Biotechnology: Principle & Processes Test 9

Result

Biotechnology: Principle & Processes Test 9

Score
-
out of -
Rank
-
out of -

TIME Taken - -

SHARING IS CARING

If our Website helped you a little, then kindly spread our voice using Social Networks. Spread our word to your readers, friends, teachers, students & all those close ones who deserve to know what you know now.

Weekly Quiz Competition

Question 1
1 / -0

A schematic representation of polymerase chain reaction (PCR) up to the extension stage is given below.
Q. Name the process ‘a’

A
A. Termination

B
B. Annealing

C
C. Denaturation

D
D. Extension

Solution

Denaturing is the first step of PCR which involves the breaking of phosphate bonds between the DNA base pairs at temperature 80°C.
Question 2
1 / -0

A schematic representation of polymerase chain reaction (PCR) up to the extension stage is given below.
Q. PCR technique is best for :

A
A. DNA synthesis

B
B. Protein amplification

C
C. DNA amplification

D
D. DNA ligation.

Solution

PCR techniques are helpful to detect very minute traces of virus or bacterial DNA and other multiple copies by DNA amplification.
Question 3
1 / -0

A schematic representation of polymerase chain reaction (PCR) up to the extension stage is given below.
Q. Identify ‘b’

A
A. Termination

B
B. Annealing primer

C
C. Denaturation

D
D. Extension

Solution

The primer binds on the DNA that initiates the polymerisation with the help of tag polymerase enzyme at 72°C temperature.
Question 4
1 / -0

A schematic representation of polymerase chain reaction (PCR) up to the extension stage is given below.
Q. Which among the following is not an application of PCR ?

A
A. ELISA

B
B. Diagnosis of pathogens

C
C. DNA fingerprinting

D
D. In palaeontology.

Solution

PCR techniques are used to identify traces of DNA but it can not diagnose pathogens or organisms.
Question 5
1 / -0

A schematic representation of polymerase chain reaction (PCR) up to the extension stage is given below.
Q. Which of the following has popularized the PCR (Polymerase Chain Reaction) ?

A
A. Easy availability of DNA template

B
B. Availability of synthetic primers

C
C. Availability of cheap deoxyribonucleotides

D
D. Availability of thermostable DNA polymerase.

Solution

Tag polymerase enzymes help to maintain the stability of DNA to be used repeatedly at high temperature in the PCR.
Question 6
1 / -0

Read the following text and answer the following question on the basis of the same:
The term biotechnology refers to the use of living organisms or their products to modify human health and their human environment. For example, ‘testtube’ programme, synthesis of a gene or correcting a defective gene are all part of biotechnology. The basis of modern biotechnology is genetic engineering and maintenance of sterile conditions. Genetic engineering is the technique that alter the chemistry of genetic material i.e. DNA and RNA, then this genetic material is introduced into host organisms, which alter the phenotype of the host organism.
Q. Discovery of _________ molecule made genetic engineering possible.

A
A. Restriction exonuclease

B
B. Restriction exonuclease

C
C. Ribozyme

D
D. DNA polymerase

Solution

Restriction endonuclease acts as a molecular sensor that gets the DNA from specific nucleotides and gives desired fragments of DNA.
Question 7
1 / -0

Read the following text and answer the following question on the basis of the same:
The term biotechnology refers to the use of living organisms or their products to modify human health and their human environment. For example, ‘testtube’ programme, synthesis of a gene or correcting a defective gene are all part of biotechnology. The basis of modern biotechnology is genetic engineering and maintenance of sterile conditions. Genetic engineering is the technique that alter the chemistry of genetic material i.e. DNA and RNA, then this genetic material is introduced into host organisms, which alter the phenotype of the host organism.
Q. The cutting of DNA at specific locations became possible with the discovery of :

A
A. Ligases

B
B. Restriction enzymes

C
C. Probes

D
D. Selectable markers.

