuals

Question 6
4 / -1

Given figure represents the maturation of pro-insulin into insulin. Identify the product A.

A
Polypeptide chain A

B
Polypeptide chain B

C
Polypeptide chain C

D
None of these

Question 7
4 / -1

Early detection of a disease is possible by

A
PCR

B
Gene therapy

C
Recombinant DNA technology and ELISA

D
both (a) and (c)

Question 8
4 / -1

Technique used to detect the DNA in a clone is

A
polymerase chain reaction

B
gel electrophoresis

C
chromatography

D
autoradiography

Question 9
4 / -1

Which of the following statements is not correct?

A
Insulin used for diabetic patients was earlier extracted from pancreas of slaughtered cattle and pigs which was more efficient than that the genetically engineered insulin

B
PCR technique is applied to detect HIV in Suspected AIDS patients and to detect mutations in genes in suspected cancer patients

C
Bone marrow transplantation requires periodic infusion of genetically engineered lymphocytes

D
Bio-remediation is the one of the applications of biotechnology

Question 10
4 / -1

What might be an advantage of beginning gene therapy prior to birth?

A
This would give the body plenty of time to utilise the new genes

B
The body would not reject it as it has not yet recognised ‘self’

C
The cells being extremely young, are more receptive to gene therapy

D
Probably there is not any advantage

Question 11
4 / -1

Which of the following companies started selling humulin in the year 1983?

A
Eli Lilly

B
Genetech

C
GEAC

D
None of these

Question 12
4 / -1

Which of the following statements is incorrect about gene therapy in ADA deficiency?

A
Lymphocytes from patient's blood are taken out and cultured

B
A functional ADA−cDNA is introduced into these lymphocytes

C
Lymphocytes are then introduced in the body of patient

D
Patient does not require periodic infusion of genetically engineered lymphocytes

Question 13
4 / -1

The study of all the proteins coded by the genome is called as

A
Proteomics

B
Genomics

C
Gene library

D
Proteology

Question 14
4 / -1

Gene can therapy can be referred to as

A
pre - clinical testing for inherited diseases in newborns

B
treatment of diseases caused by genetic defect

C
genetic engineering using rDNA technology

D
cancer treatment using in vitro cultured stem cells

Question 15
4 / -1

For effective treatment of a disease

A
early diagnosis is required but understanding of its pathophysiology is not required.

B
early diagnosis is not required but understanding of its pathophysiology is required.

C
early diagnosis and understanding of its pathophysiology are required.

D
neither early diagnosis nor understanding of its pathophysiology is required.

Question 16
4 / -1

Some of the steps involved in the production of Humulin are given below. Choose the correct sequence.
(i) Synthesis of gene (DNA) for human insulin artificially.
(ii) Culturing recombinant E.coli in bioreactors.
(iii) Purification of humulin.
(iv) Insertion of human insulin gene into plasmid.
(v) Introduction of recombinant plasmid into E.coli.
(vi) Extraction of recombinant gene product from E.coli.

A
i, iii, v, vi, ii, iv

B
ii, i, iv, iii. v, vi

C
i, iv, v, ii, vi, iii

D
iii, v, ii, i, vi, iv

Question 17
4 / -1

During the processing of proinsulin into the mature insulin

A
C -peptide is added to proinsulin

B
C-peptide is removed from proinsulin

C
B-peptide is added to proinsulin

D
B-peptide is removed from proinsulin

Question 18
4 / -1

Study the following steps which are followed during the process of gene therapy while treating a patient of SCID
(i) Retrovirus infects lymphocytes extracted from bone marrow of the patient and cultured.
(ii) Engineered cells are injected into patient's bone marrow.
(iii) Normal allele is inserted into a retrovirus.
(iv) Retrovirus makes a DNA copy of its RNA. This DNA carrying the normal allele gets inserted into the chromosome of the host cell.
Arrange the above-given steps in correct sequence and select the correct answer.

