Self Studies

Biotechnology: ...

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  • Question 1
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    Directions : In the following questions a statement of assertion (A) is followed by a statement of reason (R). Mark the correct choice as :

    Assertion (A) : β-galactosidase coding sequence acts as a selectable marker.

    Reason (R) : Enzyme galactosidase converts the galactose into lactose.

  • Question 2
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    Directions : In the following questions a statement of assertion (A) is followed by a statement of reason (R). Mark the correct choice as :

    Assertion (A) : DNA is a positively charged molecule.

    Reason (R) : DNA moves towards the positive electrode (anode).

  • Question 3
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    Directions : In the following questions a statement of assertion (A) is followed by a statement of reason (R). Mark the correct choice as :

    Assertion (A) : Thermus aquaticus, is used in PCR technique.

    Reason (R) : It is a heat-stable DNA polymerase.

  • Question 4
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    Directions : In the following questions a statement of assertion (A) is followed by a statement of reason (R). Mark the correct choice as :

    Assertion (A) : E. coli having pBR322 with DNA insert at BamHI site cannot grow in medium containing tetracycline.

    Reason(R) : Recognition site for BamH I is present in TetR region of pBR322.

  • Question 5
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    Directions : In the following questions a statement of assertion (A) is followed by a statement of reason (R). Mark the correct choice as :

    Assertion (A) : Any fragment of DNA, when linked to the ori region, can be initiated to replicate.

    Reason (R) : Ori is a genetic sequence that acts as the initiation site for replication of DNA.

  • Question 6
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    Directions : In the following questions a statement of assertion (A) is followed by a statement of reason (R). Mark the correct choice as :

    Assertion (A) : EcoRI is a restriction endonuclease enzyme.

    Reason (R) : Exonuclease removes nucleotides from the ends of DNA.

  • Question 7
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    Directions : In the following questions a statement of assertion (A) is followed by a statement of reason (R). Mark the correct choice as :

    Assertion (A) : Restriction enzymes belong to class nucleases.

    Reason (R) : Nucleases are of two kinds : exo and endonucleases. Exonucleases remove nucleotides within the DNA.

  • Question 8
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    Directions : In the following questions a statement of assertion (A) is followed by a statement of reason (R). Mark the correct choice as :

    Assertion (A) : It is essential to have few cloning sites in the cloning vector.

    Reason(R) : It helps in identifying and eliminating non-transformants and selectively permitting the growth of the transformants.

  • Question 9
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    Directions : In the following questions a statement of assertion (A) is followed by a statement of reason (R). Mark the correct choice as :

    Assertion (A) : Agarose gel electrophoresis is used to check the progression of a restriction enzyme digestion.

    Reason (R) : Restriction enzyme digestions are performed by incubating purified DNA molecules with restriction enzymes.

  • Question 10
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    Directions : In the following questions a statement of assertion (A) is followed by a statement of reason (R). Mark the correct choice as :

    Assertion (A) : A primer is a small segment of DNA that binds to a complementary strand of DNA.

    Reason(R) : Primers are necessary to stop the functioning of DNA polymerase enzymes and, therefore, are necessary in polymerase chain reaction.

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