Solution

Restriction enzymes are the molecular scissors that cut the DNA from a specific recognition site.
Question 8
1 / -0

Read the following text and answer the following question on the basis of the same:
The term biotechnology refers to the use of living organisms or their products to modify human health and their human environment. For example, ‘testtube’ programme, synthesis of a gene or correcting a defective gene are all part of biotechnology. The basis of modern biotechnology is genetic engineering and maintenance of sterile conditions. Genetic engineering is the technique that alter the chemistry of genetic material i.e. DNA and RNA, then this genetic material is introduced into host organisms, which alter the phenotype of the host organism.
Q. The recognition sequence of the first restriction enzyme isolated was ________ base pair long.

A
A. four

B
B. six

C
C. five

D
D. two

Solution

ECoRI is isolated as the first restriction of the GAATAC nucleotide base pair.
Question 9
1 / -0

Read the following text and answer the following question on the basis of the same:
The term biotechnology refers to the use of living organisms or their products to modify human health and their human environment. For example, ‘testtube’ programme, synthesis of a gene or correcting a defective gene are all part of biotechnology. The basis of modern biotechnology is genetic engineering and maintenance of sterile conditions. Genetic engineering is the technique that alter the chemistry of genetic material i.e. DNA and RNA, then this genetic material is introduced into host organisms, which alter the phenotype of the host organism.
Q. DNA fragments are :

A
Positively charged

B
Neutral

C
Negatively charged

D
Either positively or negatively charged depending on their size.

Solution

DNA has PO₄^–as a negative end So, DNA is negatively charged.
Question 10
1 / -0

Read the following text and answer the following question on the basis of the same:
The term biotechnology refers to the use of living organisms or their products to modify human health and their human environment. For example, ‘testtube’ programme, synthesis of a gene or correcting a defective gene are all part of biotechnology. The basis of modern biotechnology is genetic engineering and maintenance of sterile conditions. Genetic engineering is the technique that alter the chemistry of genetic material i.e. DNA and RNA, then this genetic material is introduced into host organisms, which alter the phenotype of the host organism.
Q. The specific DNA sequence where EcoRI cuts is :

A
A. GATTCG

B
B. GAATTC

C
C. GTTCAA

D
D. TTCCAA

Solution

ECoRI recognises GAATAC base pairs that cuts between G and A.
Question 11
1 / -0

Read the following and answer the question given below :
Restriction endonuclease was isolated for the first time by W. Aber in 1962 in bacteria. Restriction endonucleases cut the DNA duplex at specific points therefore they are also called molecular scissors or biological scissors. Three types of restriction endonucleases are Type 1. Type ll and Type III but only Type II restriction endonucleases are used in recombinant DNA technology. Restriction endonuclease EcoR I recognises the base sequence GAATTC In DNA duplex and cut strands between G and A.
Q. Only type II restriction enzymes are used in gene manipulation because:

A
A. ATP is not required for cleaving

B
B. it consists of three different subunits

C
C. it makes cleavage or cut in both the strands of DNA molecule

D
D. both (A) and (C)

Solution

Type II enzymes are simpler and don't require ATP as an energy source, unlike Type I and it makes cleavage or cut in both the strands of DNA molecule.
Question 12
1 / -0

Read the following and answer the question given below :
Restriction endonuclease was isolated for the first time by W. Aber in 1962 in bacteria. Restriction endonucleases cut the DNA duplex at specific points therefore they are also called molecular scissors or biological scissors. Three types of restriction endonucleases are Type 1. Type ll and Type III but only Type II restriction endonucleases are used in recombinant DNA technology. Restriction endonuclease EcoR I recognises the base sequence GAATTC In DNA duplex and cut strands between G and A.
Q. Restriction endonucleases are also called as molecular or biological scissors because:

A
A. they cleave base pairs of DNA only at their terminal ends

B
B. they cleave one or both the strands of DNA

C
C. they act only on single stranded DNA

D
D. none of these

Solution

Restriction endonucleases naturally target double stranded DNA and could cleave one or both strands of the same.
Question 13
1 / -0

Read the following and answer the question given below :
Restriction endonuclease was isolated for the first time by W. Aber in 1962 in bacteria. Restriction endonucleases cut the DNA duplex at specific points therefore they are also called molecular scissors or biological scissors. Three types of restriction endonucleases are Type 1. Type ll and Type III but only Type II restriction endonucleases are used in recombinant DNA technology. Restriction endonuclease EcoR I recognises the base sequence GAATTC In DNA duplex and cut strands between G and A.
Q. Which of the following ions are used by restriction endonucleases for restriction?