A
(iii), (i), (ii), (iv)

B
(iii), (i), (iv), (ii)

C
(iv), (ii), (iii), (i)

D
(iv), (iii), (i), (ii)

Question 19
4 / -1

A doctor while operating on an HIV (+)ve patient accidentally cuts himself with a scalpel. Suspecting himself to have contracted the virus which test will he take to rule out/confirm his suspicion?

A
PCR

B
Routine urine examination

C
TLC

D
DLC

Question 20
4 / -1

Second generation vaccines are prepared by recombinant DNA technology. Which out of the following are the examples of such vaccines?

A
Hepatitis B virus vaccine

B
Herpes virus vaccine

C
Salk's polio vaccine

D
Both (a) and (b)

Submit Test

Biotechnology &...

Question 1

(i) and (ii)

(iii) and (iv)

(iv) and (v)

(iii), (iv) and (v)

Question 2

It is an application of biotechnology, in which a defective gene is manipulated by introduction of a normal, healthy and functional gene

The genetic disorders that are being investigated for gene therapy, range from sickle-cell anaemia to severe combined immuno-deficiency (SCID)

The first clinical gene therapy was given in 1990 to a 4-year old girl with adenosine deaminase (ADA) deficiency

All of these

Question 3

Both statements 1 and 2 are correct and statement 2 is the correct explanation of statement 1.

Both Both statements 1 and 2 are correct but statement 2 is not the correct explanation of statement 1.

Statement 1 is correct and statement 2 is incorrect.

Both statements 1 and 2 are incorrect.

Question 4

Both statements 1 and 2 are correct and statement 2 is the correct explanation of statement 1.

Both Both statements 1 and 2 are correct but statement 2 is not the correct explanation of statement 1.

Statement 1 is correct and statement 2 is incorrect.

Both statements 1 and 2 are incorrect.

Question 5

Molecular analysis of profiles of DNA samples

Analysis of DNA samples using imprinting devices

Techniques used for molecular analysis of different specimens of DNA

Techniques used for identification of fingerprints of individuals

Question 6

Polypeptide chain A

Polypeptide chain B

Polypeptide chain C

None of these

Question 7

PCR

Gene therapy

Recombinant DNA technology and ELISA

both (a) and (c)

Question 8

polymerase chain reaction

gel electrophoresis

chromatography

autoradiography

Question 9

Insulin used for diabetic patients was earlier extracted from pancreas of slaughtered cattle and pigs which was more efficient than that the genetically engineered insulin

PCR technique is applied to detect HIV in Suspected AIDS patients and to detect mutations in genes in suspected cancer patients

Bone marrow transplantation requires periodic infusion of genetically engineered lymphocytes

Bio-remediation is the one of the applications of biotechnology

Question 10

This would give the body plenty of time to utilise the new genes

The body would not reject it as it has not yet recognised ‘self’

The cells being extremely young, are more receptive to gene therapy

Probably there is not any advantage

Question 11

Eli Lilly

Genetech

GEAC

None of these

Question 12

Lymphocytes from patient's blood are taken out and cultured

A functional ADA−cDNA is introduced into these lymphocytes

Lymphocytes are then introduced in the body of patient

Patient does not require periodic infusion of genetically engineered lymphocytes

Question 13

Proteomics

Genomics

Gene library

Proteology

Question 14

pre - clinical testing for inherited diseases in newborns

treatment of diseases caused by genetic defect

genetic engineering using rDNA technology

cancer treatment using in vitro cultured stem cells

Question 15

early diagnosis is required but understanding of its pathophysiology is not required.

early diagnosis is not required but understanding of its pathophysiology is required.

early diagnosis and understanding of its pathophysiology are required.

neither early diagnosis nor understanding of its pathophysiology is required.

Question 16

i, iii, v, vi, ii, iv

ii, i, iv, iii. v, vi

i, iv, v, ii, vi, iii