A
A. Mg²⁺ ions

B
B. Mn²⁺ ions

C
C. Na⁺ions

D
D. K⁺ions

Solution

The restriction endonuclease binds two magnesium ions. One of these ions binds to the phosphate group where the cleavage occurs and is required for catalysis.
Question 14
1 / -0

Read the following and answer the question given below :
Restriction endonuclease was isolated for the first time by W. Aber in 1962 in bacteria. Restriction endonucleases cut the DNA duplex at specific points therefore they are also called molecular scissors or biological scissors. Three types of restriction endonucleases are Type 1. Type ll and Type III but only Type II restriction endonucleases are used in recombinant DNA technology. Restriction endonuclease EcoR I recognises the base sequence GAATTC In DNA duplex and cut strands between G and A.
Q. Which type of restriction endonucleases is used most in genetic engineering?

A
A. Type I

B
B. Type II

C
C. Type III

D
D. Type IV

Solution

Type I and Type III are complex and have only a limited role in genetic engineering. Type II restriction endonucleases are used mostly as the cutting enzymes in gene cloning.
Question 15
1 / -0

Read the following and answer the question given below :
Restriction endonuclease was isolated for the first time by W. Aber in 1962 in bacteria. Restriction endonucleases cut the DNA duplex at specific points therefore they are also called molecular scissors or biological scissors. Three types of restriction endonucleases are Type 1. Type ll and Type III but only Type II restriction endonucleases are used in recombinant DNA technology. Restriction endonuclease EcoR I recognises the base sequence GAATTC In DNA duplex and cut strands between G and A.
Q. Restriction endonuclease was isolated for the first time from a:

A
A. plant cell

B
B. animal cell

C
C. prokaryotic cell

D
D. germinal cell

Solution

Restriction endonuclease was isolated for the first time from the bacterium Haemophilus influenzae (prokaryotic cell).

User

Question Analysis

Correct -
Wrong -
Skipped -

My Perfomance

Score
-
out of -
Rank
-
out of -

Re-Attempt Weekly Quiz Competition

Biotechnology: Principle & Processes Test 9

Result

Biotechnology: Principle & Processes Test 9

Score

-

Rank

-

SHARING IS CARING

Question 1

A. Termination

B. Annealing

C. Denaturation

D. Extension

Solution

Question 2

A. DNA synthesis

B. Protein amplification

C. DNA amplification

D. DNA ligation.

Solution

Question 3

A. Termination

B. Annealing primer

C. Denaturation

D. Extension

Solution

Question 4

A. ELISA

B. Diagnosis of pathogens

C. DNA fingerprinting

D. In palaeontology.

Solution

Question 5

A. Easy availability of DNA template

B. Availability of synthetic primers

C. Availability of cheap deoxyribonucleotides

D. Availability of thermostable DNA polymerase.

Solution

Question 6

A. Restriction exonuclease

B. Restriction exonuclease

C. Ribozyme

D. DNA polymerase

Solution

Question 7

A. Ligases

B. Restriction enzymes

C. Probes

D. Selectable markers.

Solution

Question 8

A. four

B. six

C. five

D. two

Solution

Question 9

Positively charged

Neutral

Negatively charged

Either positively or negatively charged depending on their size.

Solution

Question 10

A. GATTCG

B. GAATTC

C. GTTCAA

D. TTCCAA

Solution

Question 11

A. ATP is not required for cleaving

B. it consists of three different subunits

C. it makes cleavage or cut in both the strands of DNA molecule

D. both (A) and (C)

Solution

Question 12

A. they cleave base pairs of DNA only at their terminal ends

B. they cleave one or both the strands of DNA

C. they act only on single stranded DNA

D. none of these

Solution

A. Mg²⁺ ions

B. Mn²⁺ ions

C. Na⁺ions

D. K⁺ions